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XE-70 AFM Order of Operations 

 

1)

 

Turn on system, mount tip if needed 

2)

 

Focus microscope on cantilever 

3)

 

Position laser spot on end of cantilever 

4)

 

Turn on XEP software 

5)

 

Position laser reflection on center of photodetector (A+B ~3V, A-B and C-D ~0.3V) 

6)

 

Select appropriate mode, scanner, and frequency 

7)

 

Approach surface 

i.

 

Get optically close (several mm) 

ii.

 

Focus microscope ~1mm from surface, move Z-stage til cantilever in focus 

iii.

 

Repeat at ~100um from surface 

iv.

 

Click ‘Approach’ 

8)

 

Adjust scan parameters (Z servo gain, scan rate, set point, drive) 

9)

 

Collect images 

10)

 

Send to XEI to process/save 

11)

 

Follow shutdown procedure 

Summary of Contents for XE-70

Page 1: ...is is relayed to a motor controlling the tip which adjusts the tip s vertical position until the laser is aimed in the center of the quadrant photodetector once again Effectively this keeps the tip at...

Page 2: ...and place it in the good tips box Ensure new tip is rested in place and that bearings fit into tip slots 4 Replace head and push flaps away from you until slightly tight This secures the head positio...

Page 3: ...on t see the laser reflected off the cantilever and the laser is on use the Mirror 1 knobs to move the laser along the vertical axis until you see it reflected on the edge of the probe then move horiz...

Page 4: ...eeded until the sum is at the appropriate value If after several tries you still can t get the sum high enough remove the head and check mirror 2 s position visually it should be roughly parallel to t...

Page 5: ...o 1 00 Cantilever General 4 Click OK 5 Turn Head back on in software Frequency Sweep Window will open 6 The software will automatically detect the cantilever s resonance peak and select a driving freq...

Page 6: ...he tip into the sample which could damage it 10 When sample is in focus image of tip should be slightly blurry 11 Check laser position to ensure A B and C D are still less than 0 3 V 12 Shut acoustic...

Page 7: ...will change the distance the tip hovers above the sample surface when scanning and can improve resolution of features surface tracking This can be done manually by changing the number in the Set Poin...

Page 8: ...h XEI You can send images directly to the XEI data analysis software from the Buffer in XEP by right clicking on them Or you can open them from the spmdata or User Files folders Flattening your sample...

Page 9: ...f laser switch unplug head and remove head 6a If the tip you used was good leave it on the AFM Otherwise remove head and place tip in the Bad Probes box 7 Remove sample from stage and replace head plu...

Page 10: ...f photodetector A B 3V A B and C D 0 3V 6 Select appropriate mode scanner and frequency 7 Approach surface i Get optically close several mm ii Focus microscope 1mm from surface move Z stage til cantil...

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