Appendix
Rev. 007 Date 2010-01-13
CyFlow
®
space Instrument Operating Manual
25/26
Warranty
12 months on all parts except filters, mirrors, other quartz or glass parts,
disposables and cuvettes
2. CyFlow
®
space Optics
Laser / Output
Red Diode Laser:
25 mW or 40 mW at 638 nm
Green solid-state Laser:
30 mW to 100 mW at 532nm
Blue solid-state Laser:
20 mW or 200 mW at 488 nm
Violet Diode Laser
100 mW at 405 nm
Ultra Violet Diode Laser
16 mW at 375 nm
Laser specifications may vary. Other lasers on request.
Detectors
1 to 9 (FSC, SSC, FL1, FL2, FL3, FL4, FL5, FL6, FL7)
Filters
Standard setup and filters for FSC, SSC, FL1-FL7
Video Flow Monitor
Colour CCD camera for video flow monitor
Optical Coupling
Standard objective mount with high numerical aperture objective, high numerical
aperture immersion gel coupling, e.g. for detection of weak cytokines (option)
Excitation Optics
Elliptical 15 µm x 70 µm at 488 nm
Other beam geometries upon request
3. CyFlow
®
space Fluidics
Flow Cuvette
Synthetic quartz flow cuvette (350x 200 µm) for laminar sample transport with
sheath fluid for fluorescence, forward and side scatter light detection
Sample Delivery
Computer controlled precision syringe pump for contamination-free sample
transport.
Built-in air pressure for sheath fluid. Sheath fluid pressure is adjustable from 0-300
mbar (Computer controlled). Default setting: 200 mBar
Sampling Volume
Continuous up to 1500 µl
200 µl for precision absolute counting
Other counting volumes upon request
Free sampling volume with syringe counting method
Flow Rates
1) Sample volume speed adjustable continously between 0 and 50 µl/s
2) Sheath fluid pressure continuously adjustable
Fluidics Volume
2 x 2-litre reservoirs for sheath fluid and waste
BioSafety System
Avoids sample droplets and sample cross contamination (computer controlled)
4. CyFlow
®
space Electronics and Computer
Electronics
Parallel realtime signal processing for each of the optical channels with selectable
linear, 3- or 4-decade logarithmic amplification (6-decade log amplifiers), pulse
height, area and width analysis for doublet discrimination, 16 bit analog-to-digital
converters, trigger on any parameter or all parameters
Computer Desktop:
Processor ≥ Pentium 4 3.0 GHz, ≥ 512 MB RAM (or equivalent)
(specifications continiously updated
Harddisk ≥ 160 GB
according to improved performance
Floppy disk drive 1.44 MB and DVD CD-RW
of available hardware and software
19” TFT monitor
components)
Keyboard and mouse
Microsoft Windows XP® professional
Microsoft Office® 2007 Basic
