Okolab H301-LUDL 96A602 User Manual Download Page 1

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H301-LUDL 96A602

User Manual

Rev 03

www.oko-lab.com

H301-LUDL 96A602

Compatible with the following Z piezo stage

Compatible with the following Okolab Controllers

•

Ludl 96A602

•

H301-T-BL-PLUS

•

UNO-T

•

UNO-T-H-PREMIXED

•

UNO-T-H-CO2

Summary of Contents for H301-LUDL 96A602

Page 1: ...02 User Manual Rev 03 www oko lab com Page 1 H301 LUDL 96A602 Compatible with the following Z piezo stage Compatible with the following Okolab Controllers Ludl 96A602 H301 T BL PLUS UNO T UNO T H PREMIXED UNO T H CO2 ...

Page 2: ...Feedback Temperature Sensor 7 5 Insertion of Sample Holder into Chamber 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks 9 7 Working with 1x3 and 1x2 chamber slides magnetic locks 11 8 Working with MW Plates Magnetic locks and Chamber riser 12 9 Connection of the Gas Supply 12 10 Working with Perfusion 13 10 1 MICROFLUIDIC C RISER Optional 15 11 Connection of the Chamber wi...

Page 3: ...iser Chamber top view KOEHELER LID Chamber Riser Chamber Base Sample Holder Order Separately Figure 1 H301 LULD 96A902 Components and Dimensions 2 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1x35 M 1 35mm Petri dish 1xGS M 1 1x3in ch...

Page 4: ...n chambered cover glass and 1 60mm Petri dish GS60 M 1 1x3in chamber slide and 1 60mm Petri dish 6035 M 1 60mm Petri dish and 1 35mm Petri dish 6MW 6 well plates 12MW 12 well plates 24MW 24 well plates 24MW NUNC 24 well NUNC GREINER plates 48MW 48 well plates 96MW 96 well plates Includes MW OIL MW OIL Open frame to image multi well plates with oil immersion objectives Figure 2 Available sample hol...

Page 5: ...ompatible only with sample holder 1x35 OPTIONAL not included with H301 LUDL 96A602 3 1 Koehler Lid The Koehler Lid is a heated glass lid and allows imaging under Koehler illumination The Koehler Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 3 shows chamber dimensions with Koehler lid with and without chamber riser Distance between focal plane and upper side of the glass li...

Page 6: ...ween focal plane and upper side of the glass lid without the chamber riser Distance between focal plane and upper side of the glass lid with the chamber riser Chamber top view H301 INJECTION LID Figure 4 H301 LUDL 96A902 H301 INJECTION LID 3 3 Laser Interlock Lid The Laser Interlock Lid is a heated glass lid with a safety switch connecting to the laser controller It automatically turns the laser o...

Page 7: ...p view H301 LASER INTERLOCK LID Figure 5 H301 LUDL 96A902 H301 LASER INTERLOCK LID 4 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H301 LUDL 96A602 see Figure 6 Frontal and 3D views 1 Frontal view 2 3D view Temperature Sensor dedicated opening Temperature Sensor dedicated opening Figure 6 Insertion of ...

Page 8: ...e and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 7 Insert the adapter by superimposing the red points Figure 7 Introduction of the Specimen Holder inside the Chamber Base ...

Page 9: ...agnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 9 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 10 and Figure 11 respectively 1 3 2 Figure 9 1 Insertion of the ring 2 insertion of the 35 mm dish...

Page 10: ... 35x10 Grainer Petri35x10 Ibidi μ Dish 35 mm low MatTek P35G X14 X Corning 35x10 mm Figure 10 Ring selection scheme for 35 mm dish Corning 60x15 mm BD Falcon 60x10 Ibidi μ Dish 50 mm low Grainer Petri 60x15 MatTek P50G X30 X Willco 60x15 Figure 11 Ring selection scheme for 60 mm dish ...

Page 11: ...Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 12 Magnetic lock for 1 x 3 chamber slide 1 2 3 Figure 13 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 ...

Page 12: ...UIRED with 6 12 24 48 MW plates regardless of the Lid em ployed Figure 15 shows how to mount and screw the chamber riser Screws location is indicated by letter A A Chamber Riser A Chamber Base Figure 15 Riser Assembly 9 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 LUDL 96A9602 Silicon tubing connects to a gas input brass opening...

Page 13: ...ertion of perfusion tubing up to 2 5 mm in outer diameter To lock the Chamber Riser into the base use a 1 5mm Allen key to secure the 4 GUTB2 2 5 6 screws as shown in Figure 17 Figure 17 How to lock the Chamber Riser Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws with a 1 5 mm Allen key as necessary before introducing perfusion tubing Figure 18 shows loc...

Page 14: ...H301 LUDL 96A602 User Manual Rev 03 www oko lab com Page 14 1 Frontal view 2 3D view Perfusion dedicated opening Figure 18 Perfusion Figure 19 How to access the perfusion holes ...

Page 15: ...ith 19x3 mm windows on the two short sides to allow insertion of microfluidic tubings Windows are magnetically closed Compatible with most of H201 and H301 Okolab chambers Thickness of this chamber riser is 8mm 2mm more than default chamber riser Requires Koehler lid and single ac commodation specimen holders Figure 20 MICROFLUIDIC C RISER ...

Page 16: ...x the chamber on the stage follow the steps shown in Figure 21 1 Place the chamber on the stage See Image 1 of Figure 21 2 Incline the chamber and slide it in direction of the springs until it snaps into the housing See Image 2 of Figure 21 3 Image 3 of Figure 21 shows the chamber fitted on the Z piezo Stage Spring 1 2 3 Figure 21 Connection of the Chamber with Z piezo Stage ...

Page 17: ...he chamber cools down To clean the body and the glass lid of the chamber wipe with a soft micro fiber cloth For stubborn smudges you can damp the soft micro fiber cloth with ethyl alcohol product code UN1170 Do not put any liquid di rectly on the chamber While cleaning the glass lid do not apply strong force to the surface of the glass lid because it can be damaged H301 LUDL 96A602_User Manual Rev...

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Okolab H301-LUDL 96A602, User Manual

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