Saxon 311008, Instruction Manual

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saxon Researcher Compact Biological Microscope 40x-1600x Instruction Manual
Page 5
Operating the microscope
Getting started
1. Switch on the power and the light(s) on your microscope should be illuminated. If your
microscope has a light intensity adjustment dial, ensure it is turned up to the brightest.
2. Place and align the specimen/object to the centre of the working stage and where possible,
hold it down with the stage clips.
Focusing
1. Rotate the nosepiece and place the objective lens with the lowest power (4x) in the optical
path. Make sure you hear a click that confirms it is in place.
2. If needed, loosen the locking screw located in between the stage and the arm to regulate
the range of movement of the stage.
3. Ensure the iris diaphragm is opened fully.
4. Push the spring loaded specimen holder to insert a specimen slide.
5. Adjust the slide with X and Y stage adjustment until the specimen is directly over the
condenser lens.
6. Looking through the eyepiece(s), adjust the interpupillary and turn the coarse focuser knob
to focus on the specimen until it comes into focus.
7. Re-adjust the focus with the fine focus knob until the image appears sharp and defined.
Changing magnification
Slowly turn the nosepiece clockwise or anticlockwise to the desired magnification until you hear a
click confirming the selected magnification is secured.
1. Ensure your specimen is sitting directly under the objective lens.
2. Rotate the coarse focuser knob, and then adjust the fine focus knob until the image comes
to focus.
Using 1000X magnification with immersion oil
To obtain maximum resolution with 100X objective lens, applying immersion oil between the slide
and the front lens is necessary.
1. Place one small drop of immersion oil on top of the cover slip, directly over where light
comes up from the condenser.
2. Slowly rotate the 100X objective lens into place. You should see the oil filling the space
between the lens and the slide. If bubbles appear, they can be removed by slightly rotating
the revolving nosepiece back and forth.
3. Adjust the focus with the fine focus knob only.
4. Always wipe and clean all parts that have come into contact with the immersion oil after use.
This is because oil could dry up on the lens surface and damage the microscope. Always
wipe with lens paper or a soft cotton cloth with the appropriate cleaning solution.
Note: The immersion oil should only be used for the 100X objective lens. Do not use the
immersion oil for any other objective lens as this may cause spherical and chromatic aberrations.