PerkinElmer Panthera-Puncher 9, User Manual

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Troubleshooting
Issue Solution
I am getting error messages related to punch heads or
warning that disks are not detected.
Cleaning the punch heads may be necessary.
I am getting a lot of problems with disks jumping to
wrong positions during the dry season.
Static electricity may cause issues when the air humidity
is low. To reduce the effects of static electricity, the
instrument is equipped with an ionizer. If this does not
provide sufficient help, you can try the following provided
the assay you are running does not prevent these:
• Humidify the air in the lab
• Reduce the use of synthetic fibers in the operator's
clothing
• Use an antistatic gun or a similar instrument to
reduce the plate charge before starting the punching
process
• Before unloading the plates from the instrument, wet
the plates with a suitable substance (depending on
your assay)
• Use an external ionizer in the lab
• Ground the user
When using Intellipunch , I get a certain number of disks
from a single spot. Once these have been punched, the
instrument suggests that one more disk be punched from
that same spot. Is this normal?
This is due to the inaccuracy of pixel size calibration,
which ensures that the disks fit. In some situations, a
disk can be a pixel smaller than was calculated,thus
prompting the instrument to punch one more disk.
Depending on your configuration, it can also be due to
the blood border and disk border settings.
Panthera disables the plate and issues a warning, stating
that local settings need to be created.
When working with Specimen Gate, the analyte settings
must be the same in Specimen Gate and in standalone
mode. If the settings do not match, Panthera issues a
warning. If no local settings are found for an analyte,
Panthera will disable the plate and issue a warning
stating that local settings need to be created. Contact
your local PerkinElmer representative.
The punch locations are repeatedly lost and found on the
screen.
In situations where external lighting conditions are
changing, the Panthera-Puncher 9 may repeatedly find
and lose punching locations. This can be corrected by
adjusting the lighting in the room and in the instrument.
To adjust the lighting conditions, contact your local
PerkinElmer representative.
Panthera-Puncher
™
9 User Manual
Troubleshooting
32