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Carbon Dioxide Gas Sensor

Introduction

2

012-14330C

NOTE: If more distance is needed between the sensor and 
the interface, plug the sensor into the included Extension 
Cable, and then plug the cable into the interface. 

Insert the sensor probe into the gas sample. Allow 90 
seconds for the sensor to warm up with the probe tem-
perature between 20° and 30° C. 

Start the PASCO data collection software. 

Using the PASCO Data Collection Software

Set up a data display (such as a Digits or Graph display) 
in the software.

In PASCO Capstone, click one of the display templates 
in the workbook page, or double-click an icon in the 
“Displays” palette to open a data display. 

In the SPARKvue sensor parameter screen, touch ‘CO2 
Concentration’ to highlight it, and then touch ‘Show’ to 
open a Graph display. 

Click “Record” (in Capstone) or touch ‘Start’ in 
SPARKvue to begin recording data.

CAUTION:

The sensor was not designed for use in liquids, dusty or 
windy environments, or in direct sunlight. Do not place the 
sensor in liquid samples such as water or beverages as this 
will permanently damage the sensor. Using the sensor in 
dusty or windy environments or in direct sunlight may cause 
anomalous readings. The operating temperature range is 
between 20° and 30°C.

NOTE

: Do not try to remove the probe from the sensor. It is 

not removable.

STORAGE:

Always store the sensor in the plastic zip-lock bag to mini-
mize distorted sensor readings due to dust and dirt. 

Collecting CO

2

 Samples

Use the sampling bottle to collect air samples, such as an 
atmospheric sample or the carbon dioxide gas produced from 
germinating seeds.

To collect an atmospheric sample

Keep the bottle upright and the neck 
open (without the stopper). Wait long 
enough to allow atmospheric air to fill 
the bottle. Insert the probe with the 
stopper into the neck of the bottle. Be 
sure that the stopper fits snugly into the 
neck of the bottle.

To collect expired air or other 
gas samples

Collect the sample in a plastic bag and 
clamp the bag closed. To take a read-
ing, open the bag and immediately 
place it over the probe and stopper, holding the bag tightly 
around the stopper. 

Sensor Calibration

Because the sensor is 
factory calibrated, cali-
bration is not required 
for most activities. 
However, the sensor’s 
characteristics may 
change over time. To 
restore the sensor to its 
factory-calibrated 
state, perform the cali-
bration using a fresh air sample. 

(

NOTE

: Refer to the User’s Guide and On-Line Help for the 

data collection software.)

Collect a sample of fresh air (400 to 450 ppm CO

2

).

Insert the probe with stopper into the upright sampling 
bottle. Be sure that the stopper fits snugly into the neck 
of the bottle.

Connect the sensor to a PASPORT-compatible interface. 
Set up a digits displayand start recording data. Wait at 
least 90 seconds for the reading to stabilize.

Press the Calibrate button (CAL) for 3 seconds and then 
release it. The green light emitting diode (LED) will 
start blinking once per second to indicate that calibration 
has begun.

Wait approximately two minutes. When the green LED 
stops blinking, calibration is finished.

Check that the reading in the data acquisition software is 
400 ppm ±50 ppm (parts per million).

Suggested Activities

Cellular Respiration of Peas

Put 1/4 cup of dry pea seeds in the sampling bottle. 
Insert the probe and stopper into the neck of the bottle. 

Connect the sensor to a PASPORT-compatible interface 
and start the data collection software. 

Sensor

Interface

stopper

probe

LED

Calibration 

Button

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