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Raptor Photonics OWL 640 M, User Manual

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Raptor Photonics OWL 640 M User Manual Download Page 2

OW1.7-VS-CL-LP-640/USER MANUAL/08-20/REV1.4 

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CONTENT 

 

 

CONTENT........................................................................................................................................... 2 

1.  INTRODUCTION ...................................................................................................................... 3 
2.  SPECIFICATION ....................................................................................................................... 4 
3.   GETTING STARTED .................................................................................................................. 6 
4.   EPIX XCAP .............................................................................................................................. 8 
5.  MICRO-MANAGER ................................................................................................................ 18 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Summary of Contents for OWL 640 M

Page 1: ...OWL 640 M Model OW1 7 VS CL LP 640 USER MANUAL...

Page 2: ...OW1 7 VS CL LP 640 USER MANUAL 08 20 REV1 4 2 CONTENT CONTENT 2 1 INTRODUCTION 3 2 SPECIFICATION 4 3 GETTING STARTED 6 4 EPIX XCAP 8 5 MICRO MANAGER 18...

Page 3: ...local agent To clean the sensor window gently wipe the face of the sensor window with a small amount of optical grade isopropyl alcohol and lens paper Apply forced air again to remove any loose parti...

Page 4: ...LG Low Gain HG High Gain LG 190 electrons 174 electrons typical HG 50 electrons 38 electrons typical Quantum Efficiency 80 1 55 m Full Well Capacity LG 650ke HG 9ke Pixel Operability 99 Digital Output...

Page 5: ...OW1 7 VS CL LP 640 USER MANUAL 08 20 REV1 4 5 2 2 Mechanical Outline 3D drawings STEP are available upon request...

Page 6: ...dard C Mount that should easily screw onto any microscope port 3 3 Mounting to a tripod or optical table The camera has a 20 BSW Whitworth threaded hole to mount to a tripod or an optical table The im...

Page 7: ...connect the camera to the computer Connect the other end of the Camera Link cable to the camera Carefully thread the C mount lens onto the camera s lens ring rotating the lens in a clockwise directio...

Page 8: ...the latest cameras and latest PIXCI imaging boards Open the downloaded file when complete and follow the onscreen instructions Be sure to accept the board driver installation 4 2 Operating your camer...

Page 9: ...lect your chosen camera For example for the Owl 640 it should read EPIX PIXCI E8DB Raptor Photonics OWL CL 640 If not you need to select a different camera 8 Check that the camera is communicating see...

Page 10: ...gure 5 Image Capture 12 You should now see an image On the bottom of the screen you should see something similar to the following Frame 0 Size 640 x 512 Resize 0 7x0 7 Video 26 0fps Capture 26 0 fps D...

Page 11: ...ndow of the EPIX software 4 4 Exposure Click on the Gain Tab There is an option for Auto Level Control ALC When Auto Level Control is unchecked you can manually adjust the exposure and digital gain us...

Page 12: ...re 8 Trigger Tab 4 6 Auto On this Tab you can select the parameters for the Automatic Level Control Including the speed and spread of signal taken into account In this mode the FPGA of the camera will...

Page 13: ...osition By default this will be set to account for the whole image Edit the values of Offset Width and Height to place the Auto Level Control ROI over the image Figure 10 Auto ROI Tab Figure 11 Active...

Page 14: ...e exposure but the box becomes too dark to see any detail This is an extreme example used to illustrate this feature 4 8 NUC On this tab you can select the parameters for the on board Non Uniformity C...

Page 15: ...EV1 4 15 Figure 14 NUC Off Figure 15 NUC On 4 9 TEC Thermoelectric Cooling TEC Use this tab to set and read back the sensor temperature The optimum temperature should be set on start up for the OWL 64...

Page 16: ...ch that dark areas in the image will appear bright by checking the video invert box Figure 17 Camera Information Output video can be digitally processed to provide image sharpening by checking the Act...

Page 17: ...best display of the RAW image data from the camera 1 Go to the Modify Contrast Modification menu Figure 19 2 Select Stretch Contrast Histogram Percentile Endpoints and Preview Figure 20 Stretch Contr...

Page 18: ...ackage available for Windows Mac and Linux it is easy to install and configure right out of the box with built in functionality and user interface for most common tasks performed in a Life Science lab...

Page 19: ...by going to the downloads section of their website www Micro Manager org You should select the latest nightly build for your operating system found in the Download Nightly Builds section This will ope...

Page 20: ...Micro Manager for the first time you will see the window shown in Figure 22 You need to make sure none is selected then click Ok Figure 22 Hardware Configuration 1 Start the Hardware Configuration Wiz...

Page 21: ...ver in the RaptorEPIX folder shown in Figure 24 now click Next Figure 24 Add a New Device 3 Continue to click Next until you are faced with the screen shown in Figure 24 4 Rename and save new configur...

Page 22: ...P 640 USER MANUAL 08 20 REV1 4 22 Next time you open Micro Manager you can simply select the saved Raptor Owl 640 cfg from the drop down menu figure 26 then click Ok Figure 26 Selecting Saved Camera C...

Page 23: ...USER MANUAL 08 20 REV1 4 23 5 3 Operating your camera using Micro Manager Figure 27 Opening Screen 1 Click on snap Figure 27 for Micro Manager to open the viewing window as shown in Figure 28 Figure...

Page 24: ...mera settings All of the camera parameters are controlled within the Device Property Browser this can be accessed by selecting Tools from the menu bar then Device Property Browser Figure 29 Camera Con...

Page 25: ...OW1 7 VS CL LP 640 USER MANUAL 08 20 REV1 4 25 CORPORATE HEADQUARTERS Raptor Photonics LTD Willowbank Business Park Larne Co Antrim BT40 2SF Northern Ireland PH 44 2828 270141 www raptorphotonics com...

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