PerkinElmer OilExpress 4, User Manual

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100 . OilExpress 4 System User's Guide
11. Place the flow cell on the bench (Figure 57).
Do not to stretch the tubing between the FCM and the upper port of the flow cell. You
may find it easier to disconnect the tubing from the FCM.
Figure 57 Flow cell removed from sample shuttle
12. Unscrew (counter-clockwise) the blue connectors and pull gently to remove the tubing
from the upper and lower ports of the flow cell.
NOTE: Take care not to lose the ferrule from the end of the tubing. Check that the ferrule
has not remained inside the metal front of the flow cell. The narrow end of the
ferrule points inwards, towards the connector (Figure 58).
Figure 58 Tubing showing blue nut connector and ferrule
13. Perform any maintenance on the cell, or locate a new cell.
To replace the flow cell in the system:
1. Ensure that the blue nut connector and the ferrule are correctly fitted to the end of the
tubing.
The narrow end of the ferrule should point inwards, as shown in Figure 58.
NOTE: If the threads on the connector are damaged, replace it with a new connector and
ferrule to prevent leaking. A poor seal at the lower fitting can cause air to be
introduced into the flow cell with the sample, which can affect the quality of the
analysis. A poor seal at the upper fitting could lead to problems in effectively cleaning
the cell between samples.
2. Hold the tubing in one hand and push the end of the tubing into the upper port of the
flow cell, so that the ferrule is pushed up to the inside of the cell.
Lower (intlet)
port
Upper (outlet)
port