4
General specifications
Microscopy techniques available
Brightfield
Phase contrast
Epitluorescence
Objectives
O ly mpu s UP l an FL N 10x Ph 1 NA 0. 3 WD 10. 0 mm
Olympus LUCPlanFLN 20x Ph1 NA 0.45 WD 6.6-7.8 with correction collar
Olympus LUCPlanFLN 40x Ph2 NA 0.6 WD 3.0 -4.2 with correction collar
Ze is s o bj ec t ive s c an al s o be use d
Filter cubes (see p11-15)
Position Filter set name
Exciter
Beamsplitter Emitter
1
DAPI
350/50x
400LP
ET460/50m
2
GFP
ET470/40x
495LP
ET 525/50m
3
DsRed (TRITC/Cy3) ET545/30x
T570LPXR
ET620/60m
4
Cy5
ET620/60x
T660LPXR
ET700/75m
5
Cy7
ET710/75x
T760LPXR
ET810/90m
6
empty (brightfield)
Camera (see p16)
Hamamatsu ORCA R
2
CCD Camera with HClmage Capture Software
Fluorescence illumination (see p16)
Prior Lumen 200 Metal Halide Light Source (2000 hours/bulb)
Pixels to Microns calibration
5x objective binning 1x1
1 pixel = 1.88 um
10x objective binning 1x1
1 pixel = 1.03 um
20x objective binning 1x1
1 pixel = 0.514 um
40x objective binning 1x1 1 pixel = 0.256 um
Calibration with a micrometer under transmitted white ligh t
Consumables list
Price correct as of November 2013
Prior Lumen 200 bulb LM375 (£550, Prior Scientific Instruments Ltd)
Prior Lumen 200 light guide LM587 (£400, Prior Scientific Instruments Ltd)
Halogen bulb 12V/100W (£1.8, Technical Lamp Supplies UK)
Summary of Contents for 1X71
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