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- 16 - 

3) 

Raise the condenser till the top lens is close to the opening of stage. Place a drop of 
immersion oil on the top of the lens of the condenser.

 

4) 

Place the slide on the stage. Raise the condenser and let the oil drop 
contact the bottom of the slide. If air bubbles exist in the oil, clean the 
oil from the condenser lens and bottom of slide with a camera 
cleaning paper and repeat the procedures.

 

 

5) 

Follow the procedures in this manual to bring the sample on slide in 
focus and observe.

 

6) 

When using the 100X oil darkfield objective, you will need to drop the 
immersion oil and adjust the iris ring (

Fig. 16

) to get proper 

brightness and contrast of view field.

 

 

Note

 

 

The condenser won

’t work well if no oil drop applied on the condenser.

 

 
 
 

8

 

Phase Contrast Kit Installation and Operation   
Instructions 

8.1  Phase contrast kits of A1PHB1 or A1PHB3: 

1)  Mounting the phase contrast objectives 

a. Take off all the objectives from the nosepiece. 
b. Install the phase contrast objectives onto the nosepiece following the steps in 2.3. 

2)  Mounting the phase contrast condenser/annular ring disk 

a. Rotate the nosepiece and set the 4x objective in position.

 

b. Turn the coarse focus knob to raise the mechanical stage to the highest position 

without contacting the 4X objective.

 

c.  Turn the condenser control knob to raise the condenser to the highest position.

 

d. Loosen the light collector and take it off.

 

e. Loosen the condenser lock thumb screw, pull down the condenser and take it off.

 

f.  Insert the condenser/annular ring disk into the condenser holder, and tighten the 

thumb screw.

 

g. Insert the light collector back onto the 

base and turn until it’s tightened.

 

h. Re-adjust the condenser height to its normal position.

 

i.  Re-adjust the mechanical stage height to its 

normal position.

 

Note:   

  When raising the mechanical stage, do not 

make contact with the objective. 

3)  Turn the desired objective into light path. 

4)  Turn the annular ring disk to put the corresponding 

ring into light path, i.e. if you are using the 40x 
phase contrast objective, you should turn the disk 
at 40 as shown in 

Fig. 17

 

Fig.16 

  Iris

 

Ring 

20 

Fig.17 

Summary of Contents for MD827S30 series

Page 1: ...User Manual Digital Binocular Compound Microscope Model MD827S30 series MicroscopeNet com...

Page 2: ...1 Component Illustration 3 2 Installation 4 3 Operation 7 4 Specifications 10 5 Optional Parts 11 6 Troubleshooting Guide 13 7 Darkfield Condenser Installation and Operation Instructions 15 8 Phase C...

Page 3: ...cimen solutions or other liquids splash onto the stage objective or any other component disconnect the power cord immediately and wipe up the spillage Otherwise the instrument may be damaged 5 Importa...

Page 4: ...ng an excessive amount of solvents as this may cause problems with optical coatings or cemented optics or the flowing solvent may pick up grease making cleaning more difficult Oil immersion objectives...

Page 5: ...ody 21 Condenser Focus Knob 4 Nosepiece 13 Focus Tension Ring 22 Focus Stop Lever 5 Objective 14 Coarse Focus Knob 23 Abbe Condenser 6 Slide Holder 15 Fine Focus Knob 24 Color Filter Holder 7 Mechanic...

Page 6: ...mechanical stage is at its lowest position 2 Turn the caps counter clockwise to remove them from the nosepiece 3 Take the objectives out from the plastic cases and turn each one clock wise into the h...

Page 7: ...You may also loosen the two screws on the cover to adjust the position of the bulb to get centered and even brightness See Fig 3 6 Screw the cover on Caution Before you turn over the microscope be su...

Page 8: ...he hole at the center of the black disc See Fig 5 You may try to get reflected ambient light on either side of the mirror with different angles for best result Note The mirror is only used when there...

Page 9: ...tance is correct when the left and right fields of view converge completely into one image 3 4 Adjusting eyepiece diopter 1 Rotate the 10x objective into position 2 Rotate the diopter rings on the eye...

Page 10: ...t When you use the 100X objective to observe the specimen you have to finish observing the specimen with the 4X 10X 40X objectives When you use the 100X objective to observe the specimen you have to a...

Page 11: ...into the USB port Fig 11 on the back of viewing head and the other end to the computer 3 Turn on the computer install the camera following the manual in the mini CD 4 Open image observing software to...

Page 12: ...ing knobs on both sides w focus stop Minimum fine focusing adjustment at 0 002mm range 28mm Mechanical Stage Double layer Dimension 5 1 2 x 5 1 2 140mmx140mm Translational range 3 x 2 75mm X 50mm Came...

Page 13: ...kfield Condenser Numerical Aperture Objective Mounting Size diameter A191 Dry 0 7 0 9 37mm A191BOIL Oil 1 36 1 25 Plan 100X 1 25 oil 0 5 160 0 17 spring w iris diaphragm 37mm 2 Carrying Case Model Dim...

Page 14: ...hase plate Achromatic 40X with built in phase plate spring Achromatic 100X with built in phase plate spring oil five positions 10 for 10X phase contrast objective 20 for 20X phase contrast objective 4...

Page 15: ...too thick or thin Use a slide cover of the appropriate thickness 0 17mm Slide may be upside down specimen at the bottom Turn slide over so the cover glass faces up Diopter adjustment is not set proper...

Page 16: ...e condenser is not in the right position Lower or raise the condenser slightly to the position that the specimen is the brightest A191BOIL only Make sure the oil contact the bottom of the slide and th...

Page 17: ...e condenser up or down slightly to get the best darkfield viewing Note The dry darkfield condenser is used with the dry objectives only The dry darkfield condenser works with the 4X 10X 40X objectives...

Page 18: ...Install the phase contrast objectives onto the nosepiece following the steps in 2 3 2 Mounting the phase contrast condenser annular ring disk a Rotate the nosepiece and set the 4x objective in positio...

Page 19: ...k being put at B position 6 Performing the phase contrast observation After centering the condenser ring plate perform the phase contrast observation the same way as a normal bright field microscope N...

Page 20: ...cope d Observe from the telescope The bright ring and dark ring should be coincided with each other as shown in Fig 21 d e If the ring images are not clear turn the top of telescope until both ring im...

Page 21: ...ptional When you apply the immersion oil with the 100X objective do not let the immersion oil to contact with the dry objective lenses especially the 40X If the immersion oil is on the dry objectives...

Page 22: ...is hard to find put a normal white print paper on the stage and under the objective then you will see the dark ring as shown in Fig 25 a h If the bright ring is still obscure as in Fig 25 b raise or l...

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