Okolab H201-UP User Manual Download Page 10

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H201-UP

User Manual

April 2015

www.oko-lab.com

Connection of the Gas Supply

A single silicon tubing carries output gas from the Okolab Gas Controller to the H201-UP. Silicon tubing connects to a

gas input - brass opening - located on a corner of the H201-UP. See Figure 12. Connect by gently pushing silicon tubing

onto brass opening.

Gas Input

Figure 12.Connection with gas supply.

Working with Perfusion

The H201-UP features 12 perfusion holes on the sides for the insertion of perfusion tubing up to 2.0 mm in outer di-

ameter. Small screws plug the perfusion holes when not in use. (

Grub screws M3x6

). Remove small screws as necessary

before introducing perfusion tubing.

Figure 13 shows the location of perfusion holes.

1.Frontal view

2.3D view

Perfusion dedicated opening

Figure 13. Perfusion

Summary of Contents for H201-UP

Page 1: ...al April 2015 www oko lab com Page 1 H201 UP Compatible with the following XY stages Compatible with the following Okolab Controllers XY stage model and serial number must be specified in purchase ord...

Page 2: ...1 Available Sample Holders 4 3 Insertion of the Sample Feedback Temperature Sensor 5 4 Insertion of Sample Holder into Chamber 5 5 Magnetic Locks 7 6 Working with 1x3 and 1x2 chamber slides magnetic l...

Page 3: ...pter the dimension and type of objective in use must be specified in the purchase order Holder the type of stage on which the chamber will fit must be specified in the purchase order Lid it features a...

Page 4: ...UP 1x35 M 1 35mm Petri dish UP 14xGS M 1 1x3in chamber slide UP 1xLABTEK M 1 Lab Tek 1in x2in chambered cover glass UP 1xLABTEK II M 1 Lab Tek II 1in x2in chambered cover glass UP 1x60 M 1 60mm Petri...

Page 5: ...t into the chamber base and are held in place by magnets embedded within both on the chamber and on the holder To introduce a sample holder with the proper orientation match the red dot on the holder...

Page 6: ...m Page 6 4 Remove the chamber by lifting it from workspace as shown in Figure 5 Images 5 and 6 5 Lift and replace the sample holder as shown in Figure 5 Image 8 4 1 3 6 5 2 A 7 Figure 5 Introduction o...

Page 7: ...the corresponding sample holder Figure 6 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings with variable diameter are also included in according t...

Page 8: ...idi Dish35mm low Corning 35x10mm MatTek P35G X 14 X Willco 35x10 BD Falcon 35x10 Figure 8 Ring selection scheme for 35 mm dish MatTek P50G X 30 X Grainer Petri 60x15 Willco60x15 BD Falcon 60x15 Ibidi...

Page 9: ...etic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder 1 2 Figure 10 Magnetic lock for 1 x 3 chamber slide 1 3 2...

Page 10: ...ushing silicon tubing onto brass opening Gas Input Figure 12 Connection with gas supply 8 Working with Perfusion The H201 UP features 12 perfusion holes on the sides for the insertion of perfusion tub...

Page 11: ...it by the four pan head screws M2 5x6 the screws type depends on the working stage The place of the pan screws is indicated by letter A See Figure 14 Image 1 2 Place the chamber on the stage and fix i...