16
3.4 IMPORTANT SAMPLE TUBE INFORMATION
Dry, fresh or frozen samples can be homogenized with or without buffer using the Bead Ruptor 24
and Bead Ruptor sample tubes.
The intratube forces generated by the Bead Ruptor 24 during the homogenization process are up to 400 x g.
Intratube forces are dependent on the speed setting and the bead media present inside the tube. Omni pro-
vides unique tubes that are designed to withstand such high forces. It is not recommended to use tubes pro-
vided by other suppliers as these may not withstand to forces generated by the Bead Ruptor 24 and could
result in tube failure and loss of sample. Please reference the table below for Omni tube speed thresholds.
In addition to the intratube forces generated by the Bead Ruptor 24, sample heating can occur when process-
ing for multiple cycles or extended time periods. Excessive sample heating can result in tube leakage or failure.
Because the Bead Ruptor has the ability to run at up to 8 m/s, tube leakage and breakage could occur due
to heat and pressure build-up inside the tube.
In the event of tube leakage or tube breakage, Omni recommends the following solutions:
• Use the tubes as single-use or disposable
• Add more buffer to decrease the force applied by the beads to the tube walls
• Open the Bead Ruptor’s lid for 5 to 10 minutes in order to cool down the tubes and tube carriage between cycles.
• Place the sample tubes in an ice bucket for few minutes in between cycles
prior to run additional cycles.
• Ensure that the fingerplate is tightened securely and the lock is engaged.
• Ensure tube caps are tightened completely
CAUTION:
DO NOT
freeze Bead Ruptor Tubes in liquid nitrogen.
SECTION 3 — BEAD RUPTOR SAMPLE TUBES
3.2 ORDERING INFORMATION
50 mL PRE-FILLED BEAD MILL TUBES
TUBE SIZE
BEAD SIZE
DESCRIPTION
QUANTITY
ORDER NUMBER
50 mL
2.8 mm
Hard Tissue Homogenizing Mix
50
19-6508
BEAD MEDIA
Ceramic
Bulk 50 mL Screw Cap Sample Tubes (No Beads)
Empty sample tubes with caps. Beads are not included.
TUBE SIZE
DESCRIPTION
QUANTITY
ORDER NUMBER
50 mL
Polypropylene conical screw cap
tubes with caps
100
19-6650
50 mL Bead Tubes are not certified RNase/DNase Free, Maximum recommended speed is 5.0 m/s.
CAUTION:
2ml non reinforced tubes are not compatible with beads >2mm in diameter.
TUBE SIZE
SPEED
0.5ml
8 m/s
1.5ml
5 m/s
TUBE SIZE
SPEED
7ml
6.0 m/s
15ml
7.0 m/s
TUBE SIZE
SPEED
2ml non reinforced
8 m/s
2ml reinforced
8 m/s
TUBE SIZE
SPEED
30ml
5.0 m/s
50ml
5.0 m/s
SECTION 3 — BEAD RUPTOR SAMPLE TUBES
