Shodex C18U 2B Operation Manual Download Page 1

ver.210601E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 C18U 2B, 2D 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 

 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product.   

Shodex™ C18U series is reverse phase chromatography column in which octadecyl group is bonded to organic 
and inorganic hybrid gel. Compared to conventional silica-based reversed phase columns, it has excellent 
separation performance and high durability, so it can be used under a variety of analytical conditions and is 
suitable for the separation of a wide variety of compounds. 
 

2. Column Components   

                 

 

 
 
 
 
 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle Size (

μ

m) *Carbon 

(%) 

I.D. Length 

F6654011 

C18U 2B 

2.0 

 

50 1.9 

20 

F6654012 

C18U 2D 

2.0 150 

1.9 

20 

 

  *Includes 8% carbon content of organic and silica hybrid gel 

 

Base Material 

: Spherical organic and silica hybrid particles modified with octadecyl group

 

Column Housing  : SUS-316 
Screw Type 

: Internally-threaded type No.10-32 UNF (Parker Type) 

Shipping Solvent : Acetonitrile 

 
 
 
 
 

Summary of Contents for C18U 2B

Page 1: ...tten on the column package column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex C18U series is reverse phase chromatography column in which octadecyl group is bonded to organic and inorganic hybrid gel Compared to conventional silica based reversed phase columns it has excellent separation performance an...

Page 2: ...n 1 Degas the eluent fully to prevent the formation of air bubbles 2 Presence of small debris or insoluble substances may result in deterioration of the column and or they appear as noise on the chromatograms Filter the eluent with a 0 2 μm disposable filter to prevent the problems Attention Whenever water is required use ultra pure water freshly generated by a water purification system or water f...

Page 3: ...is miscible soluble to both eluents and then replace it with the desired eluent e g When replacing highly concentrated buffer solution or salt solution to water acetonitrile first run water and then replace it with the eluent Attention If the eluent left in the system is not compatible with the column to be used it may damage the column A drastic change in the eluent compositions may remove substa...

Page 4: ...de the column These problems may be resolved by cleaning the column Make sure to clean the guard and the analytical columns separately When washing the column let the washing solution flow from the column outlet go directly into the waste container and not let the solution go through the detector Cleaning Method 1 Insoluble components that block the column inlet may be removed by reversing the flo...

Page 5: ...t on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support office https www shodex com en support_office list ...

Page 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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