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CyFlow

®

Cube 6

Instrument Operating Manual

CyFlow

®

Cube 6

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Summary of Contents for CyFlow Cube 6

Page 1: ...CyFlow Cube 6 Instrument Operating Manual CyFlow Cube 6...

Page 2: ...ec recommended IVD reagents The PartecCyFlow Cube Flow Cytometer complies with the European IVD Directive 98 79 EC and is therefore CE marked IVD Contact Information Partec GmbH Otto Hahn Stra 3 32 D...

Page 3: ...Histogram and dotplot in immunology 9 INSTRUMENT STARTING PROCEDURE 10 Sheath fluid level control and or refill 10 MAIN CYVIEW LOGIN WINDOW 11 USER LEVELS 12 CYVIEW MAIN PAGE AFTER SUCCESSFUL LOG IN 1...

Page 4: ...ties 26 Region name 26 Home Plot name 26 Color RGB 26 Max count 26 Sorter region 26 Moving of regions within a histogram 26 Create a vertical or horizontal histogram splitter 27 Create a quadrant asse...

Page 5: ...new version please let us know by sending an email or a note to Partec GmbH The present manual is valid as of CyView software version 1 5 For more details about the reagent kits suitable for use with...

Page 6: ...lly any flow cytometric application The applications cover Routine multi color immuno phenotyping Blood Cell Analysis HIV monitoring e g CD4 cell count Leukocyte Counting Rare Event Analysis Microorga...

Page 7: ...y axis a peak All cells having about equal characteristics among the analysed cell property e g content of a specific constituent like DNA form a peak In the case of a typical DNA histogram one peak r...

Page 8: ...ve size FSC on a logarithmic scale in X and the number of events on a linear scale in Y Two peaks are visible Example of a dotplot A 2D dotplot presents correlated data over 2 dimensions In the image...

Page 9: ...he cells presented in the previous dot plot stained with antibodies anti CD45 conjugated to PhycoErytrin PE Dy647 The X axis displays the fluorescence in a 4 dec logarithmic scale and the Y axis displ...

Page 10: ...ast once a week or before any daily use When filling up the sheath fluid bottle make sure no air bubbles are trapped in the yellow filter unit inside the bottle Make sure WASTE bottle is empty and the...

Page 11: ...loading of the setting files Searching Device display of the correct recognition of the connection between the computer and the embedded electronics Please verify that all operations are confirmed by...

Page 12: ...pe in Name and Password followed by Build new account Main user should be deactivated As User the own password can be changed by selecting the Name and placing a new Password followed by Change my pas...

Page 13: ...ace to acquire save re load and analyse your data Instrument real time display of workload L on board memory status M analysis volume V and analysis duration D Console PC control board link File opera...

Page 14: ...for dotplots A specific plot is selected with the arrow keys Define a Comment characterizing the plot e g FSC Select X axis channel and Y axis channel in dot plots or X axis channel only for histogra...

Page 15: ...mReg1 x DenomReg2 NumRegion1 2 defines numerators DenomRegion1 2 defines denominators NumOperator defines operator between 2 numerators x or DenomOperator defines operator between 2 denominators x or...

Page 16: ...switch between regions Confirm all modifications by pressing Accept GENERAL properties of the instrument The active Configuration File The instruments Serial Number Total Operating Time of the instru...

Page 17: ...e a volume can be pre selected Volumetric counting VC with electrodes START and STOP electrodes of the sample port were used to define a fixed sample volume of 200 l Cells in region a particle number...

Page 18: ...e stop electrode is reached even if the selected end criteria is not yet realized Measure modes The following measure modes can be selected prior to start of an analysis Analyze all Volumetric countin...

Page 19: ...d and the volumetric counting phase starts Reaching the STOP electrode the counting procedure will be terminated and a system cleaning cycle will be initiated automatically Cells in Region The Events...

Page 20: ...formed Starting the instrument for the first time Daily initialization After the Sheath fluid bottle was filled As Trouble Shooting procedure no bad signals blocking etc To start the priming initializ...

Page 21: ...move the Sample tube press Continue and wait until the system has finished Connect the Sample tube again press Continue and wait until the system has finished At the end press Continue The instrument...

Page 22: ...he following Select the CLEAN batch by clicking on the CLEAN batch symbol After pressing Start the system will start the priming process automatically and guide through the program Connect a sample tu...

Page 23: ...h cleaning solution and clean the instrument by clicking on the Clean symbol The instrument will perform one cleaning cycle Repeat the procedure with a sample tube filled with sheath fluid Please noti...

Page 24: ...ger 2 in SSC and FSC SSC The GAIN G and THRESHOLD T sliders must be adjusted to obtain the optimal gain maximum signal and minimum background Typically the Forward Scatter FSC will be the first gain t...

Page 25: ...he erosion level will set a threshold on the data displayed not the acquired data Some of the low frequency points will not be displayed allowing a better visual discrimination of the higher frequency...

Page 26: ...sical plot where the region is located Color RGB Allows to choose the color of the region This color will be reported to each plot selected as color gating only Max count This option allows fixing the...

Page 27: ...reate asymmetric quadrants please approach individual points at the border of the dotplot or at the intersection point of the quadrants with the left mouse button and keep the left mouse button presse...

Page 28: ...nly All Region Colors Compensation Press Compensation button to open the compensation Compensation Select a parameter combination to compensate Use the slide bar to set the compensation Clear Save and...

Page 29: ...gs will be load Perform PRIME process Fill a sample tube with 1 2 ml of your sample Check the tube for eventual imperfection or contaminants cracks aggregates hair and replace or remove resp if necess...

Page 30: ...the instruments set up or Space at the keyboard By default the continuous measure mode is pre selected In this case the measurement will only stop automatically when the sample is consumed An earlier...

Page 31: ...steps click and hold left mouse Region Shift Moves a region to the original position click and hold left mouse Shift Ctrl Moves the region by addition of an interval to the original position in very...

Page 32: ...nternational as well as national regulatory standards such as PPE personal protection equipment must be met for all users Therefore the system is marked with the following biohazardlabel Warning The W...

Page 33: ...and clean with warm detergent solution and a brush Clean sheath reservoir with distilled water and a clean brush and flush with clean distilled water several times Remember a clean sheath fluid reserv...

Page 34: ...nsport or storage please take care that the system will be stored under the following conditions Temperature 5 50 C Humidity 20 85 relative non condensing Room Clean environment no direct sun light Di...

Page 35: ...ted if the housing of the device is damaged and the protection cover for the laser beam is removed Therefore the system is marked with the following laser safety labels Warning It is prohibited to ope...

Page 36: ...in UV LED DAPI FL4 red fluorescence fluorescence origin 638 nm laser APC If required by a specific application the optical standard setup can be optimized by exchanging preassembled removable mirror f...

Page 37: ...counting and mechanical fluid volume measurement Absolute Counting No need for reference sample or beads Instrument Check Partec Count Check Beads Order No 05 4010 Partec Calibration Beads 1 m Order...

Page 38: ...r sheath water and sample volumes no fluid droplets or aerosols generated nor released from the instrument Flow Cuvette Synthetic quartz flow cuvette 350x 200 m for laminar sample transport with sheat...

Page 39: ...CyFlow Cube Instrument Operating Manual Rev 05_2014 01 31 39 40 Notes...

Page 40: ...Excellence in Flow Cytometry and Cell Analysis...

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