Elizabethtown College Department of Chemistry
Last Updated by KK 1/09
15.
Close the Manual Analysis window. To view the calibration curve, select Calib. To view the
results of the sample analysis, select Results. To retrieve the last page of the printed output, select
New Page from the Print command list. Affix printed hard copies into your notebook.
16.
SHUTDOWN PROCEDURES
a.
After the final solution has been aspirated, allow at least three minutes of pure
deionized water to aspirate through the burner head to remove all reagents.
b.
Open the Flame Control window, and click on the Flame icon. The system will
automatically extinguish the flame safely.
c.
Turn off the burner gas supplies to the spectrometer.
d.
In the Flame Control window, click on the Bleed Gases button to depressurize the
gas lines.
e.
Wait at least 10 minutes after you have extinguished the flame, and then turn off the
fume ventilation system using the switch behind the instrument.
f.
Exit the software and Windows, and turn off the spectrometer, the computer, and the
printer.
g.
Empty the drain vessel, being certain to dispose of waste properly.
h.
Store samples and standards or dispose of them properly. They should not be left
near the instrument.
i.
Double check that you carried out each of steps 16a-h before exiting the lab.
FOR GRAPHITE FURNACE MEASUREMENTS:
9.
Turn on the Argon supply, and adjust the output to 50 psi.
10.
Assure that the PE Accessory Cooling System (found below the instrument) is on and that the
water level is in the appropriate range for use.
11.
Turn on the power to the Furnace using the switch on the left-hand side of the instrument.
12.
On the computer, select and launch the AA WinLab Analyst Software. The software will initialize
and establish communication with the spectrometer and the graphite furnace. When prompted,
select “Use the basic system procedure for…” and choose the setup designated by your instructor.
Alternatively, you may develop your own setup by consulting the appropriate section in the
instrument manual.
13.
If necessary,
a.
align the lamp according to the procedures outlined in the
AA Winlab Software
Guide for Atomic Absorption Spectroscopy
, Section 3-3.
b.
check the graphite tube in the furnace as described in the
HGA-800 Graphite
Furnace System including the AS-72 Autosampler Hardware Guide
, Section 2-19.
c.
condition the graphite tube as described in the
HGA-800 Graphite Furnace System
including the AS-72 Autosampler Hardware Guide
, Section 2-15.
d.
fill and prime the rinsing system as described in the
HGA-800 Graphite Furnace
System including the AS-72 Autosampler Hardware Guide
, Section 2-27.
e.
align the autosampler arm as described in the
HGA-800 Graphite Furnace System
including the AS-72 Autosampler Hardware Guide
, Section 2-28.
