Qiagen PyroMark Q96 ID, User Manual

Page: 35 / 126

Operating Procedures
PyroMark Q96 ID User Manual 01/2016 5-3
Optional
If desired, enter a note about the assay and set up the
variable positions in the “Variable Positions” tab.
If creating a CpG assay, it is recommended that bisulfite
treatment controls are added. In the sequence before
bisulfite treatment, check if the suggested bisulfite controls
are
C s converted to T s (read as G s and A s in a reverse
assay) and are suitable for controls or not.
To add a control, left-click a bold, orange T or A in the
histogram, preferable at the beginning of the sequence.
If applicable, during the assay validation, edit the analysis
parameters.
5.3.2 Setting up a run
1. In the shortcut browser, right-click the folder you want to
place the run file in, and select “New Run” from the
context menu.
Note : To add a shortcut to a folder in the shortcut
browser, click “Add Folder Shortcut”.
2. Click in the toolbar to enter a run name and save the
file in the desired folder.
3. In the “Instrument Parameters” drop-down list, select the
instrument parameters file that corresponds to the
PyroMark Q96 Cartridge to be used.
4. Optional : Enter the “Reagent ID”, “Plate ID”, plate “Bar
code”, or a “Run note”.
5. Add an assay to each well used, e.g., drag an assay
from the shortcut browser to a well or a selection of
wells. A well is colored according to the assay loaded
into the well. Alternatively, right-click a well or selection
of wells and select “Load Assay”.