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Fluorescence Imaging
106
http://machinevision.navitar.com
• email: [email protected]
ZFL Video Scope
• Transgenic organisms.
• All GFP (Green Fluorescent Protein) stud-
ies involving living cells and tissues: C.
Elegans, D. Melanogaster, D. Rerio Larva,
Zebra Fish, Oocytes, Nematodes, Droso-
philia.
• Plant cells, plant surfaces, soil samples,
parasites.
• Air and water pollution.
• Forensic - fingerprints, fibers, documents,
and body fluids.
• Capillary flow.
• Bacteria on agricultural products.
• Art restoration.
• Other non-destructive testing.
• Wafer Contamination by organic residue.
• Inspection of photoresist.
• Diagnosis of lumber diseases.
• Inclusions, imperfections, and compound
variation in geological crystals (including
the gem industry).
• Inspection of the structure and chemical
composition of concrete.
• Investigation of the presence and disper-
sions of additives or impurities in polymers
and ceramics.
• Investigation of structural abnormalities in
materials (cracks, pores, welds).
• Confirming presence of adhesives in
cemented applications.
Ideal Uses
Navitar Video Fluorescent Scope
Navitar’s Video ZFL Scope is a Macro/Micro
fluorescent vision system that utilizes inter-
changeable professional fluorescent cubes
and internal focus to create an image compat-
ible with most existing camera systems. It is
a simple means of doing very sophisticated,
task oriented fluorescence without the ex-
pense and complexity associated with a fully
loaded research microscope.
Basic Components of Any Video
Fluorescent System
1. A
light source
emitting the wavelengths
required to cause the labeling dye to
fluoresce.
Two different remote light sources are
available, a halogen light for the longer
wavelengths, and a metal arc lamp for the
UV. LED illumination is currently under
development.
2. An
integrated cube
that optimizes per-
formance by stopping all but the desired
(excitation) wavelength from reaching
the object and then stopping all but the
fluorescing wavelength (emitting) from
reaching the camera. There is a multitude
of off-the-shelf cubes available depending