Shodex Asahipak NH2P-40 2E Operation Manual Download Page 1

ver.211215E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 Asahipak

 NH2P-40 2E 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 

 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 Asahipak

 NH2P series is polymer-based 

chromatography columns modified with amino group. The column is suitable for the analyses that require HILIC 
or ion exchange mode separations. 

 

 

2. Column Components   
 
 
 
 

 

               

 

 

 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle 

Size (

μ

m) 

Theoretical Plate Number 

(Per Column) 

I.D. Length 

F7630010 

Asahipak NH2P-40 2E 

2.0 250 

 7,000   

 

Base Material 

: Spherical porous particles of polyvinyl alcohol modified with amino group

 

Column Housing  : SUS-316   
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : Acetonitrile 
 
 
 
 
 
 
 
 

Summary of Contents for Asahipak NH2P-40 2E

Page 1: ... Please visually inspect the package and outside of the column for any damage 2 Please check if product name and serial number written on the column package column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex Asahipak NH2P series is polymer based chromatography columns modified with amino group The colu...

Page 2: ...as noise on the chromatograms Filter the eluent with a 0 45 μm disposable filter to prevent the problems Attention Whenever water is required use ultra pure water freshly generated by a water purification system or water from a newly opened HPLC grade distilled water bottle Use HPLC grade organic solvent whenever possible Solvents left in an opened bottle for a long time should not be used The con...

Page 3: ...t the tubing all the way to the end fitting and secure it with the male nut It is important that there is no extra space between the tubing and the column side of the end fitting Presence of an extra space will let the sample to spread out and may result in wide peaks 3 Set the initial flow rate at 0 1 mL min and start the system If using the column at an elevated temperature keep a low flow rate ...

Page 4: ...Cleaning Method 1 7 4 Column Cleaning Problems in peak shapes and elution time changes or elevated column pressure etc are often caused by insoluble or adsorbing components present in the eluent and reagents being deposited inside the column These problems may be resolved by cleaning the column When washing the column let the washing solution flow from the column outlet go directly into the waste ...

Page 5: ...olumn Inspection Inspection method is described in the Certificate of Analysis CoA Theoretical Plate Number N and Asymmetry Factor Fas were calculated using the below equations 1 Theoretical Plate Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website...

Page 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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