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Shodex IEC DEAE-825, Operation Manual, Page: 5 / 6

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Shodex IEC DEAE-825 Operation Manual Download Page 5

ver.211129E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

5 

9. Column Inspection

 

Inspection method is described in the Certificate of Analysis (CoA).   
Theoretical Plate Number (N) and Asymmetry Factor (Fas) were calculated using the below equations.   

 

(1) Theoretical Plate Number (N) 

 

 

(2) Asymmetry Factor (Fas) 

 

 

 

10. Additional Warnings 

(1) Do not remove end fittings. 

 

(2) Do not make a strong impact on the column. Do not drop or hit the column on a hard surface. 

 

Refer to the Shodex™ website (https://www.shodex.com/) for product details and their applications.   
For additional assistance, contact the distributor from whom you purchased the column or contact your regional 
Shodex

 support office (https://www.shodex.com/en/support_office/list/). 

 
 

 

Summary of Contents for IEC DEAE-825

Page 1: ...umber written on the column package column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex IEC DEAE 825 is a weak anion exchange column filled with hydrophilic polymer based gel modified with diethylaminoethyl group It is an ideal column for analyzing various proteins peptides and components of nucleic aci...

Page 2: ...lt When using highly corrosive salts such as sodium chloride wash out the salts at the end of analysis The metal parts of the devices and or the columns may rust Column pressure is influenced by the eluent composition flow rate and column temperature When changing the eluent compositions adjust the flow rate and column temperature so that the column pressure remains below the usable maximum pressu...

Page 3: ...orate the column 7 2 Column Installation 1 Connect the column to LC system by following the flow direction arrow indicated on the column name tag 2 Make sure to insert the tubing all the way to the end fitting and secure it with the male nut It is important that there is no extra space between the tubing and the column side of the end fitting Presence of an extra space will let the sample to sprea...

Page 4: ...are often caused by insoluble or adsorbing components present in the eluent and reagents being deposited inside the column These problems may be resolved by cleaning the column When washing the column let the washing solution flow from the column outlet go directly into the waste container and not let the solution go through the detector Cleaning Method 1 Insoluble components that block the column...

Page 5: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Page 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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