Okolab H301-NIKON-TI-SR User Manual Download Page 1 | Manualshive

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H301-NIKON-TI-SR

User Manual

March 2015

www.oko-lab.com

Pa

ge

1

H301-NIKON-TI-SR

Compatible with the following XY stages

Compatible with the following Okolab Controllers



Nikon T/Ti-SR



H301-T-BL-PLUS



UNO-COMBINED-CONTROLLER



H401-T-DUAL

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2
3
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Summary of Contents for H301-NIKON-TI-SR

Page 1: ... User Manual March 2015 www oko lab com Page 1 H301 NIKON TI SR Compatible with the following XY stages Compatible with the following Okolab Controllers Nikon T Ti SR H301 T BL PLUS UNO COMBINED CONTROLLER H401 T DUAL ...

Page 2: ... Injection Lid 6 3 4 Laser Interlock Lid 7 4 Insertion of the Sample Feedback Temperature Sensor 8 5 Insertion of Sample Holder into Chamber 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 9 7 Working with 1x3 and 1x2 chamber slides magnetic locks 11 8 Connecting the Gas Supply and Filling the Humidity Module 12 9 Working with Perfusion 12 10 Connecting the Chamber with XY...

Page 3: ...e of the glass lid Chamber top view Sliding Lid Chamber Base Sample Holder Order Separately Integrated Humidty Module Figure 1 H301 NIKON TI SR Components and Dimensions 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the lis...

Page 4: ...ON TI SR H301 SLIDING LID For easy sample loading and pipetting Included in code H301 NIKON TI SR H301 KOEHLER LID 2 Reduces chamber height to 23 mm and allows imaging under Koehler illumination H301 INJECTION LID 2 Heated glass lid with two small openings sealed with flexible plastic allowing injec tion or permanent access to the sample Compatible only with sample holder 1x35 H301 LASER INTERLOCK...

Page 5: ...is necessary NOTE Sliding Lid MUST BE REMOVED when using any other Lid The Sliding Lid is fixed onto the chamber with 4 screws Screws location is indicated by letter A in Figure 4 image 2 and 3 Keep Sliding Lid fully closed to access screws labeled A in image 2 Open Sliding Lid to access screws labeled A in image 3 Distance between focal plane and upper side of the glass lid Chamber top view Slidi...

Page 6: ...nsions with Koehler lid Distance between focal plane and upper side of the glass lid Chamber top view H301 KOHLER LID 2 Figure 5 H301 NIKON TI SR H301 KOEHLER LID 2 3 3 Injection Lid The Injection Lid is a heated glass lid with two small openings sealed with flexible plastic allowing injection or perma nent access to the sample NOTE Compatible with sample holder 1x35 ONLY The Injection Lid is plac...

Page 7: ...ass lid with a safety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid Distance between focal plane and upper side of the glass lid Chamber top view H301 L...

Page 8: ...emperature Sensor dedicated opening Figure 8 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figur...

Page 9: ...d 60 mm petri dishes Included in the corresponding sample holder Figure 10 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings with variable diameter are also included in according to the needs of the user Figure 11 shows how to position the ring inside the sample holder Ring selection guide diagrams rings for 35 and 60 mm dish Figure 12 and Figur...

Page 10: ... Ibidi μ Dish35mm low Corning 35x10mm MatTek P35G X 14 X Willco 35x10 BD Falcon 35x10 Figure 12 Ring selection scheme for 35 mm dish MatTek P50G X 30 X Grainer Petri 60x15 Willco60x15 BD Falcon 60x15 Ibidi μ dish 50mm low Corning 60x15mm Figure 13 Ring selection scheme for 60 mm dish ...

Page 11: ...s Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 2 1 Figure 14 Magnetic lock for 1 x 3 chamber slide 1 3 2 Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 ...

Page 12: ...y percentage inside the chamber Fill the humidity module inside the chamber through the water input brass opening located on a side of the H301 NIKON TI SR See Figure 16 using a silicon tubing 2 mm ID and a syringe NOTE Do not exceed 0 05 l Gas Input Water Input Figure 16 Connection with gas supply 9 Working with Perfusion H301 NIKON TI SR features 8 perfusion holes for the insertion of perfusion ...

Page 13: ...e chamber with XY stage 1 Place the chamber on the stage and tighten 4 captive screws See Figure 18 Image 1 and Image 2 Captive screws housings are indicated with letter B in Figure 18 Image 2 2 Use a 2 5mm metric Allen Wrench to tighten the four captive screws while keeping the chamber sliding lid open See Figure 18 Image 2 Captive Screws A B 1 2 3 Figure 18 Connection of the Chamber with XY Stag...

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