PerkinElmer Panthera-Puncher 9 User Manual Download Page 13

Page: 13 / 42

User group

Access

Puncher Administrator

Has all the access rights to the Panthera Manager

software, and is granted access to service software for

reading log files. When a user in this group opens the

service software, a message informs them that the only

feature available to them is the log reader. Press

clear the message, and the service software starts. Please

note it may take the software some time to load the log

file.

Puncher Service

This user group is only for certified PerkinElmer service

personnel.

The Panthera-Puncher 9 comes programmed with three different users by default (Panthera User, Panthera Admin and

Panthera Service), belonging respectively to their corresponding Windows User Group. The service personnel have the

access rights to create more users. When creating a new user, they will need to be added to one of the three different

Windows User groups on the PC.

Note:

If a user with insufficient Windows user rights tries to open the Panthera software, the configuration files are

virtualized. If the user with insufficient rights is later granted rights to use the Panthera software, the virtual files must

be removed or they will cause issues. For example, the punch head cleaning performed by a user with virtual files will

not be visible to other users.
Once you are logged in, start the Panthera Manager software by double-clicking its icon on the desktop.
The software splash screen is displayed for a few seconds while the instrument initializes. After the initialization, the

Plates

view appears on the screen.

Note:

While the software is starting and the splash screen shown, the sample plane needs to be free of any cards or

papers. The camera will calibrate the control level for recognizing the cards while the software starts up.
To close the Panthera Manager software, press the

Quit

button in the

Maintenance

view (for more details on this,

please refer to

System information

on page 16).

If the instrument contains loaded plates while closing the software, it will warn you that all the plate related data will

be lost if the plates are not unloaded before the software closes. Even if the plates are empty, do not close the software

with plates loaded. This may cause the plates to become unusable.

Loading and setting up the plates

Press the

Eject

button in the

Plates

view and open the hatch. Once the hatch is open, the plate track comes out. The

plate track contains 9 plate positions. All 9 positions are suitable for standard 96-well plates.

Warning:

The plate track comes out of the instrument approximately 10 cm (4 inches) when ejected.

Positions 7 through 9 contain removable adapters that, when removed, can hold deep well plates. If you are not using

deep well plates and instead want to use plates of regular height in these positions, remember to use the adapters.
The plate should be oriented with the A1 well in the back left corner. When loading the plates, make sure you position

them between the guide pins.
Place the plate barcodes on the long side of the plate (facing inside the instrument) in order for the instrument to read

them automatically.
The instrument will detect empty plate positions by detecting the "empty" barcode printed on the track at each plate

position.
Press

Done

when all the plates are loaded onto the plate track. The plate track will go back inside the instrument.

Wait

until the plate track is completely inside the instrument before closing the hatch.

The instrument will start to

scan the plate barcodes once the hatch has closed.
When the instrument has read the plate barcodes, it displays a plate map for each plate in the

Plates

view. If the

instrument encounters an issue with a plate (e.g. no barcode or unknown analyte), it will mark the corresponding plate

map with a red outline and display an exclamation mark next to the field where the plate barcode should be. You can