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Olympus BX51-P, Instructions Manual, Page: 3 / 36

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Olympus BX51-P Instructions Manual Download Page 3

IMPORTANT 

— Be sure to read this chapter for safe use of the equipment. —

 1

BX51/52-P,BX41-P

CONTENTS

1 NOMENCLATURE

  2

2 ASSEMBLY

  3-9

2-1 Assembly Diagram

.........................................................................................................................................................................................................................................

3

2-2 Detailed Assembly Procedure

................................................................................................................................................................................................

4-9

3 CONTROLS

  10-11

4 USING THE CONTROLS

  12-15

4-1 Stage

.........................................................................................................................................................................................................................................................................

12-14

4-2 Intermediate Polarizing Attachment

......................................................................................................................................................................................

15

5 POLARIZED LIGHT OBSERVATION

  16-26

5-1 Adjustments Before Observation

....................................................................................................................................................................................

16-24

5-2 Orthoscopic Observation

.....................................................................................................................................................................................................................

25

5-3 Conoscopic Observation

......................................................................................................................................................................................................................

26

6 SPECIFICATIONS

  27-28

7 OPTICAL CHARACTERISTICS

  29

8 TROUBLESHOOTING GUIDE

  30

Summary of Contents for BX51-P

Page 1: ... To obtain optimum performance and to familiarize yourself fully with the use of your microscope we recommend that you read this manual together with the instruction manual for the BX51 BX52 BX41 microscope thoroughly before use Retain this instruction manual in an easily accessible place near the work desk for future reference A X 7 3 4 4 ...

Page 2: ......

Page 3: ...2 2 Detailed Assembly Procedure 4 9 3 CONTROLS 10 11 4 USING THE CONTROLS 12 15 4 1 Stage 12 14 4 2 Intermediate Polarizing Attachment 15 5 POLARIZED LIGHT OBSERVATION 16 26 5 1 Adjustments Before Observation 16 24 5 2 Orthoscopic Observation 25 5 3 Conoscopic Observation 26 6 SPECIFICATIONS 27 28 7 OPTICAL CHARACTERISTICS 29 8 TROUBLESHOOTING GUIDE 30 ...

Page 4: ...ONS on page 28 If the microscope is used in a manner not specified by this manual the safety of the user may be imperiled In addition the equipment may also be damaged Always use the equipment as outlined in this instruction manual 1 Getting Ready 2 Caution The following symbols are used to set off text in this instruction manual Indicates that failure to follow the instructions in the warning cou...

Page 5: ...ttachment Centerable revolving eyepiece Polarizing rotatable stage or Rotatable stage Observation tube BX51 52 Microscope frame or BX41Microscope frame Objective Strain free objective series Lamp housing Components for polarized light observation Strain free condenser ...

Page 6: ...ng glass surfaces 2 1 Assembly Diagram Eyepiece Centerable revolving nosepiece U P4RE Test plate adapter U TAD Sensitive tint plate U TP530 Quarter wave plate U TP137 Strain free objective Mechanical stage U FMP Stage clips Polarizing rotatable stage U SRP Rotatable stage U SRG2 Polarizing intermediate attachment U CPA Observation tube Intermediate attachment for AN360P U OPA Rotatable analyzer U ...

Page 7: ...acket then loosely tighten the centering knobs When using the U SRG2 Using the Allen screwdriver provided with the microscope frame loosen the clamping screw on the front of the stage Then after lowering the stage onto the stage bracket tighten the clamping screw Mounting Stage Clips and Mechanical Stage U FMP Fig 2 Insert the stage clips securely into any two holes ƒ on the stage top surface Moun...

Page 8: ...crew 4 Position the condenser with the scale markings in front insert it into the substage fork as far as it will go Align the positioning pin at the back of the condenser with the groove in the substage fork Swing the top lens out of the way before inserting the condenser 5 Tighten the condenser clamping screw then raise the condenser to its upper limit 2 Mounting the Condenser U POC 2 Fig 3 Fig ...

Page 9: ...r in a clock wise direction from the primary hole 4 Mounting the Revolving Nosepiece U P4RE Fig 5 1 Turn the coarse adjustment knob to lower the stage all the way 2 Using the Allen screwdriver loosen the nosepiece clamping screw on the microscope frame 3 Carefully slide the nosepiece along the dovetail to its limit in the direction of the arrow 4 Clamp the nosepiece by tightening the nosepiece cla...

Page 10: ...ate tube can be adjusted later At this point place the tube so the nameplate is toward the rear 1 Using the Allen screwdriver loosen the observation tube clamping screw on the microscope frame 2 Insert the circular dovetail mount at the bottom of the intermediate attachments into the opening on the microscope frame and clamp by tightening the clamping screw Fig 6 5 Mounting the Intermediate Attach...

Page 11: ...sten the observation tube by tightening the clamping screw 7 Mounting the Eyepieces Insert the eyepiece with cross lines into the right hand eyepiece sleeve Make sure the eyepiece is inserted so that the positioning pin on the eyepiece is inserted into the groove at the lower end of the sleeve 1 Loosen the revolving nosepiece clamping knob and remove the dummy slider 2 Insert the test plate adapte...

Page 12: ...the test plate adapter U TAD Position the fixed analyzer so that the positioning pin on the fixed analyzer fits into the groove The fixed analyzer will be held in place by a magnet 1 Loosen the test plate adapter U TAD clamping knob 2 Insert the sensitive tint plate U TP530 quarter wave plate U TP137 or any of the other compensators 6 types into the test plate adapter and securely tighten the clam...

Page 13: ...intro duced on the following pages Bertrand lens knob CONTROLS Slider clamping knob Mechanical stage Vernier scale Aperture iris diaphragm lever Polarizer rotation ring Bertrand lens focusing knob Analyzer Slot for compensators Stage rotation clamping knob Polarizer clamping knob ...

Page 14: ...Stopper knob Rotation dial Clamping knob Test Plate Adapter U TAD Test plate clamping knob Hole for the fixed analyzer U ANT Slot for compensator plate Sensitive Tint Plate U TP530 Quarter Wave Plate U TP137 U FMP mounting screw holes Centering knob Stage clips Stage rotation clamping knob Stage center plate Rotatable Stage U SRG2 Clamping screw ...

Page 15: ...e center and clamp the specimen with the stage clips When Using the Mechanical Stage U FMP Fig 12 Open the spring loaded finger and place the specimen on the stage Applicable slide glass is for inorganic substances 28 x 48 mm and organic substances 26 x 76 mm with cover glass thickness of 0 17 mm Inorganic specimen Organic specimen Fig 11 ...

Page 16: ...er located at the right side of the stage is moved toward the observer and the stage is moved from this position to the first click stop position the specimen is moved 45 to its diagonal position To release the 45 click stop function push back the lever When releasing the 45 click stop function by pushing back the lever do so at a click stop position Fig 13 2 Stage Rotation Fig 13 ...

Page 17: ... page 4 2 Using the Allen screwdriver loosen the substage bracket clamping screw and remove the substage assembly Fig 15 3 Turn the coarse adjustment knob and raise the focusing block to where the stopper screw in the arm becomes visible Fig 16 4 Using the Allen screwdriver loosen and remove the upper stopper screw 5 Reattach substage assembly and stage Store the remove stopper screw in a safe pla...

Page 18: ...alyzer Fig 18 The analyzer is engaged when pushed in to the second click stop position To disengaged the analyzer and to place the empty hole in the light path pull the analyzer out to the first click stop 3 Focusing the Conoscopic Image U CPA only Fig 18 To focus the conoscopic image manipulate the Bertrand lens focusing knob While observing the conoscopic image move the knob to find the position...

Page 19: ...ake sure the 10X objective is inserted into the primary hole on the centerable revolving nosepiece 1 Fully insert the alignment polarizer provided with the U OPA into the test plate adapter U TAD Tighten its clamping knob Fig 19 2 Remove the condenser 3 Insert the rotatable analyzer U AN360P 2 into the analyzer slot on the intermediate attachment Engage the analyzer loosen the clamping screw and s...

Page 20: ...ge Figs 21 22 1 Place the specimen 2 Focus on the specimen and look for an easily recognizable detail in the field Move this detail in the center of the eyepiece cross lines 3 When the stage is rotated the detail moves in circle Manipulate the two stage centering knobs to coincide the imaginary center of the circle circumscribed by the detail with the intersection of the eyepiece cross lines 4 Mov...

Page 21: ...nd lens knob to the OUT position Fig 23 2 Center the condenser Refer to page 18 in the BX51 52 instruction manual or page 16 in the BX41 instruction manual 3 Follow steps 9 and 10 in When Using the Intermediate Attachment for AN360 Fig 22 10 This concludes centering of the optical axis for the 10X objective which will act as the reference objective Now center the other objectives with the centerab...

Page 22: ...ed in the following paragraph When Using Polarizing Intermediate Attachment U CPA Photomicrography 1 Using the tip of a mechanical pencial etc push out in the direction of the objective hole the black rubber plugs inserted into the centering holes of the primary hole where the 20X or 10X objective is inserted 2 Pull out the Bertrand lens knob to the OUT position Fig 25 3 Adjust the position of the...

Page 23: ...g 26 7 Insert the two provided centering wrenches into the centering holes for the primary hole with the 20X or 10X objective on the nosepiece 8 Manipulate the centering wrenches to bring the bright center portion of the conoscopic image into the center of the field of view Fig 27 9 Pull out the Bertrand lens knob to the OUT position At this state center the condenser again as in step 4 above Fig ...

Page 24: ...ecentration this will move the specimen detail in the opposite direc tion away from the cross line center 4 Moving the specimen only move a new specimen detail into the cross line center Repeat steps 3 and 4 several times until the center of the stage rotation is in the center of the cross lines i e when rotating the stage the specimen detail stays in the center of the cross lines 5 Clamp the stag...

Page 25: ...rotatable analyzer into the light path and set vibration direction scale at the 0 position Clamp with the clamping knob Fig 30 3 Set the polarizer scale at the 0 position 4 Loosen the polarizer clamping knob Rotate the polarizer rotation dial until complete extinction is obtained At this point tighten the clamping knob Fig 30 Fig 30 2 Adjusting for Extinction Fig 30 ...

Page 26: ...uired Orientation plate U PJ Single module name Remove the test plate adapter and the compensator from the light path 1 Swing out the top lens of the condenser 2 Place the orientation plate on the stage Remove the analyzer from the light path and use the 4X objective to focus Fig 31 3 Adjusting the Eyepiece Cross Lines Fig 31 ...

Page 27: ...bserving rotate the stage to locate the position where the orientation plate is darkest At this point clamp the stage 5 Remove the analyzer from the light path Set to brightfield and slightly loosen the observation tube clamping screw 6 Rotate the observation tube to position the eyepiece cross line parallel to the fiducial line of the orientation plate Tighten the observation tube clamping screw ...

Page 28: ...and lens from the light path 2 Insert the analyzer and perform observation When the top lens is swung out the aperture iris diaphragm and field iris diaphragm will not function normally If the aperture iris diaphragm is stopped down the field of view may be restricted 3 Insert a test plate sensitive tint plate U TP530 quarter wave plate U TP137 into the test plate slot When fully inserted the plat...

Page 29: ...gm 6 Slide the focusing knob on the U CPA to focus on the conoscopic image When the polarizing intermediate attachment U CPA is not used conoscopic observation is possible by removing an eyepiece from the observation tube and looking at the rear focal plane of the objective directly To obtain good image contrast place an interference filter 45IF546 in the filter holder on the light exit of the mic...

Page 30: ...zer U AN360P 2 3 Revolving nosepiece U P4RE 360 dial rotation Minimum scale reading 0 1 Vernier scale Type Centerable quadruple Attachable compensators Quarter wave plate U TP137 sensitive tint plate U TP530 and all kinds of compensators by means of test plate adapter U TAD 4 Stage U SRP Type Polarizing rotatable stage with 3 point centering mechanism 360 horizontal rotation clamps at any desired ...

Page 31: ...88 F decreasing linearly to 50 relative humidity at 40 C 104 F Main supply voltage fluctuations not to exceed 10 of the nominal voltage Installation Overvoltage Category II In accordance with IEC60664 Pollution Degree 2 In accordance with lEC60664 5 Stage U SRG2 Type Rotatable stage with 3 point centering mechanism 360 horizontal rotation clamps at any desired position 360 scale minimum division 1...

Page 32: ...ective Total mag Depth of focus µm Field of view PlanN P Plan Achromat for polarized light FN22 AchN P Achromat for polarized light FN22 The table below shows the optical characteristics of different eyepiece and objective combinations Objective specifications are marked on the objective as shown in the diagram on the right NOTE Refer to the latest catalogue or consult your local Olympus represent...

Page 33: ...yzer from the light path b Vignetting or uneven field of view Test plate is stopped at an intermediate position Set at click stop position During orthoscopic observation the con denser top lens is placed in the light path or stopped at intermediate position Remove top lens completely from the light path c The conoscopic image is not visible Condenser top lens is not placed in the light path Swing ...

Page 34: ...MEMO ...

Page 35: ......

Page 36: ...Drive Orangeburg NY 10962 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 2 8 Honduras Street London EC1Y OTX United Kingdom 31 Gilby Road Mt Waverley VIC 3149 Melbourne Australia 6100 Blue Lagoon Drive Suite 390 Miami FL 33126 2087 U S A This publication is printed on recycled paper Printed in Japan 2004 09 M 010 ...

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