Omega Mag-Bind FFPE RNA 96, Product Manual

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M2551 Mag-Bind® FFPE RNA 96 Kit Protocol
Mag-Bind® FFPE RNA 96 Kit Protocol - 96-well plates
Materials and Equipment to be Supplied by User:
• Nuclease-free 1.2 mL round-well plates
• Nuclease-free microplates
• Centrifuge with swing-bucket rotor capable of 4,000 x g
• RNase-free filter pipette tips
• Magnetic separation device for 96-well plates (MSD-01B or MSD-01)
• Water bath or heat block capable of 55°C
• Water bath or heat block capable of 80°C
• Water bath or heat block capable of 37°C
• 100% ethanol
• Sealing film
Before Starting:
•
Prepare RNA Wash Buffer II and GFC Buffer according to the Preparing Reagents
section on Page 4
• Set water bath or heat block to 55°C
• Set water bath or heat block to 80°C
• Set water bath or heat block to 37°C
1. Add 250 μL RML Buffer into each well of a 1.2 mL round-well plate.
2. Cut 2-5 paraffin sample sections between 5-10 μm to be placed in each well of the 96
plate. Note: Do not use the first 2-3 sections.
3. Immediately place 2-5 sections into each well of the round-well plate.
4. Centrifuge at 4,000 x g at room temperature for 2 minutes.
5. Incubate at 80°C for 15 minutes to melt the paraffin. Mix the sample a few times by
gently shaking the tube. Make sure that the tissue sections stay submerged in the
solution.
Note:
Seal the plate with sealing film to prevent evaporation during incubation.