Okolab H301-KEYENCE-BZX User Manual Download Page 1 | Manualshive

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User Manual

H301-KEYENCE-BZX

IST 2792_REV01

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Summary of Contents for H301-KEYENCE-BZX

Page 1: ...User Manual H301 KEYENCE BZX IST 2792_REV01...

Page 2: ...INSERTION OF THE SAMPLE FEEDBACK TEMPERATURE SENSOR 12 7 CLEANING 13 8 FIGURE LIST 13 9 DOCUMENT REVISION TABLE 13 1 Chamber Compatibility H301 KEYENCE BZX is a heated top stage chamber designed for...

Page 3: ...M 1 60mm Petri dish 1xT25 M 1 Nunc and Greiner T25 flask 2x35 M 2 35mm Petri dish 2xGS M 2 1x3in chamber slides 2xLABTEK M 2 Lab Tek 1in x2in chambered cover glass 2xLABTEK II M 2 Lab Tek II 1in x2in...

Page 4: ...UNC GREINER plates 48MW 48 well plates 96MW Holder to image 96 well plates with LD objectives MW OIL Holder to image 6 12 24 48 96 well plates with immersion objectives Figure 2 Available Sample Holde...

Page 5: ...nto Chamber Base Sample holders fit into the chamber base and are held in place by magnets embedded within both the chamber and the holder To introduce a sample holder with the proper orientation matc...

Page 6: ...es Threaded magnetic posts allow adjusting the holder s height Magnetic Locks for 35 and 60 mm petri dishes Included in the corresponding sample holder Figure 5 Magnetic Locks for 35 and 60mm dish Not...

Page 7: ...ded with sample holders Note Spacing rings do not block the petri inside its accommodation but they are designed to center the petri with respect to the observation circular window BD Falcon 35x10 Wil...

Page 8: ...Figure 8 Ring selection scheme for 60 mm dish 1 5 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder...

Page 9: ...hamber riser Magnetic locks hold MW plates in place into sample holder illustrated in Figure 11 Figure 11 Magnetic locks for MW plates Ships with 6 12 24 48 MW plates The Chamber Riser increases the c...

Page 10: ...tion A single silicon tubing carries output gas from the Okolab Gas Controller to the H301 KEYENCE BZX Silicon tubing connects to a gas input brass opening located on a side of the H301 KEYENCE BZX Se...

Page 11: ...10 in the chamber riser 13 in the chamber base on the sides for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use screws plug M3x0...

Page 12: ...e Figure 16 To read the temperature of the culture medium and realize a self calibration of the chamber fix the Temperature Sensor tip with adhesive tape on the bottom of the dish inside the H301 KEYE...

Page 13: ...mple Holder inside the Chamber Base 6 Figure 5 Magnetic Locks for 35 and 60mm dish 6 Figure 6 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock 7 Figure 7 Ring sel...

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