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AX motorized focusing unit AX-FOM

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AX motorized transmitted field stop unit AX-TFSM

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AX motorized transmitted filter changer AX-TFCHM

4

AX motorized universal condenser AX-UCDM

Motorized unit

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FISH and Fluorescence advantages

The PROVIS AX70 thoroughly addresses the concerns of Fluorescence
and Fluorescence In Situ 
Hybridization (FISH) microscopy.

UIS objectives produce fully corrected primary images.  This leads to 
exceptional RGB image registration for high resolution CCDs.

High N.A. fluorescence optimized Fluorite and Plan Apochromat objectives
deliver images of exceptional brilliance, contrast and resolution.

The wide choices of filter systems satisfies every fluorescent label and 
includes filter cubes for simultaneous multi wavelength excitation and 
emission.  In addition, filter systems can be tailored to future demands.

Ready adaptability of filter wheels to the massive stand enables the use of 
stationary multichroic beam-splitter emission filters with single, dual and triple
wavelength exciter.  This approach renders the highest registration 
accuracy.

The chromatically corrected illumination beampath produces uniform
fluorescence over a wide field of view, encompassing the entire excitation
emission spectrum.

Efficient heat and IR removal in the collector lens protects interference exciter
filters from unnecessary energy.

The automation capability (RS232C/GPIB) of the PROVIS AX70 makes it an
ideal base for automated FISH imaging stations.

The combination of motorized units
with a multi-control box automates
the entire microscopic procedure. 

A motorized universal condenser allows
quick, simplified changeover between

AUTOMATIC

OPERATION

Chromosome – 1, 4 and 9 in human metaphase Chromosomes/

Spectrum FITC+DAPI/

UPLAPO 100

×

different microscopic methods.  The
AX70 also includes a motorized filter
changer and motorized revolving
nosepiece to speed selection of
excitation wavelength.   In addition, the
AX70 allows the operator to affect
setting changes.  This is achieved
through the motorized field stop and
aperture stop units, and the motorized
filter changeover, which can be used to
perform various microscopic
illumination techniques automatically.
The use of a multi-control box may
automate many functions including
photomicrography. 

A computer-based control system.

For more advanced features, the multi-
control box can be linked to an external
computer via an interface.  A computer
control system can therefore be
configured for other ancillary equipment
connected for use in FISH, patch
clamping and other biological research
applications.  Remote controlled
photomicrography operation is also
possible.

Summary of Contents for PROVIS AX70

Page 1: ...UNIVERSAL INFINITY SYSTEM RESEARCH SYSTEM MICROSCOPE AX70 ...

Page 2: ...1 Thereferenc for future mi Essential research equipment ...

Page 3: ...exibility to accommodate tomorrow s research needs To date only one microscope fulfills all these demands the AX70 Research Microscope from Olympus Offering uncompromising performance and outstanding system integration potential the AX70 is a reference standard for future microscopes PROVIS stands for PROfessional VISion WORLD CLASS OPTICS ILLUMINATION VERSATILITY HIGH STABILITY USER COMFORT AUTOM...

Page 4: ... adjustment for the objective in use as well as automatic observation light path selection Flexible moving spot measurement ensures optimum exposure regardless of specimen distribution A built in Super FL Auto mode offers fully automatic exposure control for fluorescence photomicrography And easy flexible framing is assured via the zoom magnification system By centralizing operation with a multi c...

Page 5: ...microscopic methods And the system s substantial frame accepts heavy attachments for image processing and photometry applications These and many other advanced features boost the performance of the AX70 and ensure flexible system upgradability Olympus further maximizes system versatility with various accessories including the AX REXBA to optimally balance fluorescence for double stained fluorescin...

Page 6: ...The Olympus UIS optics The new standard of excellence 5 ...

Page 7: ...es ideal for 3D observations with a confocal laser scanning microscope With improved near ultraviolet transmission these objectives can also be used for Ca2 photometry UAPO W Series The UAPO W water immersion objectives feature a high numerical aperture Thus they enable the production of aberration free images at every specimen level from the surface down to deep sections during examination of liv...

Page 8: ...7 Maximum quality for various microscopy applications ...

Page 9: ...eo Enhanced Contrast and metallurgy are also available Design algorithms suited for polarized light observation The entire UIS optical system including high precision strain free POL objectives condensers and observation tube prisms has been designed to minimize reflections from lens surfaces and frame Together with dedicated Olympus optical design algorithms this ensures unmatched image contrast ...

Page 10: ...9 A range of illuminator systems for advanced research applications ...

Page 11: ...ral fluorescent indicators These range from 1 wavelength excitation 1 wavelength photometry 1Ex1Em to 2 wavelength excitation 2 wavelength photometry 2Ex2Em Thus the AX70 OSP100 combination can measure fluorescence intensities at a single wavelength with dual wavelength excitation using the fura 2 or BCECF indicator These advantages make it ideal for analyzing intracellular calcium ion concentrati...

Page 12: ...11 Structural integrity for vibration resistance weighty accessories and thermal stability ...

Page 13: ...lity the power supply was removed from the body of the microscope The AX70 was also given a heat insulating structure to reduce the temperature influence of the lamp housing on the frame These measures effectively minimize heat induced frame flexure on the AX70 HIGH STABILITY Application example Video Enhanced Contrast A low contrast sample unidentifiable in ordinary microscopic observation can be...

Page 14: ...Maximum operator comfort at all times 13 ...

Page 15: ...s The AX70 also has enough space between the stage surface and arm to easily accommodate large and thick specimens Improved operational ease for enhanced manipulation The revolutionary Y shape of the AX70 frame offers unique user benefits It not only allows the operator s forearms to be supported by the worktable or bench surface but also provides more working space As a result micromanipulators c...

Page 16: ...Automation facilitates research microscopy 15 ...

Page 17: ...ion emission spectrum Efficient heat and IR removal in the collector lens protects interference exciter filters from unnecessary energy The automation capability RS232C GPIB of the PROVIS AX70 makes it an ideal base for automated FISH imaging stations The combination of motorized units with a multi control box automates the entire microscopic procedure A motorized universal condenser allows quick ...

Page 18: ...A 1 2 zoom magnification change system significantly eases zooming and trimming of a desired section to significantly increase framing options flexibility for video recording U PHOTO Universal Photo System The U PHOTO Universal Photo System is an automatic photomicrography system that provides fully automatic functions for photomicrography These include a flexible spot measurement function a Super...

Page 19: ...s The focusing speed during ordinary brightfield observation has been raised significantly to achieve focus in only 0 3 2 seconds In addition to the one shot mode a real time mode is included to maintain specimen focus and significantly enhance observation efficiency even during objective and microscopic technique changeovers or specimen movement The Auto focus function can be activated by inserti...

Page 20: ...AX REXBA allows adjustment of excitation light unnecessary irradiation is prevented and damage to the specimen is minimized 3 The 25EBFB G filter accommodates a double stained fluorescing specimen using two types of fluorochromes FITC TRITC and FITC Texas Red This filter is designed for general use with double stained specimens with G B excitation The 25EBFU B filter designed for U B excitation is...

Page 21: ...C TRITC UPLAPO40 PE3 3 Optical center adjuster AX OCA The AX OCA effectively eliminates the deviations during multiple exposure Specimens viewed with AX REXBA TRITC enhanced FITC enhanced Precise correction for image deviation in multiple exposure ...

Page 22: ... selector Observation tubes The AX Series observation tubes range from widefield binocular to super widefield erect image trinocular corresponding to various observation needs PM30 automatic photomicrography system The exclusive Super FL Auto mode provides fully automatic exposure control for effortless fluorescence photo micrography The Auto mode accommodates all other observation methods and a h...

Page 23: ...path 80 of all light goes to camera optical path during exposure BI video camera direct video camera 2 photometry 20 0 80 0 100 0 0 0 0 100 0 0 20 0 0 80 0 20 0 80 video camera 2 is mounted to large format port via U TVL Camera optical path 35mm left large format 35mm right 100 0 0 0 100 0 0 0 100 When using video camera 2 35mm left 35mm right 100 0 0 100 Photo magnification 35mm camera 2 5x 5x la...

Page 24: ...out any obligation on the part of the manufacturer ISO9001 Certification Design and production adheres to ISO9001 international quality standard ISO 9001 Accredited by the Dutch Council for Accreditation ISO14001 Design and production at the Olympus Optical Co Ltd Ina Plant conforms with ISO14001 specifications for environmental management systems Certification ISO 14001 008 U K A S ENVIRONMENTAL ...

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