1. .
Quick Protocol
Instructions for Use of Product AS1831.
Maxwell
®
RSC Enviro Total Nucleic Acid Kit
P R O M E G A C O R P O R AT I O N
•
2 8 0 0 W O O D S H O L L O W R O A D
•
M A D I S O N , W I 5 3 7 1 1 - 5 3 9 9 U S A
•
T E L E P H O N E 6 0 8 - 2 7 4 - 4 3 3 0
w w w . p r o m e g a . c o m
•
© 2 0 2 1 P R O M E G A C O R P O R AT I O N
•
A L L R I G H T S R E S E R V E D
•
P R I C E S A N D S P E C I F I C AT I O N S S U B J E C T T O C H A N G E W I T H O U T P R I O R N O T I C E
•
P R I N T E D I N U S A 0 7 / 2 1
•
PA R T # F B 2 3 7
11339T
A
1
2
3
4
5
6
7
8
1. Binding Buffer
2. Magnetic Particles
1. Preprocessed sample
8. CSC Plunger
3. Wash Solution
4. Wash Solution
5. Wash Solution
6. Wash Solution
7. Empty
8. Empty
Well Content User Adds:
Additional protocol information in Technical Manual #TM663, available online at:
www.promega.com
Total Nucleic Acid Extraction and Clean-Up on Maxwell
®
Instrument
Cartridge Preparation
1. Place the cartridge to be used in the Deck Tray(s) with well #1 (the largest well)
facing away from the elution tube.
2. Press down on the cartridge to snap it into position. Carefully peel back the seal
so that all plastic comes off the top of the cartridge. Ensure that all sealing tape
and any residual adhesive are removed before placing cartridges in the
instrument.
3. Place a plunger in well #8 of each cartridge. Well #8 is the well closest to the
elution tube.
4. Place an empty elution tube in the elution tube position for each cartridge. Add 80μl of Nuclease-Free
Water to the bottom of each elution tube.
5. Add 150μl of Binding Buffer 1 and 50μl of Binding Buffer 2 to the 0.5ml of liquid eluted in Step 18.
6. Load the entire volume of mixture to well #1 (the largest well).
Maxwell
®
Instrument Run
Follow the instrument run instructions in the
Maxwell
®
RSC Enviro Total Nucleic Acid Kit Technical Manual
, #TM663.
Figure 2. Maxwell
®
RSC Cartridge. Sample concentrate is
added to well #1, plunger added to well #8.
