background image

H301-ALPHA3-SMALL 

User Manual 

   

REV 02

 

 

 www.oko-lab.com 

  

 

Pa

ge

5

 

4.

 

Working with perfusion 

The Chamber H301-ALPHA3-SMALL features 2 perfusion holes on the sides for the insertion of perfusion tubing up to 1 

mm in outer diameter. Follow the instruction below to work with perfusion: 

1.

 

Use the supplied Stainless Steel Perfusion Tubes. Attach a Silicon tubes O.D. 1,6mm I.D. 0,8mm (not supplied) to 

the Stainless Steel Perfusion Tubes (see image 1 in Figure 4)

2.

 

Insert the Stainless Steel Perfusion Tubes in the perfusion holes (see images 2 and 3 in Figure 4). 

Insert a Silicon tubes O.D. 1,6mm I.D. 0,8mm (not supplied) to the free end of the Stainless Steel Perfusion Tubes 

(see image 4 and 5 in Figure 4).  

 

 

Figure 4. Working with Perfusion 

 

 

 

 

Summary of Contents for H301-ALPHA3-SMALL

Page 1: ...ser Manual REV 02 www oko lab com Page 1 H301 ALPHA3 SMALL Compatible with the following microscope Compatible with the following Okolab Controllers PhaseView Alpha3 Light Sheet microscope Bold Line U...

Page 2: ...www oko lab com Page 2 Index 1 Components and dimensions 3 2 Insertion of the Sample Feedback Temperature Sensor 4 3 Connection of the Gas Supply 4 4 Working with perfusion 5 5 Installing the H301 ALP...

Page 3: ...ons H301 ALPHA3 SMALL includes the following components Objective Collar compatible with water immersion objective 21 mm diameter Sample Arm Cover Chamber Lid Chamber Base Chamber Base Objective Colla...

Page 4: ...ape on the sample holder inside the H301 ALPHA3 SMALL For more information about the self calibration please refer to the User Manual of the Okolab Controller in use Figure 2 Insertion of the temperat...

Page 5: ...with perfusion 1 Use the supplied Stainless Steel Perfusion Tubes Attach a Silicon tubes O D 1 6mm I D 0 8mm not supplied to the Stainless Steel Perfusion Tubes see image 1 in Figure 4 2 Insert the S...

Page 6: ...Note Please pay attention to the sample holder glass 4 Tighten the captive screw M2 to fix the chamber base around the sample holder by using the provided hexagonal key see image 2 in Figure 5 5 Place...

Page 7: ...H301 ALPHA3 SMALL User Manual REV 02 www oko lab com Page 7 5 4 2 1 3 7 6 Figure 5 Connection of the Chamber with Stage H301 ALPHA3 SMALL_User Manual Rev 02_Published in September 2020...

Reviews: