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NuAire NU-5800, Operation And Maintenance Manual

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OM0211 

Page 1 of 48 

 

 

 

 

 

 

 

 

Air-Jacketed DH Invitrocell CO

2

 Incubator 

 

Models 

NU-5800 (Series 1) 

NU-5800E (Series 1) 

 

Operation and Maintenance Manual 

 

 

 

 

March 2013 

Revision 3 

 

 

 

 

 

 

 

 

 

Manufactured By: 

NuAire, Inc. 

2100 Fernbrook Lane 

Plymouth, MN  55447 

Toll-Free: 1-800-328-3352 

In Minnesota:  (763)-553-1270 

Fax: (763)-553-0459

Summary of Contents for NU-5800

Page 1: ...ll CO2 Incubator Models NU 5800 Series 1 NU 5800E Series 1 Operation and Maintenance Manual March 2013 Revision 3 Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth MN 55447 Toll Free 1 800 328 3352 In Minnesota 763 553 1270 Fax 763 553 0459 ...

Page 2: ...ration 9 2 Door and Perimeter Heater Adjustments Affecting Temperature Calibration 9 3 CO2 Calibration Section No 10 Maintaining Your DH Invitrocell 10 1 Chemical Decontamination 10 2 Shutting Down the Incubator 10 3 In Filter Change Notification Section No 11 Error Indicators Troubleshooting Section No 12 Communication Outputs Section No 13 Electrical Environmental Requirements Insert Replacement...

Page 3: ...volume as a percentage in the chamber for monitoring and control 1 3 Incubator Control Electronics The NuAire Incubator Control Electronics is a state of the art microcomputer based control system specifically designed to service the precise control requirements of the chambers environment providing optimum programmable conditions for culture growth The microcomputer is user friendly and provides ...

Page 4: ...ects fresh air into the chamber at a preset duty cycle to reduce condensation forming on the chamber walls and front glass door The air injections are settable by the user to help control condensation See section 8 8 for this option 1 7 Cabinet Ventilator Fan An axial fan is mounted to the bottom cover panel of the Incubator and runs continuously when the unit is switched on This fan pulls air int...

Page 5: ...t in death of serious injury CAUTION Safety alert symbol indicates a potentially hazardous situation which if not avoided may result in minor or moderate injury WARNING Note Biohazard Hazardous Gases Personal Protection Equipment Required Chemical Hazard Ground Earth Attention accompanies information or important symbol Potential electrical hazard only qualified person to access ...

Page 6: ...8 Rev 3 March 2013 CAUTION Hot Surface Burn Potential Flammable Hazard Lead Free CAUTION used without the safety alert symbol indicates a potentially hazardous situation which if not avoided may result in property damage ...

Page 7: ...d bottom surfaces of the inner chamber Most electronics motors pumps and valves are fully accessible from the back of the unit A CO2 sample port is provided on the front panel just below the door to check the concentration of CO2 in the chamber The CO2 percentage is controlled by a solid state gas infrared sensor using a single light source with dual wave length detection providing accurate monito...

Page 8: ...ance Parameters Temperature Range 5 C to 55 C 5 C above ambient to 30 C max ambient Temperature Uniformity 0 35 C 37 C Temperature Accuracy 0 1 C CO2 Range 0 1 to 20 0 0 set point idles system CO2 Accuracy 0 1 CO2 Recovery Up to 5 0 50 in 5 minutes average Temperature Recovery 0 12 C minute Average Temperature Display Resolution 0 1 C CO2 Display Resolution 0 1 Door and Perimeter Proportional base...

Page 9: ...stainless steel shelves Eight 8 stainless steel shelf brackets One 1 water pan Gas tube Access port plug with breather hole One 1 2 meter 6 5 ft electrical cord Operation and Maintenance Manual Operating Instructions 3 4 Consult Factory for additional Accessories Ordered Separately ...

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Page 11: ...down using an Associated Research Model 7564SA Quadchek II 8106 Omnia 6 or 8006 Omnia 6 or other models Grounding Continuity The resistance between the green bonding conductor of the supply cord and any dead metal part of the cabinet shall not exceed 0 10 ohms 4 2 Functional Tests These functional tests are performed on every Incubator over a 48 hour final acceptance test burn in period All failur...

Page 12: ...ire for a Return Authorization Number This number must accompany all returns Any product shipped to NuAire without this number will be returned refused shipment or collect freight 6 0 Shipments NuAire Inc takes every reasonable precaution to assure that your Incubator arrives without damage Motor carriers are carefully selected and shipping cartons have been specifically designed to insure your pu...

Page 13: ...n addition the Incubator location should address access to its power cord for connection disconnection if necessary For maintenance and service purposes the control center electronics should remain accessible Leave at least 3 ft of clearance in front for the swinging door 7 2 Leveling Prior to use the incubator should be leveled using a bubble level on a middle shelf in the chamber The Incubator s...

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Page 15: ... under the hinged Valance that is beneath the door One 50mm polypropylene 3 micron HEPA filter is connected to the hoses The filter is labeled Air filter To open the valence the outer door must be open Reach in under the front of the valence and pop it open by pulling it toward yourself See BCD 15180 CAUTION This is a free open air atmospheric air supply DO NOT connect to a pressurized air source ...

Page 16: ... regulator First Open the CO2 cylinder slightly for an instant this is termed cracking the valve This will blow out dust or dirt that may have collected in the valve outlet BE SURE to keep your face away from the valve outlet to protect your eyes from dust or dirt Second Make sure the regulator pressure adjusting screw is released by turning it counterclockwise until it turns freely Third Attach t...

Page 17: ...water pan filled the shelves in place and the unit is leveled Read section 8 and follow all instructions for setting set points at the desired value Then calibrate the Incubator control systems as explained in section 9 If the Incubator is installed and calibrated correctly it will meet the performance specs listed in section 3 2 7 10Reversing Incubator Hinges Reversing the incubator hinges from t...

Page 18: ...NDED since it is difficult to change the water weekly and almost always necessitates the use of chemicals which are not recommended and may damage the stainless steel Also it promotes condensation on the inner walls because it steals the natural convection heat flow through the inner chamber and condensation points occur Note ABSOLUTELY NO CHLORINATED OR HALOGEN MATERIALS ARE TO BE USED IN THE CHA...

Page 19: ...nhanced information presentation on a Nu Touch LCD Main Screen Continuously displays Measured Chamber Temperature Measured CO2 level in the chamber Current Screen ID MAIN SCREEN A mode status indicator RUN or STANDBY NuAire Logo button Leads to the System settings Or Password menu when pressed Date and Time Air CO2 inject door ajar status displays An Alarm Status Menu 8 3 3 Active buttons on the M...

Page 20: ... and returns back to the calling screen Cancel This command will cancel the changes you have made and in some circumstances cancels the set of requests that you have entered and returns you back to the calling screen e g Cancel in the middle of some of the CO2 zero span screens returns you to the calling screen Start This command causes a main event to begin e g CO2 zero span calibration Next Step...

Page 21: ...oftware Revision Display Settings Allows adjustment of the screen brightness Set Password A 4 digit password can be set and will protect access to Environmental Set points and changing the password 8 3 6 Automatic notification of abnormal situations See Section 11 for more detail The red Alarm Status text message button will appear on the Main Screen to indicate a fault within the Incubator system...

Page 22: ...e logs and an event log that indicates when alarms door openings and power interruptions occur 4 20 milliamp analog Output RS 485 2 way communications A USB method of updating the software 8 3 9 Diagnostic and calibration assists To access the Service Settings Menu perform the following steps NuAire Logo Service Settings 9876 Enter You are now in the Service Settings Menu screen This screen provid...

Page 23: ...t above the AIR CO2 status messages paragraph 8 4 2 and 8 4 3 This is a visual alert only no alarm will sound Press the Maintenance Required button to go to the screen that will tell you what maintenance should be performed See section 10 for more detailed information regarding this Notice 8 5 Rear Panel Detail The rear control panel contains the following functions BDC 15669 8 5 1 Power Cord The ...

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Page 25: ... be changed following the instructions found in Maintenance Section 10 8 6 2 Air Inlet Filter All models The Incubator is provided with clear vinyl tubing and a 0 3 micron HEPA filter This is a free air supply Note DO NOT CONNECT TO A PRESSURIZED SOURCE 8 6 3 Chamber sample port The Sample port is provided for checking the CO2 level in the chamber to monitor the calibration the Sensor and determin...

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Page 27: ...arm becomes active and the Alarm Status button will appear This could be a catastrophic alarm condition which could harm the tissue culture cells It does indicate an operational abnormality and should be checked To acknowledge the abnormality simply press the Alarm Status button to view the alarm to dismiss it press the corresponding alarm s button The audio and visual alarms will then extinguish ...

Page 28: ...one function will reset the timer but both functions will remain on Output Input Diagnostic Parameter Functions Temperature 1 Safety Relay on off 2 Chamber Temp Sensor shows current temp 3 Safety Temp Sensor shows current temp 4 Chamber heater Triac control 0 50 100 phase firing 5 Door Heater Triac control 0 50 100 phase firing 6 Perimeter Heater Triac control 0 50 100 phase firing CO2 1 CO2 Senso...

Page 29: ...l show ON or OFF corresponding to the valve condition On for open or set or yes 3 CO2 Sensor Bay Heater Triac Condition This function shows on LCD the current state of the CO2 Heater triac LCD will show 0 50 and 100 percent corresponding to the relay position The triac can be toggled to each percent of phase firing manually 4 CO2 Sensor Bay Temperature Sensor This sensor shows the sensor bay tempe...

Page 30: ...m Above Set point This value determines the maximum deviation measured in C that the chamber is permitted to rise above set point once it is reached before an alarm condition is declared An alarm condition will cause the chamber to enter a safe condition where no power is enabled to any of the system output controls until the situation is rectified 2 Temperature Time Out This value determines the ...

Page 31: ...e door heater to a 50 percent duty cycle after an inner glass door opening Also inhibits CO2 control for the same period 2 Air Inject Time This value specifies the time in seconds for an injection of air into the chamber 3 Air Inject Cycle Time This value specifies the amount of time in minutes between each injection of air into the chamber 4 Digital Output Interval This parameter specifies the fr...

Page 32: ...odel number in the System information screen Models NU 5810 NU 5820 NU 5830 NU 5840 and E or D models Enables Temperature and CO2 plus RH and O2 control systems as available on the various models Chooses the algorithm suited for high watt heating of the chamber suited for heated Decon cycles Activates the heated decon cycle choices in the Systems Settings screen Touch the Model Number text then fo...

Page 33: ... not place the glass beaker on the bottom of the chamber because it will result in a slightly higher temperature reading due to the heater located on the chamber bottom Placing the thermometer in glass beaker on the middle shelf will give the most accurate results for calibration The chamber should be humidified to avoid false low readings due to evaporation of water from the flask An accurate dig...

Page 34: ...ts screen either from the MAIN SCREEN or the SYSTEM SETTINGS screen Select the numerical digits following the Door or Perimeter text Press or keys to increase or decrease the numerical value to the desired level Press Done to set current value and exit calibration 9 2 1 Door and Perimeter heater duty cycle automatic control The door and perimeter duty cycles are automatically reduced when the room...

Page 35: ...Settings enter the CO2 Calibrations menu Press the CO2 Offset Calibration text button Use an independent instrument to determine actual CO2 percentage compare the display CO2 to the independent measurement If these two readings have a difference of less than 1 0 percent proceed to enter the CO2 Fyrite value See Note below If the difference is greater than 1 0 percent it is recommended to perform a...

Page 36: ...n 9 4 1 9 4 5 CO2 Injection Calibration The CO2 injection calibration can be performed separately from zero span calibration to optimize the gas injection time required to recover the CO2 level to set point after a door opening The recovery time should be as minimal as possible with virtually no overshoot Verify the CO2 sensor calibration prior to performing an injection calibration The injection ...

Page 37: ... The Air Supply Filter should be replaced every fifth empty CO2 tank or when the filter is visibly discolored yellow brown Air Pump Filters P N X 980366 50mm Disk Uni Directional In Line Wet The Air Pump Filters should be replaced EVERY TWO YEARS to assure optimum performance A visual check should be performed during CO2 sensor calibration to assure filter integrity Remove the sensor housing cover...

Page 38: ...o zero Pressing the Maintenance Required button produces a screen indicating that the timer for filter life has expired Responding to the notification From the main screen press the NuAire Logo Service settings General Option Settings Filter Maintenance to access the Filter Maintenance Adjustments screen In this screen you can extend the alarm by 1 month by pushing the Extend button Reset the noti...

Page 39: ...il Diagnostics is entered or another alarm becomes active Step 2 CLEAR ERROR INDICATORS Error indicators can be cleared by pressing the Alarm Status button and entering the Alarms Menu and pressing the Clear All button if the error is still present the indicator will return when the alarm time reaches 0 Step 3 MONITOR REOCCURRENCE OF ERROR INDICATORS If reoccurrence of the error indicator is immed...

Page 40: ...n Run Cal Inj Calibration see Section 9 3 3 Check replace CO2 gas supply tanks Check for leaks in chamber inner door gasket chamber blower fan shaft seal Check for leaks in air pump and hosing Cal Inject Failed Cal inject calibration failed Not enough increase in the CO2 reading after gas was injected Check gas supply then run calibration again Call NuAire Technical Services if error persists Gene...

Page 41: ...p Remove top plenum and ensure that blower wheel is not rubbing on anything Check jacket fan mounted in bottom on Incubator For further assistance call NuAire Customer Service at 1 800 328 3352 or 763 553 1270 USA 12 0 Communication Outputs 12 1 Remote Alarm Contacts The NuAire DH Incubator contains a set of contact points to connect to a remote alarm system The contacts are located on the rear pa...

Page 42: ... industrial laboratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply 13 5 Pollution Degree 2 0 Pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 0 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensatio...

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