Roth 4849.1 Instructions For Use Manual Download Page 13

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Carl Roth GmbH+Co. KG

Schoemperlenstraße 3-5
76185 Karlsruhe
Postfach 100121
76231 Karlsruhe

Telefon: (+49)721/5606-0

Telefax: (+49)721/5606-149
E-Mail:

[email protected]

Internet:

www.carlroth.com

gh 05/2020

Complete electrophoresis system
ROTIPHORESE

PROfessional runVIEW

4849.1

Electrophoresis Station ROTIPHORESE

PROfessional runVIEW Base

Suitable for: Unit PROfessional III

4850.1

Electrophoresis Station ROTIPHORESE

PROfessional runVIEW Base MINI 1,

Suitable for: Unit PROfessional I

9958.1

Electrophoresis Station ROTIPHORESE

PROfessional runVIEW Base MINI 2,

Suitable for: Unit PROfessional II

9961.1

Summary of Contents for 4849.1

Page 1: ...zontal electrophoresis units from ROTH are designed for long term laboratory use and to obtain reproducible results Please spend a few moments reading the instruction manual thoroughly These units comply with the statutory CE safety rules 73 23 EEC Low voltage directive IEC 1010 1 1990 plus amendment 1 1992 EN 61010 1 1993 BS EN 61010 1 1993 Please verify that you received the unit completely and ...

Page 2: ...ruction manual The lid is suitable to electrophoresis unit ROTIPHORESE PROfessional III Art No 4850 1 9958 1 ROTIPHORESE PROfessional runVIEW Base MINI 1 o 1x blue light illuminator adjustable to 2 different sizes including power cord o 1x special lid with spectral emission filter and extractor fan within its viewing pane for dyes with green and red fluorescence o Instruction manual The lid is sui...

Page 3: ...able for dyes with green fluorescence e g the non toxic dye SYBR Green and red fluorescence e g ethidium bromide or the non toxic dye ROTI Gelstain Red Art No 0984 The blue light illuminator provides instant time saving visualisation of DNA RNA band migration No UV safety equipment required for blue light illumination For the ROTIPHORESE PROfessional III unit gel trays of 15 x 7 15 x 10 and 15 x 1...

Page 4: ...late with wet hands 7 Do not connect to ground any of the power outputs or the buffer within the electrophoresis tank the power outputs should be only connected to an insulated electrophoresis tank equipped with a safety cover 8 Do not connect any power supplies in series or in parallel 9 Never open the back plate nor remove the cover otherwise an electric shock may result Repairs should only be m...

Page 5: ...are partially shrouded for protection However when cleaning the main tank do not use cleaning brushes in the electrode area Ensure that the connectors are clean and dry before usage or storage RNAse Decontamination Clean the units with a mild detergent as described above Wash with 3 hydrogen peroxide H2O2 for 10 minutes Rinse with 0 1 DEPC diethyl pyrocarbonate treated distilled water Caution DEPC...

Page 6: ...e Use to alternatebetween Voltage Current and Time Use or to adjust the value to the desired setting b Operation Mode Press to start electrophoresis The LED light next to the Start Stop button will light up to indicate the unit is in operation Use to monitor the remaining time and changes in current and voltage c Blue light There are two modes of blue light illumination for visualisation of nuclei...

Page 7: ...he DNA loading and staining reagents ROTI Load DNAstain1 2 or 3 containing the non toxic fluorescent dye EvaGreen see below 5 Select your settings accordingly In order to run the system at constant voltage switch the mode button to the Voltage setting and alter the value to the desired setting as described in Set Up Mode the Volt LED will be illuminated by this stage 6 Use the same principle to ru...

Page 8: ... shielding the unit from light by a dark cloth or paper 2 In order to conserve the blue light lamp the light will be there for 10 seconds only and then switch off again by its own Press the button for 3 seconds to switch constant light on Using runVIEW for DNA recovery 1 Cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied before transferring the blue lig...

Page 9: ...oresis the application of current through a gel leads to a build up of heat resulting in the accumulation of condensation within the runVIEW lid viewing pane Excessive levels of condensation impair visualisation of the nucleic acid bands within the gel Condensation may be cleared by using the fan extractor in runVIEW lid G At the End of the Run Turn the power supply settings to zero turn off mains...

Page 10: ...or ROTIPHORESE PROfessional II electrophoresis unit Special lid for runVIEW system green and red fluorescence 3680 1 Power Supply Roth MINI 2907 1 Power Supply EV1450 CP56 1 Further accessories see Professional II unit Art No 2799 1 K Additional Items and Reagents Levelling table N854 Agaroses Standard 3810 NEEO ultra quality 2267 Agarose Tablets HP67 Broad Range for all fragment lengths T846 GTQ ...

Page 11: ...suitable for all PROfessional gel trays up to a size of 26 x 32 cm W x L When using gel trays up to a size of 10 x 10 cm 2 gels can be poured simultaneously PROfessional I and II The level bubble allows an optimal levelling of the gels Additional sealing is not necessary L Troubleshooting and Maintenance Each runVIEW system uses all solid state components and should require no maintenance or recal...

Page 12: ...ircuit is broken in the electrophoresis system Check the connections to the power supply and within electrophoresis system to make sure the connection is intact check the electrodes to make sure they are intact Close the circuit by reconnecting the cables Press START STOP to restart the run High resistance due to tape left on a pre cast gel incorrect buffer concentration or insufficient buffer vol...

Page 13: ...2020 Complete electrophoresis system ROTIPHORESE PROfessional runVIEW 4849 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base Suitable for Unit PROfessional III 4850 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base MINI 1 Suitable for Unit PROfessional I 9958 1 Electrophoresis Station ROTIPHORESE PROfessional runVIEW Base MINI 2 Suitable for Unit PROfessional II 9961 1 ...

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