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Instruction Manual 

PA201, PA202, & PA203

The MISCO Palm  Abbe refractometers are advanced 

fourth-generation handheld digital refractometers that put 

laboratory precision in the palm of your hand.  They are 

designed for the rapid and accurate determination of fluid 

concentrations. These instruments will automatically compen-

sate for temperature with computer precision, and when used 

and cared for properly, will provide years of trouble-free service. 

This manual will help you maximize the usefulness of your 

instrument and MUST be read completely before use. If you 

have any questions, please call: 

MISCO technical support at 440-349-1500.

The PA201 is equipped to take readings on the Brix scale and 

utilizes sucrose as the temperature compensation basis.

The PA202 is equipped to take readings on the Brix and Refractive 

Index (nD) scales. Both scales use sucrose as the temperature 

compensation basis. The PA202x may have up to two custom scales 

and may have special temperature compensation.

The PA203 may have from one to five different scales, each scale 

having an individual unit of measure and temperature compensation 

basis. 

To the extent that your particular instrument is equipped with scales 

other than those described above, the scale specifications will be 

documented on a specifications card accompanying the 

instrument.

INTRODUCTION

Specifications

The Span

 Point represents

 a calibration

 point 

towar

d the

 upper end

 of the

 Palm

 Ab

be 

range. Although it is important to Zero Set 

the Palm Abbe dail

y, you may at some time 

want to

 set the

 span adjustment

 as well.

 The

 

span should be set if you notice a general 

degradation of

 the instrument’

s accuracy

 and 

precision and/or

 on a

 periodic basis

 

determined by

 your

 particular

 history

 with the

 

instrument.

 

Factors af

fecting your

 decision to

 set the

 span 

adjustment include

 how often

 the instrument

 is used,

 how roughly

 it is

 handled, 

how often

 it is

 exposed to

 large temperature

 changes, and

 the level

 of 

precision you require for your measurements. 

To set the span, begin with a Zero-Set, then follow the procedures 

for zero 

setting on the opposite page but go to the 

SET SPAN ? / 

[go] to set

 

menu

 and

 substit

ute th

e ca

librat

ion f

luid fo

r w

ater

 in th

e pr

oce

dure.

 

The calibration fluid temperature for span setting must be 

between 15

 to 25°C

 (59 to

 77°F). If

 the span

 set is

 

successful, the

 instrument w

ill read

 “Ready

,” otherw

ise 

an error will be displayed. Follow up with a Zero-Set.

It is

 recommended that

 you use

 only a

 NIST 

traceable calibration 

fluid specific 

to the Palm 

Abbe. This fluid is available 

from MISCO by 

calling 440-349-1500

 or is

 available for

 

purchase online

 from the

 MISCO website,

 

at www.misco.com

 (p/n CALFLU202-3).

The Palm Abbe Refractometer MUST

 be Zero Set before initial use and 

periodically thereafte

r.  

It is recommended that a Zero Set be performed at 

least once 

a day as well as 

prior to performing tests 

requiring the highest 

precision, or when moving between environments 

with extreme changes in 

ambient temperature.

  

A clean container of water is all that is needed to automatically 

zero the 

instrument.  Although tap water 

may be used, distilled or deionized water is 

recommended.  

The water temperature for zero setting should ideally be 

between 10°C to 30°C (50° to 86°F).  

It is important to independently verify 

that you 

are indeed calibrating 

the instrument with water and 

not just a clear 

liquid which you think is water.  Never use an instrument unless you have 

personal knowledge of its calibration.

Calibra

tion - Zero Set

1.

Inspect the measuring surface to make sure it 

is clean and dry. 

Place a few 

drops of distilled 

water on measuring surface.

2.

Close the

 sample cover

 and allow

 some time

 

for the temperature to equalize. The sample 

cover MUS

T be 

closed to calibrate 

or take 

readings.

4.

Press and

 release  <MENU>

 until the

 

display reads:

Set Ze

ro?

[GO] 

To Set

5.

Press and

 release <GO>

 to automatically

 

zero

 the

 inst

rum

ent. R

emem

ber to

 clean

 an

dry the measuring surface after calibration.

3.

Press and

 release <G

O> to

 turn the

 

instrument on.

If the

 calibration

 was successful,

 the

 instrument

 will

 display

 “READY

,” 

otherwise there will be an error message displayed.

Calibra

tion - Span Set

Call MISCO for 

NIST Traceable

Certified Calibration

440.349.1500

Made in USA

Rev. 150201

PA201

PA202

PA203

Unit of Measure:

Brix

Brix

Refractive Index (nD)  Up to 5 Different Scales

Range:

0.0 to 56.0

0.0 to 85.0 Brix

1.3330 to 1.5040 nD 

Equivalent To

1.3330 to 1.5040 nD 

Resolution:

0.1 Brix

0.1 Brix

0.0001 nD 

Equivalent To

0.0001 nD 

Precision:

+/- 0.1 Brix

+/- 0.1 Brix

+/- 0.0001 nD 

Equivalent To

+/- 0.0001 nD 

Well Material:
Prism Material:

Auto Temp. Comp. 
Range:

Auto Temp. Comp. 
Basis:

Sucrose

Varies

Varies

Min. Sample Volume:
Measuring Time:
Power Supply:
Battery Life:

~3,500+ Readings

Dimensions:
Net Weight:

250 grams (8.8 Oz.)

Languages

5 Languages - English, French, Spanish, German, & Russian

Display:
Color:

Blue

Blue

Black

Stainless Steel

Sapphire

2 Lines x 12 Characters

145 x 75 x 37 mm (5.7 x 2.95 x 1.46 in.)

2 X AAA Batteries

0.3 ml

< 5 Seconds

0 to 50° C (32 to 122° F) Typical

®

Palm Abbe

Digital Refractometer

Menu Options

Various

 options are

 accessible through

 the <MENU

> button.

 With the

 

instrument “ON”, press and release <MENU> to step through the options.

SCALE #1

[GO] to Set

This option sets the default unit of measure, or scale, 

the instrument will read. The actual scale name will 

replace the words “SCALE #1” on the display. Some 

instruments are programmed with more than one 

scale. Pressing 

<MENU> will step through 

all available 

scales. Press <GO> to set a scale as the default

.  

SET ZER

O?

[GO] 

to Set

The Zero Set option will 

zero or calibrate 

the 

instrument to water. See “Calibration - Zero Set.”

 

set sp

an?

[GO] 

to Set

The Span Set option will calibrate 

the upper 

range of 

the instrument to a special 

calibration solution. 

See 

“Calibration - Span Set.” 

LCD-Li

ght?

[GO] YES

This option allows you to turn the LCD display 

backlight on or off. The options are “Yes” and “NO.”

Langu

age?

[GO] to Set

This option allows you to set the display to a particular 

language; English, Spanish, German, French, or 

Russian. Just follow the prompts to your language of 

choice and press <GO> to set. 

THE SAFETY INSTRUCTIONS AND WARNINGS PRESENTED 

BELOW AND ELSEWHERE IN THIS MANUAL ARE MINIMUM 

REQUIREMENTS FOR THE SAFE AND RELIABLE OPERATION 

OF THIS EQUIPMENT. THESE PRECAUTIONS MUST BE 

OBSERVED DURING ALL PHASES OF USE OF THIS EQUIPMENT. 

FAILURE TO COMPLY WITH THESE PRECAUTIONS OR WITH 

SPECIFIC WARNINGS GIVEN ELSEWHERE IN THIS MANUAL 

COULD RESULT IN DEATH OR SERIOUS INJURY.
READ THIS MANUAL BEFORE USING THIS EQUIPMENT. You 

must read and follow this Manual before operating this equipment. 

If you have any questions, contact MISCO technical support before 

use.
KEEP MANUAL AVAILABLE. Keep this Manual available for 

reference in a safe and accessible location. 
FLUIDS TESTED MAY BE HAZARDOUS OR TOXIC. You must read 

and follow all safety instructions and handling procedures in the 

Material Safety Data Sheet (MSDS) and/or Safety Data Sheet (SDS) 

for each fluid you choose to test. Pay careful attention to the informa-

tion concerning Hazards, and to the instructions and warnings for 

Handling and Storage, and Exposure Controls/Personal Protection. 

If testing bodily fluids, take precautions to prevent exposure. In all 

cases, properly discard any residual sample after testing.

WARNING

6275 Cochran Road

Solon, OH  44139-3316

TEL 440.349.1500

www.misco.com

Protected by US Patent D510880S

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