Quidel Sofia Influenza A+B Quick Reference Instructions Download Page 3

 

WALK AWAY/READ NOW Modes 

Refer to the Sofia User Manual

.

 

Sofia may be set to two different modes (WALK AWAY and READ NOW). The procedures for each mode are described below. 

     

 

In WALK AWAY Mode, the user 

immediately

 inserts the Test Cassette into Sofia. 

Sofia will automatically time the test development, and the results will be 
displayed in 15 minutes. 

 

The user places the Test Cassette onto the counter or bench top for 15 minutes (outside of 
Sofia). The user manually times this development step. The Test Cassette MUST remain on 
the bench for 15 minutes to get an accurate result. Then, the user inserts the Test Cassette 
into Sofia. In READ NOW Mode, Sofia will scan and display the test result in 1 minute. 

Note:

 

Results will remain stable for an additional 15 minutes after the recommended 
development time of 15 minutes. 

 

 

 

 

RUN TEST 

1.

 

Input the User ID with the barcode scanner or enter 
the data using the key pad. 

NOTE

If you scan the wrong barcode, use the Arrow 

Keys on Sofia to re-highlight the field and simply 
rescan using the correct barcode. The previous one will 
be overwritten with the right barcode.

 

 

3.

 

Press Start Test and the Sofia drawer will 
open. 
 
 

4.

 

Verify that the correct mode (WALK 
AWAY or READ NOW) has been selected. 
Insert the Test Cassette into the drawer. 
Then gently close the drawer. 

 

2.

 

Input the Patient ID or Order # with the barcode 
scanner or enter the data using the key pad. 

 

5.

 

Sofia will start automatically and display the progress. In WALK AWAY 
Mode, the test results will be displayed in  
approximately 15 minutes. In READ NOW  
Mode, the test results will be displayed in  
1 minute. See Sofia Interpretation of  
Results section. 

Allow the test to develop for the FULL 15 minutes BEFORE placing it into Sofia. 

 WALK AWAY Mode 

 READ NOW Mode 

Using Sofia 

Summary of Contents for Sofia Influenza A+B

Page 1: ... bulb b Still squeezing place the Pipette tip into the sample c With the Pipette tip still in the sample release pressure on bulb to fill the Pipette Firmly squeeze the top bulb to empty the contents of the Small Clear 120 µL Fixed Volume Pipette into the Test Cassette sample well Extra liquid in the overflow bulb is OK NOTE The Fixed Volume Pipette is designed to collect and dispense the correct ...

Page 2: ...he collection cup Firmly squeeze the top bulb to empty the contents of the Large Pink 250 µL Fixed Volume Pipette into the Reagent Tube Extra liquid in the overflow bulb is OK NOTE Once the sample is added to the Reagent Tube vigorously mix prior to adding the sample to the Test Cassette NOTE The Fixed Volume Pipette is designed to collect and dispense the correct amount of patient sample Discard ...

Page 3: ...mended development time of 15 minutes RUN TEST 1 Input the User ID with the barcode scanner or enter the data using the key pad NOTE If you scan the wrong barcode use the Arrow Keys on Sofia to re highlight the field and simply rescan using the correct barcode The previous one will be overwritten with the right barcode 3 Press Start Test and the Sofia drawer will open 4 Verify that the correct mod...

Page 4: ...Flu B Positive Procedural Control Valid Positive Test for both Flu A and Flu B influenza A and B antigen present Flu A Negative Flu B Negative Procedural Control Valid Negative Test for Flu A and Flu B no antigen detected Flu A Invalid Flu B Invalid Procedural Control Invalid Result Invalid From the main menu select Run QC Follow the prompts on the screen Scan the QC Card located on the kit box So...

Page 5: ...in stable for an additional 15 minutes after the recommended development time of 15 minutes RUN TEST 1 Input the User ID with the integrated barcode scanner or manually enter the data using the key pad NOTE If you scan the wrong barcode re highlight the field using the touchscreen on Sofia 2 The previous one will be overwritten with the right barcode 3 Verify that the correct mode WALK AWAY or REA...

Page 6: ...in the rapid differential diagnosis of acute influenza A and influenza B viral infections The test is not intended to detect influenza C antigens A negative test is presumptive and it is recommended these results be confirmed by viral culture or an FDA cleared influenza A and B molecular assay Negative results do not preclude influenza virus infections and should not be used as the sole basis for ...

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