CyFlow
®
Cube Instrument Operating Manual
Rev-05_2014-01-31
19/40
Volumetric Counting with Electrodes
This measuring mode uses the START and STOP electrodes of the sample port to define a fixed sample
volume. In a standard sample port this “counting volume” is 200 µl. In a pre-counting phase the sample is
acquired normally as in
continuous acquisition mode
. Reaching the START electrode the data are
cleared and the volumetric counting phase starts. Reaching the STOP electrode the counting procedure will
be terminated and a system cleaning cycle will be initiated automatically.
Cells in Region
The
Events in Region
measure mode allows to define a number of particles within a specified region to
operate as STOP condition (select the respective
MaxCount
function in the
REGIONS
register).
Schematic overview of the measure modes with a flash indicates clearing of the data. User selectable
criteria in green, software-defined values in red
Working principle of the Absolute Volumetric Counting with Electrodes
