Sequoia LISST-200X User Manual Download Page 1

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LISST-200X

Particle Size Analyzer

Including

LISST-HAB & LISST-Black

User’s Manual

Version 2.2

December, 2021

2700 Richards Road, Suite 107

Bellevue, WA 98005

USA

Telephone:

(+1) 855-753-3313

Store Software

USB Card Here

Summary of Contents for LISST-200X

Page 1: ...200X Particle Size Analyzer Including LISST HAB LISST Black User s Manual Version 2 2 December 2021 2700 Richards Road Suite 107 Bellevue WA 98005 USA Telephone 1 855 753 3313 Store Software USB Card...

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Page 3: ...LISST 200X User s Manual Page iii This document is copyrighted by Sequoia Scientific Inc...

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Page 5: ...step instructions for using and caring for the instrument The Appendices contain specialized details specifications and information about options Warranty See the product warranty at http sequoiasci c...

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Page 7: ...Data Quality Control 48 12 Real Time Data Processing with LISST 200X Software 50 13 Autonomous Real time Data Processing 52 14 Collecting Data from an External Analog Sensor 55 15 Configuring the LIS...

Page 8: ...Page viii LISST 200X User s Manual Intentionally blank...

Page 9: ...aylight rejection filter at the focal plane of a receive lens at the same angle from lens axis see figure Distance from lens axis in the lens focal plane corresponds to scattering angle The photodetec...

Page 10: ...For details on the background measurement procedure see Saving and Evaluating Clean Water Backgrounds on page 29 Ambient Light Rejection The laser diffraction method requires that the light arriving o...

Page 11: ...Newer LISST 200X s starting with serial number 2131 and older units with upgraded circuitry contain more powerful processors that can do full PSD processing in real time The processed data can be stor...

Page 12: ...equoiaSci com The LISST 200X instrument is a laser diffraction device It consists of optics for producing a collimated laser beam a specially constructed detector array electronics for signal pre ampl...

Page 13: ...t can be configured to produce analog outputs or to receive analog inputs The Analog Output Configuration allows the LISST 200X to receive power from a CTD and to send two analog voltages indicating t...

Page 14: ...real time observation of the size distributions Included Accessories The instrument is shipped pre aligned and tested A small chamber is provided for obtaining measurements of background scattered lig...

Page 15: ...e for the first time Inside you will find the following User s Manual USB memory card credit card size with the software LISST 200X instrument Plastic Instrument stands Small Volume Test Chamber USB C...

Page 16: ...connector Used for BioBlock an anti biofouling accessory 5 pin connector Used for serial communication and external power 6 pin connector Used for analog in out and digital in out signals See Appendi...

Page 17: ...a mild soap solution e g mild hand soap liquid dish soap and then rinsing off all soap residue with clean particle free water such as deionized water distilled water or bottled drinking water The win...

Page 18: ...l surfaces Pressurized filtered water can also contain dissolved gas Often the best source of clean water is bottled drinking water Step 5 Install LISST 200X Software At this point the instrument is r...

Page 19: ...7 Establish Communication with the LISST 200X The LISST 200X does not have an internal battery Power is supplied to the instrument via a battery housing or USB cable Please note that the LISST 200X US...

Page 20: ...hat allows the user to communicate directly with the instrument using a set of commands To open the Terminal window click on the connected instrument message in the lower left then click on the wrench...

Page 21: ...background file will be automatically acquired from the instrument and displayed as a red line on the screen The Current Background is the last background that was collected and saved on the instrumen...

Page 22: ...omputer The LISST 200X handles the background files differently than the LISST 100X The background file is stored on the instrument and is saved as part of every data file that is recorded When proces...

Page 23: ...mpling Fixed Sample Rate or Burst You can also select the samples per average and sample rates on this screen Burst and Fixed Rate Modes The Burst and Fixed Sample Rate modes are used to save data to...

Page 24: ...tch External Digital Input and Time Delay Select the mode by clicking on the button next to its label Select the correct parameters as required For this example let s select the External Mechanical Sw...

Page 25: ...t which LISST 200X accessories are connected to the instrument The accessories must be selected here for them to function properly If there are no accessories select None Step 15 Upload Configuration...

Page 26: ...he instrument is now configured for deployment however it is not yet running You MUST explicitly start the samping program you just configured If you do not do that the LISST will never start sampling...

Page 27: ...ond If settings are set for a 30 second average every 15 minutes then the LED will illuminate for 30 seconds every 15 minutes Step 17 Collecting Data For our example we can move the white lever on the...

Page 28: ...elect a range of files Choose a location to offload the files to on the right side The files will be saved with names in the following format Ldddhhmm RBN where ddd is the day of the year hh is the ho...

Page 29: ...RTX Raw Data ASCII CSV Processed Data ASCII BGT Background File ASCII Every LISST 200X data file contains all the necessary information to process the file Therefore the software will automatically de...

Page 30: ...automatically You can also open any LISST 200X CSV file by selecting the View Process File button from the home page A display similar to the one below will open A list of available parameters is disp...

Page 31: ...f seconds between sample in the sample interval text box Even though data is displayed to the screen it is not saved until you select the Start Logging Button The Spherical Irregular Shape radio butto...

Page 32: ...ions on shipping NiMH batteries However the batteries will have a small drain when installed in the Small Battery housing Therefore it is recommended that they be removed until the battery is ready to...

Page 33: ...ge 52 In short the Auxiliary connector can be configured in one of four ways 1 Analog and Digital inputs 2 Dual Analog Inputs 3 Dual Analog Outputs or 4 Triple analog input When using the Analog or Di...

Page 34: ...bed if the instrument is subjected to shock or rough handling Evidence of shock rough handling will void the warranty Whenever in transit store the instrument in the provided padded shipping case If p...

Page 35: ...ISST 200X_Installer exe executable on the memory card Note that this software it is not compatible with Mac or Linux operating systems Your operating system must be Window XP or newer to run the softw...

Page 36: ...vers are being installed If you are unsure if the drivers installed you can install the drivers manually by running CDM USB Drivers exe located on the memory card that came with your instrument Comput...

Page 37: ...ld be at the same temperature Using water a significantly different temperature from the water can impact the quality of the background Stir the water well before obtaining a background to make sure t...

Page 38: ...suggested actions will be displayed Dirty water or windows will generally cause higher values across the middle rings Large bubbles or particles in the water can cause higher values on the inner rings...

Page 39: ...ved select the Save as BGT File button The LISST 200X handles the background files differently than the LISST 100X The background file is stored on the instrument and is saved as part of every data fi...

Page 40: ...mpute the PSD onboard in real time If your instrument has this feature an additional configuration Data Output will appear on this page See Autonomous Real time Data Processing on page 52 for more inf...

Page 41: ...his value is less than the minimum permitted the value will be changed to the minimum and the text will turn red A similar test is done on the Burst Interval setting 4 Select the Start Conditions tab...

Page 42: ...External Digital Input and the Start Condition is also one of these three options the Base Program on the instrument will return to waiting for the Start condition For example if the start and stop c...

Page 43: ...running You MUST run the sampling program in order to collect data But even when running the sampling program will wait for the programmed start condition before collecting data See Start and Stop Con...

Page 44: ...re not installed when the instrument a new instrument is shipped It is recommend that the batteries be removed from the housing when not in use Leaving the batteries in the housing will cause the batt...

Page 45: ...d is pushing against the batteries as shown in the picture above Batteries installed 4 Check that the o ring in the endcap and the mating surface on the main housing are clean Lightly grease with sili...

Page 46: ...end of the cable to the 5 pin Male bulkhead on the LISST 200X Power is now going to the LISST 200X If AutoStart is enabled the instrument may be active and waiting for the start condition Instrument...

Page 47: ...e new alkaline batteries can be left in the battery housing for storage However it is not recommended that fully discharged batteries be left in the battery housing during long term storage Battery re...

Page 48: ...e the surfaces as needed Align the tab in the center of the endcap to the slot in the post in the center of the pressure case Endcap re installed 5 Screw the connector endcap onto the pressure case wi...

Page 49: ...o the LISST 200X If AutoStart is enabled the instrument may be active and waiting for the start condition 7 If desired connect the Communications cable to the male 5 pin bulkhead connector on the Larg...

Page 50: ...ication in the LISST 200X program page 28 Software open communicating with LISST 200X 2 Select Offload Or Erase Files from the home page Shows a list of files currently stored onboard the instrument 3...

Page 51: ...parameter Select file types to be created 3 You must also select the inversion model to be used when processing the data Spherical randomly shaped or both For a description of the particle models see...

Page 52: ...ng into spreadsheets such as Excel etc The RTX file is a space delimited ASCII file containing the raw data from the LISST 200X data logger This is useful for looking at the raw data in a spreadsheet...

Page 53: ...from the home page Select multiple files to process by holding down either shift or ctrl Select Files 5 By default batch processing will use the background stored in the raw data files to process the...

Page 54: ...t extensions Several files will be created The CSV file is a space delimited ASCII file containing all the processed data The RTX file is a space delimited ASCII file containing the raw data from the...

Page 55: ...d file a window similar to the one shown below will appear Particle size distribution displayed on screen A list of available parameters is displayed on the left Clicking one of the parameters will op...

Page 56: ...e then your measurement is most likely taken in very clear water and or you have a bad background measurement obtained with dirty water and or dirty windows If your background measurement was obtained...

Page 57: ...the water is too clear Disregard these data Data with transmission values 99 5 should be disregarded 8 Summarizing Disregard data if transmission is 0 995 99 5 Disregard data if transmission is 0 10...

Page 58: ...y stored onboard the instrument will be used to process the data as it is collected in real time Be sure to collect a new background and store it onboard the instrument before beginning a real time se...

Page 59: ...s only effect the display and have no effect on the data that is saved in the CSV file Data from both particle models is always saved 5 Select an output directory and a base file name Several files wi...

Page 60: ...RESULT 1 Connect the instrument to the computer and establish communication in the LISST 200X program page 28 Communication established 2 Open the information panel at the bottom of the window and ch...

Page 61: ...ument in CSV format see Appendix C Data File Formats on page 79 o Transmit PSD In Real Time if checked PSD is computed onboard in real time A PSD data record is transmitted over the serial port immedi...

Page 62: ...LISST 200X Operation Details 54 LISST 200X User s Manual A message box will confirm the upload has been completed Configuration complete...

Page 63: ...the USB powered data cable If so you can augment the USB cable s power by connecting the large battery housing STEP ACTION RESULT 1 Determine current configuration of Auxiliary connector in LISST 200...

Page 64: ...ration of Auxiliary connector in LISST 200X software open the instrument status bar Look for the Endcap Configuration If it is not in the Dual Analog Output configuration it must be changed contact Se...

Page 65: ...atmospheric pressure temperature and even the orientation of the LISST 200X can affect the offset of the pressure sensor Therefore it is best to zero the sensor see page 32 immediately before starting...

Page 66: ...d stop thresholds there could be false starts and stops due to wave motion This could be prevented by setting the start depth deeper than the stop depth Time Stop After each sample or burst if the giv...

Page 67: ...ing data files the LISST 200X software uses YMODEM protocol at 115K baud The transfer rate can be changed in the settings of the LISST 200X software A slower speed may be required when downloading dat...

Page 68: ...stop condition Data PSDMODEL x Set model used for PSD processing if active SAVEPSD x Set whether PSD data will be saved in on board memory SI x Set sample interval SP x STOPCOND x Set stop condition S...

Page 69: ...Condition Data 0 Measurements Per Average 10 Sample Interval 1 seconds Burst Mode 0 Samples Per Burst 5 Burst Interval 25 minutes Transmit Raw Data 0 Lifetime Sample Count 529554 Lifetime Laser On Ti...

Page 70: ...1 21 06 43 Current Date Time 2019 01 09 22 12 50 Current Day of the Year 009 Supply voltage 14 74 V Operating mode Fixed rate mode AutoStart OFF Delay Start with 0 minute delay Stop on full memory or...

Page 71: ...and list Syntax HELP or Description Displays the list of command to the screen Example L200x HE LISST 200X Commands list of commands SW Display current Mechanical Switch position Syntax SW Description...

Page 72: ...ds between two consecutive bursts each burst composed of a number of samples per burst specified by the SB command Example L200x BI 900 New Seconds between Bursts 900 MA Set measurements per average S...

Page 73: ...t 3 meters If Stop Condition 2 Time Date Stop L200x PD 2016 12 31 23 59 59 Time stop at 2016 12 31 23 59 59 If Stop Condition 5 Fixed Number of Samples L200x PD 100 Stop after 100 samples PSDMODEL Set...

Page 74: ...value follows command prompts will be displayed for the value Example L200x SB 10 New Samples per Burst 10 SC Set Clock with time and date Syntax SC yyyy mm dd hh mm ss Where yyyy year mm month dd day...

Page 75: ...proceeds with sampling Example L200x SENSORWARM 10 Power output when used will turn on with 10 second warmup Power output during logging is on SI Set Sample Interval Syntax SI x where x is the number...

Page 76: ...s the start condition data to be used when the GO command is issued The start condition data is used with the Start Condition setting as follows If the Start Condition is Depth Start option 1 the inpu...

Page 77: ...e file from memory Example L200x DL L3231513 RBN Delete the file L3231513 RBN Y for Yes or N for No N Y Deleting L3231513 RBN Deleted Cautions WARNING Make sure the file being deleted has already been...

Page 78: ...ters repeat 20 times then messages about the quality of the data Notes The ZS command does not store the sample to a datafile ZZ Go into deep sleep mode minimum power consumption Syntax ZZ Description...

Page 79: ...ld always be collected before an experiment Techniques for measuring background Cleanliness It is essential to use clean bubble free water for the background measurement The water can be fresh or salt...

Page 80: ...f process is repeated 3 times per second Because of the time required for the laser and electronics to change between the on and off states rapidly changing ambient light may not be completely rejecte...

Page 81: ...ll cause the inner ring values to be much higher than the factory values and cause the transmitted laser power to decrease It must be noted that low laser power or high inner rings may not always indi...

Page 82: ...This anode must be exposed to the water for it to be effective When mounting the instrument be sure to electrically isolate the instrument from all other metal Any contact with other metal can greatl...

Page 83: ...ned shipping case Calibrations and Adjustments The LISST 200X should not require any adjustment or calibrations The pressure sensors can be re calibrated by Sequoia if desired The performance of the i...

Page 84: ...d 3V o Voltage tolerance without damage 12 to 12V Analog outputs availability depends on configuration o Output range 0 1 to 3 0 V o Resolution 1 mV o Output impedance 1 kOhm o Uncertainty 1 5 of read...

Page 85: ...LISST 200X User s Manual 77 Appendix A Technical Specifications...

Page 86: ...Shaped inversions Size bin Lower Upper Median 1 1 00 1 48 1 21 2 1 48 1 74 1 60 3 1 74 2 05 1 89 4 2 05 2 42 2 23 5 2 42 2 86 2 63 6 2 86 3 38 3 11 7 3 38 3 98 3 67 8 3 98 4 70 4 33 9 4 70 5 55 5 11...

Page 87: ...Processed Data ASCII Comma delimited file containing size distributions and meta data Details on page 80 For information about generating these files in the LISST 200X see Autonomous Real time Data P...

Page 88: ...46 Hour 47 Minute 48 Second 49 External analog input 2 V fluorometer 2 in LISST HAB LISST Black 50 Mean Diameter m calculated from fully processed size distribution 51 Total Volume Concentration PPM c...

Page 89: ...in LISST HAB LISST Black 40 Laser Reference counts 41 Depth 0 01 m count 10 m 42 Temperature 1 m C count 5 C 43 Year 44 Month 45 Day of month 46 Hour 47 Minute 48 Second 49 External Analog input 2 10...

Page 90: ...apply the necessary corrections and return the corrected data in a structure The syntax is as follows Datafile is the path to a binary RBN file downloaded from a LISST 200X Optionally you may specify...

Page 91: ...nd function inverts the corrected scattering to a particle size distribution The syntax is as follows The first argument is the corrected scattering cscat from the structure returned from getscat_L200...

Page 92: ...bConn cables with SubConn bulkhead connectors Sequoia s warranty does not cover any problems arising from mismatched connectors To Identify the connector type note 1 SubConn bulkhead connectors have b...

Page 93: ...in connector The photograph shows the placement of each connector The following text describes the detailed wiring for each connector Note that in new instruments starting in September 2019 these are...

Page 94: ...SubConn MCIL5F Connector Pin Use 1 Power Serial Ground 2 External Power In 9 to 24 V 3 Power Serial Ground 4 Serial Out to DB 9 Pin 2 5 Serial In to DB 9 Pin 3 BioBlock Connector 3 pin connector see...

Page 95: ...3 Digital In 2 4 12V out 5 Analog In 0 to 5V 6 Ground Analog Input Configuration Connector Pin Use 1 Ground 2 Analog In 3 0 to 5V 3 Ground 4 12V out 5 Analog In 1 0 to 5V 6 Analog In 2 0 to 5V Analog...

Page 96: ...ing Cable Part Number SubConn MCIL5M To LISST Connector 5 pin Female Bulkhead Connector Pin Use 1 Ground 2 Battery Power Out 9V nom 6 15V 3 Ground 4 Serial Out to DB 9 Pin 2 5 Serial In to DB 9 Pin 3...

Page 97: ...than assuming spheres The exact details of how this scattering model was established are described in a paper by Agrawal et al Light scattering by random shaped particles and consequences on measurin...

Page 98: ...format details see Appendix C Data File Formats The outputs are not calibrated to an absolute standard Firmware Configuration To check that the fluorometers will be powered whenever the LISST collect...

Page 99: ...inst the endcap and aligns to the flat surface Tighten the Clamp Screw 2 Position the Mid Support on the LISST 200X so the back edge is positioned on the pressure case chamfer as shown at right and th...

Page 100: ...lt and not recommended 7 Attach the LISST HAB Black cable to the sensors a The protrusion in the mold needs to be on the top side b Rotate the sensors to match the cable sockets and insert into the co...

Page 101: ...rement During the brief period less than 2 seconds while it is opening or closing the BioBlock roughly doubles the power consumption of the LISST 200X This is compatible with the large battery housing...

Page 102: ...lose whenever the sampling program pauses for at least 50 seconds NOTE your sampling program must either use burst mode or a long sample interval to schedule periodic gaps of at least 50 seconds when...

Page 103: ...sher and screw When tightening the screw hold the motor arm stationary to prevent forced rotation of the motor Slide the motor so its endcap seam is aligned with the front face of the large bracket Al...

Page 104: ...ceable effect on size distribution data inventing fine particles Transmissions as low as 10 may yield usable data but would require special care in processing and evaluating data If transmission is to...

Page 105: ...the PRM 1 Locate all the components of the LISST 200X Path Reduction Module as shown below The parts include quartz cylinder with retaining ring PRM Plate two white PRM Plugs and two 4 40 x 7 8 socke...

Page 106: ...29 The results should be close to the factory values However they may not receive a PASS result because of the instrument being in air WARNING A background performed in air is not acceptable for parti...

Page 107: ...the reduced path 1 Open the LISST 200X software and connect your instrument Press the Configure Instrument button to see this screen 2 Select the Pathlength Reduction Module option Note that the PRM c...

Page 108: ...und 3 Collect the background using the standard procedure as described on page 13 The instrument should be configured for use with the PRM prior to collecting a background If the instrument has been c...

Page 109: ...mage the optical surfaces It is also highly recommended to change the instrument configuration on the LISST 200X to reflect that the PRM is no longer installed 5 Maintaining and Storing the PRM 1 Remo...

Page 110: ...ed against the small window Remove the o ring to inspect it Replace it if it is brittle cracked or sticky 3 Install the black plastic retaining ring onto the quartz cylinder There should be about a 3m...

Page 111: ...a Scientific Q I think that I have a problem with my data and or my data processing and would like you to have a look at them can you do that What data do I need to send to you A We ll be happy to loo...

Page 112: ...reenshots for new LISST 200X software Version 1 5A Corrections in Appendix H Version 1 5 Add LISST HAB and LISST Black Version 1 4 Show change from Impulse to SubConn connectors Version 1 3B Note that...

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