VARIETIES OF OBSERVATION METHODS
/ Visual observation (IX)
II.
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4-2 Visual observation (IX)
This system can be used for not only reflected-light fluorescent observation but also it combines
transmitting bright-field observation, transmitted light phase contrast observation and transmitting
differential interference observation. Particularly, for specimen that color deterioration occurs earlier,
the color deterioration can be minimized by initial positioning through phase contrast observation or
differential interference observation. In addition, simultaneous observation of reflected-light
fluorescent combined with phase contrast observation or reflected-light fluorescent combined with
differential interference observation is possible, and it enables to observe which part of cell is emitting
fluorescence plainly.
IX81 or IX71 provides lamp ON-OFF button. Use these buttons when you change to fluorescent
observation only.
4-2-1 Simultaneous observation of reflected-light fluorescence and phase
contrast
For phase contrast observation, any one of condenser IX2-LWUCDA, IX2-
LWUCD, IX-ULWCD or U-UCD8 and objective lens for phase contrast are
required.
1. Put dummy mirror unit (the place where fluorescent mirror unit is not installed) into
light path.
2. Set Ph indication equivalent to Ph indication of objective lens on phase contrast
turret.
3.
Do centering between ring slit and phase plate.
4.
Put mirror unit to observe excitation desired into light path and open shutter.
5. Adjust transmitting light intensity to get optimum brightness balance for fluorescent
and phase contrast and then, do the observation.
To adjust transmitting light intensity, use ND filter or green filter.
Alternatively, adjust brightness at microscope side.
Moreover, be careful that, if halogen lamp were used at low voltage
applied at lamp off time, the lamp is susceptible to shorten its life.
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