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PerkinElmer FlexDrop iQ, User Manual

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PerkinElmer FlexDrop iQ User Manual Download Page 18

 

Dispensing Runs 

18 

P/N CLS155330 Rev. A 

FlexDrop iQ User Manual 

PerkinElmer, Inc. 

Copy Wellgroups 

Whole wellgroups with already defined dispensing option, volume, and liquid 
can be copied by clicking the   

Copy Wellgroup

 button of the respective 

wellgroup. Select the liquid you want to use and the location for the new 

wellgroup (see Figure 12). 

 

Figure 12. Copy Wellgroup - Setup Screen 

 

 

Summary of Contents for FlexDrop iQ

Page 1: ...FlexDrop iQ Non Contact Dispenser User Manual Copyright 2021 PerkinElmer Inc P N CLS155330 Rev A Publication Date April 13 2021 ...

Page 2: ... disclaimer of express or implied warranties in certain transactions therefore this statement may not apply to you PerkinElmer reserves the right to revise this publication and to make changes from time to time in the content hereof without obligation of PerkinElmer to notify any person of such revision or changes Further PerkinElmer may make modifications to the product described in this manual a...

Page 3: ...gineers and in house Specialists who are dedicated to supporting your hardware software and application development needs Phone US Toll Free 800 762 4000 Worldwide 1 203 925 4602 Fax 1 203 944 4904 Email global techsupport perkinelmer com Our programs can include such useful services as Preventive maintenance Diagnostic servicing performed on site by PerkinElmer field service engineers or remotely...

Page 4: ...iption Description WARNING Caution Refer to the User s documentation ISO 7000 0434B AVERTISSEMENT Attention Se reporter à la documentation de l utilisateur NOTE A cautionary statement an operating tip or maintenance suggestion may result in instrument damage if not followed REMARQUE Énoncé indiquant une précaution à prendre un conseil de fonctionnement ou une suggestion d entretien son non respect...

Page 5: ...ernatif On power IEC 60417 5007 Marche alimentation Off power IEC 60417 5008 Arrêt alimentation CE compliance mark Marque de conformité CE WEEE symbol EN50419 2005 Do not dispose of as unsorted municipal waste See the PerkinElmer website www perkinelmer com for more information Consult Instructions for Use ISO 7000 1641 Consulter les Instructions d emploi ...

Page 6: ...col Overview 21 Starting the Dispense Protocol 22 Viewing the Results 23 Shutting Down the FlexDrop iQ 24 Print Log 25 Comma Separated Values CSV File 26 Header Section 26 Task Section 27 Advanced User Settings 28 About Liquids and the Liquid Library 28 Liquid Library 28 Liquid Class Library 29 Creating a New Liquid Class 30 Automated Calibration for New Liquid Classes 31 Manual Calibration of New...

Page 7: ...v A FlexDrop iQ User Manual PerkinElmer Inc Introduction Overview This FlexDrop iQ User Manual contains information about the safe and proper handling and operation of the FlexDrop iQ Non contact Dispenser and FlexDrop iQ consumables ...

Page 8: ...alize 3 On the tablet touch the FlexDrop iQ Studio icon to start the FlexDrip iQ Studio software You are prompted to Open a Saved Protocol or Create a New Protocol as shown in the figure below Figure 1 Starting Screen of the FlexDrop iQ Software 4 If the desired protocol has not been saved touch the Create New Protocol button and continue with Selecting the Protocol Settings on the next page If th...

Page 9: ...mes the source wells to make sure there are no air bubbles directly above the orifice o Specify the volumes for priming each source well o Specify the number of priming cycles for each source well Select the Error handling if no droplets are detected during priming Ask Abort or Continue Select the Optimization Level reduces dispensing time by reordering dispensing steps and using parallel dispensi...

Page 10: ...s enabled 2 Click Ok to save the settings and display the main window to choose the Wellgroup NOTE To edit the Target Plate properties click the Edit button in the top left corner of the Target Layout window NOTE If a barcode reader is available barcodes can be scanned Enable the barcode reader in the Advanced Device Settings see page 28 and click the Scan button to scan 3 Select the wells which s...

Page 11: ...Manual PerkinElmer Inc Figure 4 Target Layout Screen with Blue Highlighted Wellgroup NOTE To add additional target plates click the Add button in the top left corner of the Target Layout window The arrow buttons switch between different target plate layouts ...

Page 12: ...uid to the selected wells on the target plate Figure 5 Dispensing Option Mixing NOTE When performing dispensing runs by concentration the FlexDrop iQ software is able to calculate the required volume as long as all parameters total assay volume liquid stock concentration and desired concentration in target well are given Define the Total assay volume for the whole target plate by clicking the Edit...

Page 13: ...The following items can be edited Volume or concentration Set volume concentration start and end point for Logarithmic Auto and Linear distribution Set starting volume concentration for Custom distribution Add additional 0 step to dilution series Distribution Choose distribution type of the dilution series Linear logarithmic and individual dilution factors can be applied Add replicates Change dilu...

Page 14: ...ution series Figure 7 Dispensing Option Normalization Select one of the normalization options Highest fluid volume Equalize wells to the same total volume using the well with the highest volume level of the wellgroup Specific volume Equalize wells to a user specified volume Specific of assay volume Equalize well volume to the specified percentage of the total assay volume ...

Page 15: ...rray Specifying Array Size and Position The following items can be edited ID Select a pre defined array or create a new array Size X Y Specifies the array size spot number Pitch X Y Specifies the distance between spots Location Specifies the position of the array within the well Choose the wells to be dispensed and the desired dispensing option see Figure 9 ...

Page 16: ...ure 9 Defining Dispensing Options within an Array The selected array pattern will be applied to all chosen wells of target layout Figure 10 Final Array in Target Layout NOTE Mixing Dilution Normalization and Array will affect all wells of the selected area in the respective wellgroup ...

Page 17: ...in each corner of the area Change the size of an area by hovering on a blue square until the square turns red and then click and drag to the desired size Change the position of the whole wellgroup by clicking in the middle of the wellgroup and then click and drag to the desired position on the target plate Remove selected wells by dragging them to the bin icon see Figure 11 or pressing the Del key...

Page 18: ...llgroups Whole wellgroups with already defined dispensing option volume and liquid can be copied by clicking the Copy Wellgroup button of the respective wellgroup Select the liquid you want to use and the location for the new wellgroup see Figure 12 Figure 12 Copy Wellgroup Setup Screen ...

Page 19: ... edited Optional Enter a Plate Identifier Optional Add the Plate Barcode ID Select the source Plate Type Click Ok to proceed NOTE Edit the Source plate properties by clicking the Edit button in the Source Layout window To add additional source plates click the Add button on the top of the Source Layout window All liquids and the minimum required volumes for dispensing used in the protocol are list...

Page 20: ...either by manually dragging them to the desired position on the source plate or automatically by clicking Auto Assign All Assigned liquids move from the left to the right side of the screen into the Assigned liquids section Figure 15 Source Plate Setup Assigned Liquids After defining the source plate layout click Overview in the top bar to proceed ...

Page 21: ...ed to dispense the whole protocol and the total number of dispensing steps are shown Click Start Simulation to review all dispensing steps Adjusting the simulation speed enables speeding up or slowing down the simulation whereas adjusting the dispensing step allows you to observe specific steps of the protocol Figure 16 Overview Simulation Mode Screen Click Dispense in the top bar to proceed ...

Page 22: ...The FlexDrop iQ software prompts you to insert the source plate into the upper tray and the target plate into the lower tray of the device Figure 17 Dispense Screen Dispense Protocol 3 To pause the protocol at any time during the run click the Pause button 4 To stop the protocol at any time during the run click the Abort Dispensing button Source wells might have to be refilled with liquid and the ...

Page 23: ...ecified threshold All red wells received a volume lower than the requested volume and are below the specified threshold All used liquids are displayed on the left side of the screen with the corresponding position on the source plate The FlexDrop iQ software provides information on the performance of each source well Selecting any target well displays all liquids and volumes dispensed into the sel...

Page 24: ...the Source and Target drawers 2 Close the Source and Target plate drawers 3 In the FlexDrop iQ Studio software select Menu Exit to close the FlexDrop iQ Studio software 4 On the tablet select the Windows button then select Power Shut Down Wait until the tablet power turns off 5 Switch the instrument power switch on the back of the instrument to the Off O position ...

Page 25: ...sion of the device Software Build Version Software release date Source Plate ID Name of used source plate and source plate type Target Plate ID Name of used target plate and target plate type Liquid Name of liquid used for the respective dispensing step Source Source well position for the respective dispensing step Target Target well position for the respective dispensing step TargetFwd Side Targe...

Page 26: ...FlexDrop iQ Studio Protocols by default The CSV file used on the FlexDrop iQ device consists of a header and a task section Figure 20 CSV Format for the FlexDrop iQ Application Header Section The structure and content of the header section is Description of the CSV File Header Part 1 of 2 Cell number Content Meaning A1 Name of the protocol B1 FlexDrop iQ Studio software version C1 User of the prot...

Page 27: ...nter position of source well for respective dispensing step Target Well Enter position of target well for respective dispensing step Volume µL Enter volume in microliter for respective dispensing step Liquid Enter exact name of liquid for respective dispensing step NOTE The structure and content of the header section is crucial for proper import of the csv files into the FlexDrop iQ software The f...

Page 28: ...iquids are shown with their stock concentration and chosen liquid class Liquids can be deleted or added Assign favorite liquids by selecting the blue star in front of a liquid Favorite liquids display in the drop down list when choosing a liquid for any dispensing option while creating a protocol To choose a liquid that is not a favorite click Show All Liquids select the liquid name and click the ...

Page 29: ...ss Library contains the dispensing parameters for different liquid types like aqueous liquids DMSO glycerol solvents etc for the FlexDrop iQ Source Plate Add new liquid classes either by importing provided Liquid Class files libx or creating new liquid classes NOTE Liquid classes can only be deleted on the Advanced Settings tab For information about Advanced Settings see page 36 REMARQUE Les class...

Page 30: ...Overview 2 Type a name for the new liquid class and click Define 3 Choose one of the following options to define a new liquid class Copy from existing Dispensing parameters are copied from an existing liquid class which can be edited thereafter Measure Automated calibration of the parameters Detailed description in Automated Calibration for new liquid classes on page 31 Manually Enter parameters m...

Page 31: ...ss Manager guides you through the steps to measure and set up individual liquid classes Figure 25 Automated Liquid Class Determination Perform the following steps to measure a liquid class Step 1 Define the position of the source well for liquid class calibration Step 2 Select the target plate format and position for waste disposal Step 3 Select the liquid volume in the source well ...

Page 32: ...culates the liquid class from the generated data To show the resulting graph click the arrow next to the table The graph can be intersected with 0 0 by enabling the respective checkbox see Figure 26 Figure 26 Measured Values and Plotted Graph of Automated Calibration of Liquid Classes Select Save and Finish to save the liquid class in the Liquid Class Library For more information on how to adjust ...

Page 33: ...ge est fortement recommandée Plus le volume de remplissage de l étape 3 est élevé plus le résultat sera précis La mesure des triplicats est recommandée pour confirmer que les volumes de gouttelettes mesurés sont stables NOTE As soon as the liquid class is saved it cannot be edited anymore REMARQUE Dès que la classe liquide est enregistrée elle ne peut plus être modifiée ...

Page 34: ...he pressure pulse N Offset Specifies the intersection of the graph with the Y axis Default 0 nL Min Minimum dosing energy Specifies the lowest pressure to be applied for the respective liquid class Default 50 La Max Maximum dosing energy Specifies the highest pressure to be applied for the respective liquid class Default 300 La Select Save and Finish to save the liquid class in the Liquid Class Li...

Page 35: ...e format name Optional The plate manufacturer s name Length Width Height The outer dimension of the plate Number of Rows Columns Number of Rows or Columns on the plate Optional Cavity Volume The maximum filling volume of the plate well Distance to First Row Columns The distance from the plate edge to the center of the first well as shown in the Target Plate Editor Distance between Row Columns The ...

Page 36: ...ice Settings Screen Accessing the Advanced Settings Dispense Head Settings If the FlexDrop iQ dispenses incorrect volumes re calibration of the device might be required CAUTION Before re calibrating the FlexDrop iQ please contact your PerkinElmer Service Representative Do not change values in Advanced Settings without instruction from PerkinElmer personnel Misuse can cause damage to the device ATT...

Page 37: ...lider values of every single channel manually 2 Automated re calibration of the device by clicking Check Calibration see Figure 30 Figure 30 Advanced Settings Dispense Head Settings with Automated Re Calibration When using the automated re calibration the Dispense Head Calibration window guides you through the calibration see Figure 31 Figure 31 Dispense Head Calibration Protocol Screen ...

Page 38: ...n of the device ATTENTION Utilisez une pipette étalonnée uniquement pour le réétalonnage de l appareil The FlexDrop iQ counts the number of droplets dispensed and calculates the deviation to the expected number of droplets Clicking Update calibration moves the current FlexDrop iQ slider values by the offset of the deviation previously calculated see Figure 32 Figure 32 Dispense head calibration pr...

Page 39: ...d or modified click Reset Liquid Classes in the General Settings window Figure 33 Figure 33 Restoring Original Liquid Classes A message box displays the Liquid Classes that will be reset and prompts you to confirm that you want to reset the liquid classes listed Click OK to reset the liquid classes or Cancel to retain the existing changes Reset liquid classes does not delete new liquid classes ...

Page 40: ...PerkinElmer Inc 68 Elm Street Hopkinton Massachusetts 01748 U S A TEL 203 925 4602 FAX 203 944 4904 http www perkinelmer com ...

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