6
4. Wipe any excess liquid from the outside of the tip
without touching the orifice.
5. To dispense, rest the end of the tip against the
vessel wall and press the plunger to the first stop.
Hold this position a few seconds, or long enough
for the liquid column to reach equilibrium again.
6. Remove the tip from the receiving vessel without
blowing out the remaining liquid.
7. Return excess sample in the tip to the original
sample container, if desired. Discard the used tip.
Pipetting Liquids of Varying Density
Pipet-Lite lets you compensate for solutions of densi-
ty much different from water, by setting the volume
slightly higher or lower than that required. The com-
pensation amount must be determined empirically.
For example, if pipetting 10 µL of CsCl solution, you
determine that the volume delivered is actually 8.5 µL
(
≥
5 samples). Change the volume setting to 11.8 µL
and repeat the measurements. If the volumes deliv-
ered are still not close enough to 10 µL, make anoth-
er slight volume adjustment until the measurements
are as desired.
Temperature Considerations
Warm or cold liquids can be measured with good pre-
cision by using a consistent pipetting rhythm. This will
help minimize any differences in heating or cooling
effects within the pipette.
Use a new tip each time for best accuracy and preci-
sion when measuring samples with temperatures
greatly different from ambient, and do not pre-rinse.
As with any air-displacement pipette, you will get best
results if there is no delay between picking up the
sample and dispensing it.
Autoclaving
Autoclavable parts of Pipet-Lite are the shaft and the tip
ejector: 121°C, 1 bar, 15-20 minutes.
Do not autoclave the complete pipette or any
parts other than the shaft and the tip ejector.
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