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3.2.1. Sample Loading for the Prometheus NT.48
The Prometheus NT.48 capillary tray provides 48 numbered slots for single capillaries, offering
maximum flexibility in assay design. It is important that the capillaries are positioned in the
center of the tray. It is also important that the meniscus of the sample does not approach the
heated capillary tray surface, since this will lead to rapid evaporation (
).
Figure 3: Capillary tray layout and loading for the Prometheus NT.48 instrument.
The capillary tray can hold
up to 48 capillaries, which are numbered from 1 to 48, starting in the front closest to the user. When filling the
capillaries, it is important that the capillaries are filled completely, and that they are centered on the capillary tray.
Once all capillaries are loaded onto the capillary tray, place the magnetic lid to fix the capillaries.
3.2.2. Manual Sample Loading for the Prometheus NT.Plex
The Prometheus NT.Plex instrument is designed for fast, automatable high-throughput
measurements. Sample loading can be performed automatically (see 3.2.3) or manually, as
described in this section. The capillaries on Prometheus NT.Plex nanoDSF Grade 24-Capillary
Chips are spaced to enable easy and fast sample loading from 384-well microtiter plates. The
Capillary Chip Filling Station (Cat# NT-AT100) is recommended for manual loading of capillary
chips from microtiter plates since it places the sample at the optimal angle for loading (see