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PerkinElmer AAnalyst 300

Location: Friedland 202

PerkinElmerAA300.jpg: 

How It Works and What It Does

The AA-300 is used to determine the unknown concentration of an individual element by measuring the amount of light the unknown
concentration absorbs. Instrument readouts are calibrated to display concentration directly. The basic instrumentation requires a primary light
source, an atom source and a detector. The light source is a hollow cathode lamp (HCL) or electrode discharge lamp (EDL), which emits a
spectrum specific to the element from which it is made. The light beam passes through either an air-acetylene or nitrous oxide-acetylene flame,
which has a high temperature and specific flow rate. The atom source introduces the sample into the flame as an aerosol and the heat produces
free analyte atoms. Analyte atoms in the flame that correspond to the particular lamp being used absorb energy from the light source. A detector,
usually a photomultiplier tube, measures the light intensity exiting the flame. The electronics convert the amount of light absorbed to the actual
sample concentration.

 

The AA-300 is equipped with an automatic six-lamp turret and is programmed to perform sequential multielement analysis. The AA-300 is
equipped with lamps for the following elements: Ag, Al, As, Ba, Ca, Cd, Cr, Cu, Fe, K, Na, Mg, Ni, Pb and Zn The Iron(Fe) and Copper(Cu) lamps
are the most commonly used.

Directions for Use

Instructions for Use

by Golda Yelaun

Instruction for AA – Flame Analyst-300.

 

Check the location of exhaust vent (should be located at least 4 meters from the Flame).
Check the pressure of the AA Gas Purifier assembly. Make sure the output pressure is between 51 and 65 psig. ( on the wall , behind the
instrument).

Summary of Contents for AAnalyst 300

Page 1: ... as an aerosol and the heat produces free analyte atoms Analyte atoms in the flame that correspond to the particular lamp being used absorb energy from the light source A detector usually a photomultiplier tube measures the light intensity exiting the flame The electronics convert the amount of light absorbed to the actual sample concentration The AA 300 is equipped with an automatic six lamp turr...

Page 2: ...he burner gas supplies to the spectrometer Exit the software including the main application and the Windows software Switch off the Spectrometer Switch off the computer Empty the burner drain vessel Dispose the waste solutions according the Safety Regulations Instrument Calibration Curve correction Quantitative measurements in Atomic Absorption is based on Beer s Law which states that concentratio...

Page 3: ...eadings generated by the AA should be close together If the numbers jump around that indicates that the AA should be cleaned This might be needed after using a sample that hadn t been filtered well If the absorbance signal is low there may be a problem with the lamp Note If the AA is generating unexpected readings check that the trap flask under the machine is not too full If it is too full fumes ...

Page 4: ...Yelaun for more information Click here for departmental directory Main Users of Instrument The main users are General and Honors General Chemistry Chem 18 and 19 Occasionally other advanced chemistry courses use it too Web Resources Misc AA300 PerkinElmer info ...

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