Plant DNA Purification Kit
Problem
Cause
Suggestion
Clogged Column
Carry-over of debris.
Following extraction with
chloro:isoamyl alcohol, make
sure no particulate material is
transferred.
Sample too viscous.
Do not exceed suggested
amount of starting material.
Alternatively, increase
amounts of Buffers CTB and
BB and use two or more
columns per sample.
Low DNA yield
Incomplete disruption of
starting material.
For both dry and fresh
samples, obtain a fine
homogeneous powder before
adding CTB Buffer or use a
Bead Ruptor if possible.
Poor lysis of sample
Decrease amount of starting
material or increase amount
of CTB Buffer, chloro:isoamyl
alcohol, and BB Buffer.
DNA remains bound to
column
Increase elution total volume
to 200 μL and incubate
at 65oC for 5 min before
centrifugation.
DNA washed off
Dilute DW Buffer by adding
appropriate volume of absolute
ethanol prior to use.
Problems in
downstream
applications
Salt carry-over
DW Buffer must be at room
temperature.
Ethanol carry-over
Following the second wash
spin, ensure that the column is
dried by centrifuging 2 min at
maximum speed.
TROUBLE SHOOTING GUIDE
Please use this guide to troubleshoot any problems that may arise.
For further assistance, please contact the technical support staff, toll free, at
1-800-776-4431.
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