Qiagen QIAcuity 911000, User Manual

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QIAcuity User Manual 06/2022 206
1D Scatterplot tab for absolute quantification
The 1D Scatterplot tab shows one 1D Scatterplot view for each analyzed target or channel. If there
are more than one scatterplot views, they are separated with a horizontal line. The 1D Scatterplot
views are sorted by channel position in imaging (“Green”, “Yellow”, “Orange”, “Red”, “Crimson”).
The title of a 1D Scatterplot view shows the related channel name including the dot channel color
indicator, and if defined also the target name. If more than one well is selected also the number of
wells is shown.
Thus, a 1D Scatterplot view has two axes. The x-axis represents the analyzed partitions, while the
y-axis represents the relative fluorescence intensity of each partition.
A 1D Scatterplot view concentrates the diagrams for each selected well in a horizontal way
separated by a vertical line (column indicator). For each well diagram a header is shown indicating
the well coordination on the plate. A red line represents the current threshold fluorescence intensity
value (decimal value) that is used to distinguish between positive and negative partitions.
Fluorescence values below the threshold are shown in grey, above the threshold in blue color.
In one 1D Scatterplot at maximum twelve well diagrams are shown on the page. The well diagrams
are scaled according to the number of wells shown on one page. If there are more than seven
selected wells, a horizontal scrollbar below the 1D Scatterplot is shown to the user to view the other
wells.
To view an additional toolbar that enables you to perform actions related to the diagram, such as
downloading the plot, zoom-in, zoom-out, hold the pointer over the diagram. For more details about
the toolbar, refer to Diagram options
.
The user can change the range of the y-axis by adjusting the value in the field Max value for the
y-axis (RFU) and clicking
Save
. For resetting the y-axis to the default range, press the
Back to default
button. The view of the diagram is adapted accordingly.
Note : For RFU values below 60 it is recommended to increase the exposure time of corresponding
channel/s and for RFU values below 150 it is recommended to decrease the exposure time of
corresponding channel/s.