PerkinElmer Wallac 1420, User Manual

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CW-Lamp energy
In fluorescence polarization constant voltage mode is used and the light source is driven
with high power. The software corrects for changes in energy of the CW-lamp to ensure that
results are calculated for a constant excitation energy.
For fluorescence polarization you
should always use maximum power, 65535 .
CW-Lamp filter
You can select the CW-lamp excitation filter from the drop-down list of available filters.
See the chapter on Filters for more information about how to define filters. Factory pre-set
CW-lamp filter names begin with a letter indicating the technology for which they are to be
used: F is for fluorometry. After the letter comes the wavelength.
Polarizer aperture
There are two sizes for the aperture of the S-plane polarizer in the excitation light path. You
can select by option buttons whether this is Small (for high-density plates) or Normal (96 or
384-well plates).
Emission filter
You can select the emission filter from the drop-down list of available filters. See the
chapter on Filters for more information about how to define filters. Factory preset emission
filter names begin with a letter indicating the technology for which they are to be used: F is
for fluorometry. After the letter comes the wavelength.
Emission aperture
In front of the photomultiplier tube there is a 4-position aperture slide, which has three
different apertures and a shutter position. The normal aperture, which is circular with a
diameter of about 4 mm, should be used for fluorescence polarization.
Counting time
This parameter determines how long the excitation continues. The following times are
recommended for 96 and 384-well plates:
Concentration (nM) Measuring time (s)
2 or more 0.1
1 0.2
0.5 0.3
0.1 1.0