PerkinElmer LAMBDA 265 User Manual Download Page 3

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Contents . 3

Table of Contents

Introduction ...................................................................................... 5

Overview ............................................................................................... 6

About This Manual .................................................................................. 7

Basic Theory .......................................................................................... 8

PDA UV-Visible Spectrophotometer .................................................... 9

Safety Summary ................................................................................... 10

Instrument Shipping Kit ........................................................................ 13

Conventions Used in this Manual ............................................................ 14

Notes, cautions and warnings .......................................................... 14

Note, attention et avertissement ..................................................... 14

Safety Practices .............................................................................. 19

Overview ............................................................................................. 20

Precautions .......................................................................................... 21

General Operating Conditions ................................................................ 22

Electrical Safety .................................................................................... 23

EMC Compliance ................................................................................... 25

Warning Labels .................................................................................... 26

WEEE Instructions for PerkinElmer Products ........................................... 27

Decontamination ............................................................................ 28

Cleaning the Instrument ................................................................. 28

Fuses .................................................................................................. 29

Lambda 265 Installation ................................................................. 31

Preparing the Laboratory ....................................................................... 32

Installation ........................................................................................... 34

UV Lab Software Installation ......................................................... 35

How to Install the UV Lab Software ........................................................ 36

Interface Setup ............................................................................... 41

Setting USB Serial Ports ........................................................................ 46

Operating Procedure ....................................................................... 51

Procedure ............................................................................................ 52

Accessories ...................................................................................... 54

Accessories for the Lambda 265 ............................................................. 55

Single Cell Holder ........................................................................... 55

Water Jacketed Single Cell Holder.................................................... 56

Variable Pathlength Holder .............................................................. 56

Advanced Transmission Holder ........................................................ 57

Reflectance Module ........................................................................ 57

Peltier Controlled Single Cell, With Peltier Controller .......................... 57

8 Position Multi Cell Holder ............................................................. 58

Water Jacketed 8 Position Multi Cell Holder ...................................... 58

Autosampler .................................................................................. 58

Nano Stick ..................................................................................... 59

Magnetic Stirrer Assembly ............................................................... 59

Index .................................................................................................. 60

Summary of Contents for LAMBDA 265

Page 1: ...LAMBDA 265 Users Guide UV SPECTROMETERS ...

Page 2: ...ted to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication...

Page 3: ...ontamination 28 Cleaning the Instrument 28 Fuses 29 Lambda 265 Installation 31 Preparing the Laboratory 32 Installation 34 UV Lab Software Installation 35 How to Install the UV Lab Software 36 Interface Setup 41 Setting USB Serial Ports 46 Operating Procedure 51 Procedure 52 Accessories 54 Accessories for the Lambda 265 55 Single Cell Holder 55 Water Jacketed Single Cell Holder 56 Variable Pathlen...

Page 4: ...4 Lambda 265 Users Guide ...

Page 5: ...Introduction 5 Introduction ...

Page 6: ... for installing and operating the Lambda 265 UV Visible Spectrophotometer and UV Lab software It also introduces and describes how to use basic features of the UV Lab software For more detailed information on software features including kinetics bio analysis color analysis and MCA refer to the UV Lab software user guide ...

Page 7: ... Installation Information on installing and re installing your instrument should you ever need to move your system is provided Chapter 4 UV Lab Software Installation The complete UV Lab software installation procedures for the Lambda 265 is in this chapter Chapter 5 Interface Setup Interface procedures for the Lambda 265 Chapter 6 Operating Procedure This chapter contains information on operating ...

Page 8: ...varies depending on the wavelength of the radiation Data from absorption spectra can be used for qualitative and quantitative analysis A molecule remains in its ground state when it is stable but it can transition to an excited state when light energy is absorbed This is termed absorption Figure 2 When the excited molecule returns to the ground state it emits heat radiation fluorescence or phospho...

Page 9: ...t is widely applied to quantitative analysis Figure 4 Transmittance and Absorbance PDA UV Visible Spectrophotometer The PDA Spectrophotometer has a multi channel detector controlled by a microprocessor which collects spectral data for several wavelengths simultaneously In contrast a PMT Spectrophotometer has a single channel detector which collects data for each specific wavelength in turn Therefo...

Page 10: ...indications de danger Warning When supplying power to this equipment connect the accessory 3 pin power cord to a 3 pin grounded power outlet If a grounded 3 pin outlet is not available use a conversion adapter and ground the green wire If power is supplied without grounding the equipment there is a risk of receiving a fatal electric shock and equipment damage Lors de l alimentation de l équipement...

Page 11: ...e to precision parts L utilisateur ne doit pas essayer de réparer cet équipement NE PAS tenter d ouvrir le boîtier ou de démonter ses pièces internes Seul un représentant du service PerkinElmer qui est conscient des risques d incendie et d électrocution devrait réparer cet équipement Cet équipement contient des parties à haute tension présentant des risques de blessure grave ou d électrocution au ...

Page 12: ...t obstructed If the ventilation is obstructed the system may overheat and catch fire Periodically clean your equipment s case using a damp cloth Do not use abrasives cleaning solvents or strong detergents as they may damage the finish or affect the reliability of the structural components Gardez l équipement exempt de poussières Nettoyez le cordon d alimentation régulièrement Les poussières qui s ...

Page 13: ... included in the Lambda 265 Spectrophotometer shipping kit Item Name Qty Main Instrument Lambda 265 Spectrophotometer 1 Accessories Power Cord US EU UK 1 1 1 Interface Cable USB 1 Fuse AC 250V T2 0AL 2 Wrench 3mm 1 Document Pack CD 1 UV Lab Software CD 1 Single Cell Holder 1 ...

Page 14: ...inElmer part numbers unless stated otherwise Notes cautions and warnings Three terms in the following standard formats are also used to highlight special circumstances and warnings NOTE A note indicates additional significant information that is provided with some procedures Note attention et avertissement Trois termes dans les formats standard suivants sont également utilisés pour mettre des circ...

Page 15: ...uadros éste se proporciona información sobre este tipo de circunstancias F Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves détériorations de l instrument ou d autre matériel Les détails sur ces circonstances figurent dans un encadré semblable à celui ci I Caution Attenzione Con il termine CAUTION ATTENZIONE vengono segnal...

Page 16: ...los recuadros como éste se proporciona información sobre este tipo de circunstancias F Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels à l utilisateur ou à d autres personnes Les détails sur ces circonstances sont données dans un encadré semblable à celui ci I Warning Pericolo Con il termine WARNING PERICOLO vengono seg...

Page 17: ...com PerkinElmer s catalog service offers a full selection of high quality supplies To place an order for supplies and many replacement parts request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 4000 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outside of the U S call your local PerkinElmer sale...

Page 18: ...18 Lambda 265 Users Guide ...

Page 19: ...Introduction 19 Safety Practices ...

Page 20: ...The information provided does not cover every safety procedure that should be practiced Ultimately maintenance of a safe laboratory environment is the responsibility of the analyst and the analyst s organization Please consult all manuals supplied with the Lambda 265 and accessories before you start working with the instrument Carefully read the safety information in this chapter and in the other ...

Page 21: ...ent les précautions énumérées ci dessous Il est conseillé d afficher une copie des précautions sur l étagère d instrument ou à proximité The following precautions must be observed when using the Lambda 265 Be sure that the voltage of the Lambda 265 corresponds to the voltage used in your laboratory Les précautions suivantes doivent être respectées lors de l utilisation du Lambda 265 Assurez vous q...

Page 22: ...ative non condensing Indoor only Altitude up to 2000m If possible avoid any adjustment maintenance and repair of the opened operating instrument If any adjustment maintenance and repair of the opened instrument is necessary this must be done by a skilled person who is aware of the hazard involved Whenever it is likely that the Lambda 265 is unsafe make it inoperative The Lambda 265 may be unsafe i...

Page 23: ...Même quand l interrupteur d alimentation est en position Off des tensions élevées peuvent encore être présentes Des condensateurs à l intérieur de l instrument peuvent être encore chargés même si l instrument a été débranché de toutes les sources de tension The instrument must be correctly connected to a suitable electrical supply The supply must have a correctly installed protective conductor ear...

Page 24: ...be done by a PerkinElmer Service engineer Whenever it is possible that the instrument is no longer electrically safe for use make the instrument inoperative and secure it against any unauthorized or unintentional operation The electrical safety of the instrument is likely to be impaired if for example the instrument shows visible damage has been subjected to prolonged storage under unfavorable con...

Page 25: ... is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Installation Category This product will operate safely in electrical power environments that are rated as Installation Category II in EN IEC 61010 1 Pollution Degree This prod...

Page 26: ... an instrument it means refer to the manual Lorsque cette étiquette est attachée à un instrument il est nécessaire de voir le manuel See the following figure for the location of warning labels on the Lambda 265 Warning labels on the back of the instrument Warning FUSE Avertissement FUSE ...

Page 27: ...the customer s environment Requirements for waste collection reuse recycling and recovery programs vary by regulatory authority at your location Contact your local responsible body e g your laboratory manager or authorized representative for information regarding applicable disposal regulations Contact PerkinElmer at the web site listed below for information specific to PerkinElmer products Web ad...

Page 28: ...Decontamination These documents are available on the PerkinElmer public website Procedure http las perkinelmer com Content technicalinfo dts_instrumentdeconprocedure pdf Certificate form http las perkinelmer com Content technicalinfo dts_perkinelmercertificationofdecontaminatio nform pdf If you do not have access to the internet and are located in the U S call toll free at 1 800 762 4000 or 1 203 ...

Page 29: ... que le cordon d alimentation est encore branché The fuse is located at the rear of the instrument 1 Turn off and unplug the instrument 2 Carefully open the compartment latch where the fuse is located 3 Disconnect the fuse 4 Replace with a new T2 0AL fuse One spare is contained in the power module 5 Close the compartment door 6 Plug in the instrument and turn on Location of latch on the fuse compa...

Page 30: ...30 Product Title Hardware Guide ...

Page 31: ...Lambda 265 Installation ...

Page 32: ...ting an electromagnetic field and any environment that may cause vibration Compte tenu des composants électroniques dans le Système Lambda 265 il doit être placé très loin de tous les autres instruments émettant des champs électromagnétiques et toutes les autres sources produisant des vibrations Power A grounded AC power supply must be used The Lambda 265 accepts 100 to 240 VAC at 50 Hz or 60 Hz W...

Page 33: ...pply AC 100 240V 50 60Hz 20W Operating Temperature 15 35 59 95 F Operating humidity Less than 80 relative non condensing Minimum Computer Requirements Requirement Description Hardware PC Intel Core 1 5GHz or faster processor At least 1 GB RAM 50 GB with 1 GB free HDD Color Monitor Display 1024 768 CD ROM Drive Mouse Keyboard USB port for the data acquisition Operating System Microsoft Windows 7 Ou...

Page 34: ...onnect the power cord to the plug the power plug must be grounded Figure 4 System in location WARNING The electric power could be dangerous to the body Before connecting the power cord verify that the main power switch is off Le courant électrique peut être dangereux Avant de brancher le cordon d alimentation vérifiez que l interrupteur du secteur soit en position Off 5 Setup the up the computer u...

Page 35: ...UV Lab Software Installation 35 UV Lab Software Installation ...

Page 36: ...ftware 1 Insert the UV Lab installation CD into the CD ROM drive InstallShield Wizard starts automatically 2 InstallShield Wizard does not start automatically select Computer CD Drive Double click setup exe 3 UV Lab InstallShield Wizard starts Select Next ...

Page 37: ...ation 37 4 Enter customer information if necessary and select Next 5 The software will be installed in C UV Lab as a destination folder To install the software to a different folder select Change and select the desired folder ...

Page 38: ...38 Lambda 265 Users Guide 6 Verify the current settings and select Install 7 The following window shows the status of the UV Lab installation ...

Page 39: ...UV Lab Software Installation 39 8 After the setup is completed successfully select Finish The UV Lab software icon will appear on the Desktop ...

Page 40: ...40 Lambda 265 Users Guide ...

Page 41: ...Interface Setup ...

Page 42: ...Guide 42 Installing the USB Drive 1 Turn on the computer And then insert the enclosed CD into the CD ROM drive 2 Connect the USB between the computer and instrument 3 Click Computer CD Drive Open 4 Click USB Drivers folder ...

Page 43: ...Interface Setup 43 5 Select LAMBDA 265 folder 6 Select Windows XP_ 7_8 Vista Server 2003 to 2008 win2000 R2 folder ...

Page 44: ...44 Lambda 265 Users Guide 44 7 Double Click USBCom CDM_20828 8 Select Extract ...

Page 45: ...e Setup 45 9 Select Next 10 The following dialog box will appear After installation is completed successfully select Finish 11 Change the USB serial Ports after finishing driver setup See the following chapter ...

Page 46: ...46 Lambda 265 Users Guide 46 Setting USB Serial Ports 1 Select Computer Properties 2 Select on Device Manager ...

Page 47: ...COM LPT to expand the listing These are the devices currently connected to the COM ports USB Serial Port COMx is visible when the driver installation is completed successfully 4 Double click USB Serial Port COMx of the Ports COM LPT section ...

Page 48: ...48 Lambda 265 Users Guide 48 5 Select on the Port Settings tab and select on the Advanced button 6 Change the parameter values as shown below 1024 1024 5 500 500 ...

Page 49: ...If the Lambda 265 fails to communicate with the PC change the COM Port Number as following procedure 10 Open Advanced Setting for COMx window following procedure on page 469 11 Select on the COM Port number list to expand it and change the COM port number another one which is not in use from COM 1 to COM 10 ...

Page 50: ...50 Lambda 265 Users Guide 50 12 Make sure that the changed COM Port Number is applied and select OK 13 Restart the computer after finishing driver setting ...

Page 51: ...Operating Procedure 51 Operating Procedure ...

Page 52: ...ter a Title Sample Name and choose the Experiment Type Select OK 4 Set method parameters Select OK 5 Prepare blank and sample solutions 6 Fill a 10 mm pathlength cell to 80 of its volume with the blank solution and place it into the cell holder Select or select Run Blank from the Measure menu ...

Page 53: ...move the cell from the cell holder 9 Fill a 10 mm pathlength cell to 80 of its volume with the sample solution and place it into the cell holder Click or select Run Sample in the Measure menu 10 The sample spectrum is displayed 11 Print and Save the spectral data ...

Page 54: ...54 Accessories ...

Page 55: ...ent parts and accessories can be ordered directly from PerkinElmer using the part numbers quoted in the guides provided with the instrument See our website http perkinelmer com PerkinElmer s catalog service offers a full selection of high quality supplies To place an order for supplies and many replacement parts request a free catalog or ask for information If you are located within the U S call t...

Page 56: ...in a standard 10 mm rectangular cuvette Optional stirring is available with a separately purchased stir controller This accessory accommodates a temperature by circulator Variable Pathlength Holder Use this accessory to hold rectangular cells with 5 10 20 40 50 mm pathlengths A moveable block secures cells in position ...

Page 57: ...se this accessory to test coatings and semi conductor wafers especially effective for measuring film thickness Peltier Controlled Single Cell With Peltier Controller Control the temperature of a single 10 mm pathlength rectangular cell between 5 and 100 This accessory includes a temperature controller Peltier accessory and all accessories necessary for its operation and connection to the instrumen...

Page 58: ...der Use this accessory to measure eight samples automatically This accessory accommodates a temperature by circulator This accessory is controlled by UV Lab software Autosampler Automate the measurement of up to 360 samples at a time with an autosampler accessory Two and four rack configurations are available This accessory system including the automated sipper is fully controlled by UV Lab softwa...

Page 59: ... accessory to measure micro volume sample such as DNA RNA and Protein Magnetic Stirrer Assembly Use this accessory to mix sample this accessory is controlled by Control Unit Control the speed of magnetic stirrer between 120 and 1200 rpm ...

Page 60: ...Notes cautions and warnings 14 text 14 D Decontamination 28 E Electrical Safety 23 Environmental Requirements 33 I Installation Lambda 265 34 SB drive 42 L Laboratory Preparation 32 O Operating Conditions 22 Operating Procedure 54 P Precautions 21 S Safety Summary 10 Safety Information 21 electrical safety 23 Shipping Kit 13 U USB drive installation 42 USB Serial Ports seetup 47 ...

Page 61: ...Accessories 61 ...

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