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3)
Raise the condenser till the top lens is close to the opening of stage. Place a drop of
immersion oil on the top of the lens of the condenser.
4)
Place the slide on the stage. Raise the condenser and let the oil drop
contact the bottom of the slide. If air bubbles exist in the oil, clean the
oil from the condenser lens and bottom of slide with a camera
cleaning paper and repeat the procedures.
5)
Follow the procedures in this manual to bring the sample on slide in
focus and observe.
6)
When using the 100X oil darkfield objective, you will need to drop the
immersion oil and adjust the iris ring (
Fig. 16
) to get proper
brightness and contrast of view field.
Note
：
The condenser won
’t work well if no oil drop applied on the condenser.
8
Phase Contrast Kit Installation and Operation
Instructions
8.1 Phase contrast kits of A1PHB1 or A1PHB3:
1) Mounting the phase contrast objectives
a. Take off all the objectives from the nosepiece.
b. Install the phase contrast objectives onto the nosepiece following the steps in 2.3.
2) Mounting the phase contrast condenser/annular ring disk
a. Rotate the nosepiece and set the 4x objective in position.
b. Turn the coarse focus knob to raise the mechanical stage to the highest position
without contacting the 4X objective.
c. Turn the condenser control knob to raise the condenser to the highest position.
d. Loosen the light collector and take it off.
e. Loosen the condenser lock thumb screw, pull down the condenser and take it off.
f. Insert the condenser/annular ring disk into the condenser holder, and tighten the
thumb screw.
g. Insert the light collector back onto the
base and turn until it’s tightened.
h. Re-adjust the condenser height to its normal position.
i. Re-adjust the mechanical stage height to its
normal position.
Note:
When raising the mechanical stage, do not
make contact with the objective.
3) Turn the desired objective into light path.
4) Turn the annular ring disk to put the corresponding
ring into light path, i.e. if you are using the 40x
phase contrast objective, you should turn the disk
at 40 as shown in
Fig. 17
.
Fig.16
Iris
Ring
20
Fig.17
