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Guangzhou Micro-shot Technology Co., Ltd

MF43-N

Research grade fluorescence microscope

User Manual

Website

：

www.m-shot.com

Tel:0086-20-38262481

Email: [email protected]

Summary of Contents for MF43-N

Page 1: ...Guangzhou Micro shot Technology Co Ltd MF43 N Research grade fluorescence microscope User Manual Website www m shot com Tel 0086 20 38262481 Email sales mshot com ...

Page 2: ...Research grade fluorescence microscope MF43 N ...

Page 3: ...n improper bulb fuse or power cord may cause damage or fire to this unit Any extended power cord used must be grounded PE 5 Protect this unit from high temperatures dampness and foreign objects To prevent short circuit or any other fault do not expose this unit to any high temperatures or dampness environment for a prolonged period of time A suitable operating environment is designated at a temper...

Page 4: ...ion of bulb Do not touch the glass surface of the bulb directly with bare hands When mounting the bulb wear gloves or wrap it with cotton material Wipe off any dirt on the surface of the bulb with a clean cotton fabric dipped in alcohol If the dirt is not thoroughly removed it would etch the surface of the bulb weakening its brightness and shortening its life Mount the bulb with care to avoid slip...

Page 5: ...th 11 Ⅲ Installations steps 9 1 Install LED lamp house 9 2 Install objective nosepiece 10 3 Install cable 10 4 Install condenser 11 5 Install stage 11 6 Install gripper 11 7 Install fluorescence attachment 12 8 Install fluorescence light box 12 9 Fluorescent module installation cable 12 10 Install eyepiece tube 13 11 Installing eyepiece 13 12 Install adapter 13 13 Install the power cord 14 Ⅳ Broad...

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Page 7: ...1 Field diaphragm Eyepiece Trinocular tube Fluorescence filter wheel Host Fluorescence illuminator lamp house Fluorescence light barrier plate X Y stage hand wheel Ⅰ Components ...

Page 8: ...2 Camera adapter need to buy separately Focusing wheel Light intensity rotate knob Fluorescence illuminator Objective Stage Bright field illuminator power button ...

Page 9: ...turn the low magnification objective into the light path to focus the sample 2 Turn the brightness adjustment knob to select the best observation brightness Press the LIM setting button set key to input the current brightness 3 Repeat the above steps to set the best brightness for each objective Each objective can be set a special brightness separately After setting the brightness for the objectiv...

Page 10: ...to neutral 5 Rotate the coarse and fine focus system to focus the sample 6 Moving the stage control hand wheel to adjust the observation sample area 2 2 Microscope adjustment 1 Adjust the pupil distance adjust the binocular lens tube to the left and right till the right field of view is completely coincident Adjust the diopter turn the diopter adjusting rings on both sides to the scale 0 turn the ...

Page 11: ...wearing glasses put the goggles in the normal folded position to prevent eye contact and scratching When not wearing glasses open the folded eyepiece rubber clap in the direction of the arrow to prevent the external light between the eyepiece and the eye from entering 2 3 Adjust alignment 1 Put on the sample 2 Rotate the objective nosepiece and select 10x objective 3 Turn the coarse and fine focus...

Page 12: ...erture image to the center of the field of view 8 Rotate the adjustment ring of the field of view diaphragm to make the field of view diaphragm connected to the field of view Note do not screw the fixing screw of the condenser in the middle of the alignment knob wrong 2 4 Adjustable aperture diaphragm 1 Rotate the adjusting ring of the numerical aperture of the condenser to make the numerical aper...

Page 13: ...ter locking the coarse focus limit device the coarse adjustment travel will be limited to the position set by the limit and cannot rise to the height beyond the limit 5 Loose and tight device for coarse and accurate focus adjust coarse and accurate focus rotate focus rotate focus 6 The tightness of the knob as it turns The coarse focusing device has been adjusted in advance and is easy to use If n...

Page 14: ...he x axis and y axis knobs Hold the x axis knob screw the y axis knob upward to expose the knob rod 1 Turn the x axis adjusting knob or y axis adjusting knob clockwise arrow direction to increase the tension and turn the y axis adjusting knob counterclockwise to decrease the tension If the tension is adjusted too tightly the clucking sound can be heard when the stage rises or falls or the accuracy...

Page 15: ...tage bracket and stage Please keep the removed screws properly for reuse Use oil mirror 1 Focus the sample in the order from low magnification objective to high magnification objective 2 Before using the oil lens place a drop of oil on the area to be observed of the sample 3 Turn the objective nosepiece into the optical path and adjust the fine focus button Bubbles in the oil will affect the obser...

Page 16: ...to replace the objective nosepiece 3 Hold the objective lens nosepiece keep the dovetail mounting port horizontal insert it into the dovetail on the microscope arm and gently push it into the end 4 Tighten the fixing screw of objective lens turntable When installing or removing the objective nosepiece with cable the cable must be removed in advance 3 3 Installing cables Pass the cable under the mi...

Page 17: ...into the innermost part along the dovetail slot 5 Tighten the fixing screw of the condenser and then raise the condenser to the highest position 3 5 Install the stage 1 Place the stage gently on the stage bracket of the microscope body and clip it into the fixed position of the bracket to prevent the stage from shaking up and down 2 Tighten the fixing screw of the stage 3 6 Install the gripper 1 G...

Page 18: ...hten the right inner hexagon screw 3 8 Installation of fluorescent light box Clip the mercury lamp box into the interface behind the fluorescent module and tighten the two fixing screws on the interface 3 9 Fluorescent module installation cable Plug the cable of the light box into the interface of the fluorescent power control box and connect the ...

Page 19: ...eces into the eyepiece barrel until the end When using the eyepiece with micrometer the eyepiece can be installed in the right eyepiece barrel 3 12 Install the adapter Insert the plug of the power adapter into the socket of the microscope When bending and winding the power cord is easy to be damaged do not use too much force Be sure to turn off the main switch before connecting the power cord Alwa...

Page 20: ...14 3 13 Install the power cord 1 Firmly insert the power cord plug into the input socket of the adapter ...

Page 21: ...t sources corresponding to UV 360 380nm B 430 465nm and G 505 545nm respectively 4 1 Basic operations 1 Turn off the lower light source used in the bright field observation and insert the power plug of the power control box into the power socket Before insertion check whether the power supply voltage is consistent with the power supply voltage required by the instrument ...

Page 22: ... band light source The number above the knob shows the driving current and driving voltage of b band and G band LED lamps 4 2 Installation method 1 Clip the broad spectrum LED light box into the interface at the back of the fluorescent module and tighten the two fixed hexagon screws on the interface After connecting the wide spectrum LED light source and the power control box through the connectin...

Page 23: ...mon No power on Turn on the switch of bright field Mercury lamp No power is connected Turn on the switch of mercury light source The light bulb burned out Mercury lamp The bulb model used is wrong Please use the correct type of bulb Voltage instability Installing the voltage regulator The bulb has reached its life limit Replace the appropriate bulb The field of view is dark Common LIM setting is n...

Page 24: ...ned Adjust the light path alignment of mercury lamp The field aperture and aperture aperture aperture of the fluorescent module are not large enough Adjust to the right size The bulb has reached its service life Replace the bulb The brightness does not change Common LIM function is turned on Turn off LIM function The condenser is not properly aligned Re centering the condenser The condenser is too...

Page 25: ...nser The condenser is not properly aligned Re centering the condenser The field of view aperture is too small Open the field of view aperture until the field of view is circumscribed See dirt or dust in the field of view Common Dirt or dust on eyepiece Wipe it all clean There is dirt or dust on the surface of the condenser There is dirt or dust on the sample There is dirt or dust on the filter in ...

Page 26: ...removal There is dirt or dust on the sample Clean up There is dirt or dust on the condenser Fluoresce nce The condenser drops too high Adjust the height of the condenser to the minimum The aperture is too large Reduce the aperture to a minimum The sample is not ideal Sample preparation or replacement The dye does not match the fluorescence band Consult the manufacturer and recommend the appropriat...

Page 27: ...t installation of objective turntable Push the dovetail joint all the way to the inside The objective nosepiece is not in place Make sure that the objective nosepiece is in the position of the click sound card The condenser is out of alignment Re centering the condenser The focus knob is difficult to turn Common The adjustment of the tightening device of the focusing knob is too tight Loosen the a...

Page 28: ...l the time Common fluorescen ce The coarse collimation knob limit device blocks the stage Turn on the limit device of coarse focusing knob Coarse focus can never be reduced Common fluorescen ce The condenser frame is too low Raise the condenser frame Before focusing the objective collides with the sample Common fluorescen ce The sample is upside down Install the sample correctly The field of view ...

Page 29: ...icroscopic observation When viewing from the eyepiece look at the whole field of view before focusing on the sample range X axis and y axis knob are too tight or too loose Common The tension in the horizontal direction of the x axis or y axis knob is too high or too low Adjusting tension Please operate with both hands carefully when installing and be careful not to drop and damage the light source...

Page 30: ... the fuse is thin glass Please operate carefully when opening the drawer of the fuse box and ejecting the fuse Ⅵ Specification sheet Item Specification Microscope frame Optical system Universal Infinity corrected optical system Structure Original from international famous brand microscope Observation Super widefield trinocular head Wide field eyepiece 10X FN25 diopter is adjustable Revolving nosep...

Page 31: ...l stage with left or right hand low drive control with rotating mechanism and torque adjustment mechanism optional rubber grips available Travel distance 80mm 50mm 1mm per scale accuracy 0 1mm Focus 25 mm stage stroke with coarse adjustment limit stopper torque adjustment for coarse adjustment knobs stage mounting position variable minimum adjustment gradations 1 µm Transmitted illumination system...

Page 32: ...orking distance 17mm 1 UPLFLN10X2 0 3 working distance 10mm 1 UPLFLN20X 0 5 working distance 2 1mm 1 UPLFLN40X 0 75 working distance 0 51mm 1 UPLFLN100XO2 1 3 working distance 0 2mm 1 Phase contrast components M PCD BX 1 PLCN10X 0 25PH working distance 10 6mm 1 PLCN20X 0 4PH working distance 1 2mm 1 PLCN40X 0 65PH working distance 0 6mm 1 Polarizing components Optional 1 Dark field components Opti...

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