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PLANT DNA PURIFICATION KIT

User Manual

26-022 ............5 preps

26-023 ............50 preps

26-023B .........50 preps

P/N 03-293-10 REV. B

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Summary of Contents for 26-022

Page 1: ...PLANT DNA PURIFICATION KIT User Manual 26 022 5 preps 26 023 50 preps 26 023B 50 preps P N 03 293 10 REV B...

Page 2: ...1 Introduction Pg 1 2 Overview Pg 1 3 Kit Contents Pg 2 4 Storage and Stability Pg 3 5 Illustrated Protocols Pg 3 6 Before Starting Pg 4 7 Preparing Reagents Pg 4 8 Plant DNA Kit Directions Pg 5 10 T...

Page 3: ...RVIEW If using the Omni Plant DNA Purification Kit for the first time please read this booklet to become familiar with the procedures This procedure relies on the well established properties of the ca...

Page 4: ...L CTB Buffer 5 mL 40 mL 40 mL Antifoam 1 mL 1 mL 2 mL Bead Kit 2 8 mm ceramic 5 50 User Manual STORAGE AND STABILITY All of the Omni Plant DNA Purification Kit components are guaranteed for at least 1...

Page 5: ...ion Kit 3 ILLUSTRATED PROTOCOLS Lyse and homogenize sample Remove polysaccherides and polyphenols Adjust the binding conditions Transfer lysate into a DNA mini column to bind DNA Wash column x 2 Dry t...

Page 6: ...ree 1 5 mL microcentrifuge tubes Waterbath or incubator capable of 65 C Bead Mill Homogenizer or vortexer mercaptoethanol ME Chloroform and isoamyl alcohol 24 1 Optional RNase stock solution 20 mg mL...

Page 7: ...00 L CTB Buffer Vortex vigorously to mix Make sure to disperse all clumps NOTE Ensure ME is added to CTB Buffer 20 L ME 1 mL CTB Optional Add 5 L RNase Let sit at room temperature for 5 minutes Procee...

Page 8: ...e 15 Centrifuge at 10 000 x g for 1 minute 16 Discard the filtrate and reuse the Collection Tube 17 Repeat Steps 14 16 for a second DW Buffer wash step 18 Centrifuge the empty Omni DNA Mini Column at...

Page 9: ...lysis of sample Decrease amount of starting material or increase amount of CTB Buffer chloro isoamyl alcohol and BB Buffer DNA remains bound to column Increase elution total volume to 200 L and incuba...

Page 10: ...Plant DNA Purification Kit NOTES...

Page 11: ...Plant DNA Purification Kit NOTES...

Page 12: ...935 Cobb Place Blvd Kennesaw GA 30144 Phone 800 776 4431 Fax 770 421 0206 www omni inc com...

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