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Kryo250

Operators' Manual

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Summary of Contents for Kryo250

Page 1: ...Kryo250 Operators Manual...

Page 2: ...2004 Planer plc Planer plc 110 Windmill Road Sunbury on Thames Middlesex U K TW16 7HD Telephone 44 0 1932 755000 Facsimile 44 0 1932 755001 Kryo 250 Operators Manual MA009478 Issue 1 0 July 2004...

Page 3: ...should be routinely kept All equipment supplied by Planer plc is subject to a Warranty Agreement and Limited Liability In addition software products are not sold but are licensed for use and are subje...

Page 4: ...nstructions could result in damage to equipment or samples This indicates first aid advice Symbols Used on the Equipment Caution Refer to this manual and accompanying documentation Mains supply ON COO...

Page 5: ...ecifications see Appendix A may result in the protection offered by the equipment being impaired Avoid nitrogen build up anywhere liquid nitrogen is used must be well ventilated to reduce the risk of...

Page 6: ...may get cold enough to cause frostbite The dewar must be depressurised before the pump can be removed This is achieved by opening the pressure relief valve mounted on the side of the pump which seals...

Page 7: ...le The AUX socket on the Controller is to be used only for calibration by qualified service personnel It must not be connected to any other equipment The Sample PRT socket on the chamber s rear panel...

Page 8: ...le Fit the plastic covers and plugs provided to all unused connectors Immediately before touching the sample PRT touch an earthed part such as the metal surface at the rear of the Controller Do not to...

Page 9: ...r Rear Panel 2 2 Controller Front Panel 2 1 Chamber Front Panel 2 1 Operating the Kryo250 Chapter 2 1 8 Loading Printer Paper 1 7 Removing the Pump 1 7 Testing the Dewar 1 6 Fitting the Pump 1 5 Filli...

Page 10: ...ses A 2 Chamber Specifications A 1 Controller Specifications A 1 System Specifications A 1 Specifications Appendix A 3 4 Returning for Service 3 3 No Response from the System 3 3 The Chamber will not...

Page 11: ...Top View In the above diagram the chamber is shown in its vertical position The chamber can be placed horizontally if preferred Installing the System 1 1 Hose connection to liquid nitrogen RS232 port...

Page 12: ...not connect until instructed to do so in the manual Power to LNP4 pump if used Power from chamber to controller Manual override Reset button AUX socket RS232 to PC 37 way D Type connection to chamber...

Page 13: ...a the 9 way plug always use a fully screened cable no longer than 2m If connecting to a PC connect the 9 way D type plug on the rear of the Controller to an RS232 port on the PC using the one to one c...

Page 14: ...ed into the controller using a PC connection Please contact your supplier for details If the hose is not already fitted to the Chamber wrap the male hose thread with PTFE tap and fix to the LN2 input...

Page 15: ...ure that the fibre washer is fitted to this joint Tighten securely with a spanner Filling the Dewar When refilling a dewar that already contains liquid nitrogen you must first depressurise it and remo...

Page 16: ...ently In one smooth and continuous action insert the pump evenly into the neck of the dewar until the microswitch plunger operates on the neck ring It is normal for nitrogen gas to issue from the pres...

Page 17: ...connect the mains supply at the wall socket open the red pressure relief valve and wait for the flow of gas to stop Check for liquid and gas leaks Removing the Pump You will need to remove the pump wh...

Page 18: ...ion The end of the pump may be cold enough to cause frostbite Place the pump in a safe position on its side or suspended vertically Remove frost using a hot air gun Do not overheat as the plastic part...

Page 19: ...Chapter 2 Operating the Kryo250 Chamber Front Panel Operating the Kryo250 2 1 Indicates when chamber is being cooled Indicates when chamber is being heated Mains on off switch...

Page 20: ...without a working sample PRT but the chamber PRT must be in full working order indicates a stuck button S indicates that samples are ready for seeding if this feature is selected Sample temperature bu...

Page 21: ...0 2 3 RS232 Connection to PC Auxilliary connector for service calibration only Reset the controller Press to force an increase in temperature Press to force a reduction in temperature Mains inlet 37 W...

Page 22: ...to pressurise exceeds five minutes check that there are no gas leaks and that the dewar is not overfilled or empty v If the dewar has not been recently filled test its weight to judge whether it conta...

Page 23: ...he end of the run is signalled by long beeps and short flashes Press the STOP button on the front of the Controller v Remove the samples and furniture if appropriate Stopping a Run At any time you can...

Page 24: ...the chamber is back at ambient temperature However if the equipment is to remain unused for longer than a working day also turn off the liquid nitrogen tap or in the case of a Planer dewar depressuris...

Page 25: ...l continue to monitor the chamber temperatures to ensure that it can resume the profile upon return of mains power If power resumes within 1 minute the run will continue If power is removed for more t...

Page 26: ...2 8 Operating the Kryo250...

Page 27: ...ing water and detergent or IPA alcohol v Check all nitrogen fittings periodically for any signs of leaks or damage v Check all mains cables and interconnecting cables for signs of damage The Kryo250 S...

Page 28: ...e program will probably finish correctly v If the solenoid valve has stopped for more than five minutes estimate the temperature of the samples by estimating the amount of time that the program has be...

Page 29: ...y ice if using a Planer pump The Chamber will not Heat v Check the fuses v Check that the Chamber did not overheat wait until the thermal cut out resets No Response from the System v Check the mains s...

Page 30: ...need to be sent back to Planer plc for repair or if the system is to be inspected maintained or repaired on site by Planer plc personnel the Safety Declaration described in Appendix B Safety Declarati...

Page 31: ...50 C Storage temperature 0 3 0 005 x TM C where TM is the magnitude of the temperature Accuracy Controller Specifications 32 Steps per profile 1 Number of profiles 3 key membrane keypad Keypad 3 5 dig...

Page 32: ...weight kg 1 7 Kryo Chamber Note The Kryo250 systems are designed to be connected to an Installation Category over voltage category II mains supply as defined in IEC 664 Fuses Chamber T 3A L 250V 1 25...

Page 33: ...dations support the issue of a certificate statement of decontamination prior to submitting the equipment for servicing The declaration overleaf must be completed signed by a competent authority and r...

Page 34: ...cleaned as necessary to render it safe to handle and service It does NOT contain any Biohazard or Toxic material Serial Nos Equipment Type Customer Order No Service Order The preceding notes on page B...

Page 35: ......

Page 36: ...MA009478 Kryo250 Operators Manual...

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