Portable
Fluorometer
Operation
Manual
Chapter 8
Accessories
25
No.
Item
Unit
Qty
Remark
1
Power adapter
EA
9V 1.5A
2
0.5ml PCR tube holder
3
User manual
4
Usb stick
Page 1: ...Operation Manual UT FLU1 SERIES Portable Fluorometer PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC 4 22 ...
Page 2: ...peration guide please read carefully before operating it Keep user manual for later use in case you meet with difficulties Opening Check Please check the instruments as well as all accessories with packing list when you first open it If you find anything wrong or missing please contact distributor or manufacturer ...
Page 3: ... Indoor use only Read this manual carefully before operating the instrument Only the qualified person who have the skill or experience can operate it Disassemble the instrument without permission is forbidden except the parts that could be disassembled in the manual Or you will lose the warranty qualification and may have the risk of electric shock Do not cover anything on power wire in operating ...
Page 4: ...ng cases and contact supplier or skilled maintenance to manage There is some liquid flowing into the Instrument Drenched or fire burned Instrument dropping or outer shell damaged Malfunction The Instrument should be put in dry place less dust far away from water and high intensity light What s more the place should be well ventilated no corrosive gas or strong magnetic interference far away from h...
Page 5: ...llation Conditions 4 4 3 Installation Steps 4 4 4 Operating Considerations 4 Chapter 5 Software Introduction 6 5 1 Self Testing 6 5 2 Home Interface 6 5 2 1 Fluorescence Detection 7 5 2 2 dsDNA Detection 9 5 3 Report 17 5 4 Settings 19 5 4 1 Time 19 5 4 2 LCD 20 5 4 3 Print 21 5 4 4 Updata 22 Chapter 6 Maintenance Storage and Transportation 23 Chapter 7 Fault Analysis and Treatment 24 Chapter 8 Ac...
Page 6: ...f low concentration such as protein enzyme receptosome antibody nucleic acid hormone steroid thyroid hormones medicine and microorganism UT FLU1 fluorometer is based on fluoroimmunoassay to detect the luminous intensity of fluorescent reagent in immunoassay IA Under the condition of low concentration the sample concentration is linear to fluorescence intensity Thus the testing sample can be analyz...
Page 7: ...ission wavelength UT FLU1A Flurometer UV LED 365 20nm 420 480nm 60nm Blue LED 460 20nm 525 570nm 45nm UT FLU1B Flurometer Blue LED 460 20nm 525 570nm 45nm Red LED 625 20nm 670 725nm 55nm UT FLU1C Flurometer Blue LED 460 20nm 525 570nm 45nm Green LED 525 20nm 575 640nm 65nm Model Parameters UT FLU1A UT FLU1B UT FLU1C Light source Monochromatic LED Power 5 W Linearity 2 R 0 995 Repeatability 1 5 Sta...
Page 8: ...re This chapter introduces the structure of instrument If it is used for the first time please read this chapter carefully to make a better preparation Structure 1 Fig3 1 Structure1 Structure 2 Fig3 2 Structure2 Lid Printer port USB port Power socket PCR tube base Sample chamber Display Screen Switch ...
Page 9: ... instrument appearance if there is cracks damage or deformation 4 2 Installation Conditions Put on the dry clean and horizontal worktable Working conditions Clean air without corrosion steam or dust Temperature between 10 C to 40 C 4 3 Installation Steps 1 Take out the instrument on the worktable slowly and gently 2 Switch off the instruments with the button on the back of instrument Take out the ...
Page 10: ...g Fig4 1 Sample Test Steps To insure the effect of test results volume of sample should be 3ml for cuvette o 1ml for 0 2ml fluorescence PCR tube and 0 2ml for 0 5ml fluorescence PCR tube Please use corresponding solvent diluting sample to reach the require volume if sample is not enough Pay attention to that concentration range should be within the limit of detection and the sample volume should b...
Page 11: ...ll start self testing once powered on Fig5 1 Starting Interface 5 2 Home Interface Instrument enters into below home interface after self testing finished Fig5 2 Home Interface There are three function icons on the top of the interface Touch each icon into corresponding interfaces Home Interface as default interface after self testing ...
Page 12: ...ve building or concentration analysis But you can set and calibrate standard curve detect sample by clicking the other five icons The function of the five icons is same It is convenient for you to manage datas of different kinds of sample 5 2 1 Fluorescence Detection Click icon Fluorescence to enter fluorescence detection interface as Fig5 3 Fig5 3 Home Fluorescenter In this interface fluorescence...
Page 13: ...er normal detection mode of fluorescence detection Fig5 4 Fig5 4 Home Fluorescenter Blue 460nm 460nm Kinetic Detection Mode Click the button to enter 460nm kinetic detection interface as below Fig5 5 Fig5 5 Home Fluorescenter Blue 460nm Kinetic You can set interval time and total run time via click and of kinetic detection Then click Detecting to run kinetic detection Fig5 6 ...
Page 14: ...the kinetic curve Fig5 7 Fig5 7 Kinetic Detection Curve NOTE Different channel has different excitation and emission wavelength see the details in Chapter 2 5 2 2 dsDNA Detection NOTE The software functions of dsDNA RNA Protein Oligo and Custom are same so this manual only introduce the software function of dsDNA detection Click dsDNA to enter the interface as below Fig5 8 ...
Page 15: ...le NOTE If there is no standard curve this item is not available Standard Curve Use standard sample to build standard curve for calculating sample concentration Calibration Curve Calibrate standard curve to eliminate deviation of instrument in drifting Name of Curve There are five default names of curves dsDNA 01 dsDNA 05 Click pull down menu to build new curve or choose the existing curve Fig5 9 ...
Page 16: ...as exported from UT FLU1 to the U flash disk before Click Im curve to choose the curve data you want to import Fig5 10 Export Curve Export curve which is useless for the moment to U flash disk Click Ex curve to export curve data you need Fig5 11 Delete Curve Click Del curve to delete curve data Fig5 12 Back to the main interface Fig5 10 Import Curve ...
Page 17: ...5 Software Introduction 12 Fig5 11 Export Curve Fig5 12 Delete Curve 5 2 2 1 Standard Curve Choose a Curve ID or create a new curve to enter Standard Curve interface Fig5 13 In this interface there are three icons Parameter Curve Fitting View Curve ...
Page 18: ...ck Parameter and set excitation or emission wavelength and concentration unit of curves Fig5 14 Fig5 14 Home dsDNA Standard Curve Parameter 2 Curve Fitting Click Curve Fitting and standard samples are detected to fit standard curve You can set concentration of standard sample in this interface then check the fitting curve ...
Page 19: ... fitting standard curve and one average value at least Check standard curve Check standard curve when fluorescence values of standard sample are over three Fig 5 16 Fig5 16 Curve Checking Click to check current curve sample number concentration and corresponding fluorescence values Click to check list of concentration and fluorescence values of standard sample Click to choose the mode of curve fit...
Page 20: ...e Check the next sample Delete the current standard sample data 3 View Curve View concentration fluorescence values and equation of current curve Fig5 17 Fig5 17 Home dsDNA Standard Curve View Curve Click to view excitation or emission wavelength and setting time of the curve Click to view the data list of standard curve Fig5 19 Fig5 18 View Curve Data ...
Page 21: ...hus the concentration of the original sample will be calculated after detecting Click or to set the volume NOTE Sample volume is 200uL for 0 5ml qPCR tube 100uL for 0 2ml qPCR tube Range of sample volume is 1 20uL Click Detecting to detect after putting test tube into instrument Fluorescence value will be displayed after detecting and concentration will be calculated according to standard curve Sa...
Page 22: ...f samples 1 3 to improve the accuracy of detection Fig 5 21 It also helps to save time and samples of setting standard curve Fig5 21 Calibration Interface NOTE You can prepare 1 3 samples to calibrate the instrument according to the below principles 1 Single point calibration using blank solution to calibrate 2 Two point calibration using a high and a low concentration sample to calibrate 3 Three ...
Page 23: ...fferent kinds of test data Fig5 23 Fig5 23 Report List View the selected results Delete and print the selective data In the view interface Concentration is the concentration of original sample Volume is the volume of original sample added to the test tube Fig5 24 Export all the detecting data to the U disk Print the detecting data you choose Delete the detecting data you choose Delete all the dete...
Page 24: ...ntroduction 19 NOTE Once the data is deleted it can not be recovered Fig5 24 View Interface 5 4 Settings You can set Time LCD Print and Update in settings interface Fig 5 25 Fig5 25 Settings Interface 5 4 1 Time Click or button to set the date and time Fig5 26 ...
Page 25: ... Time Save the date and time you have setted and back to the Settings interface Give up setting and back to the Settings interface 5 4 2 LCD Click LCD to set the screen Fig5 27 Fig5 27 Settings LCD Calibration According prompt message to calibrate accuracy touch spot of the screen Fig 5 28 ...
Page 26: ...ss Fig5 29 Fig5 29 Settings LCD Brightness 5 4 3 Print This instrument is equipped with a thermal printer via USB to connect with Click Print to enter the setting interface Fig5 30 Select the mode of auto print it will print the detecting data after you detect sample every time Save the setting and back to the Settings interface Back to the Settings interface ...
Page 27: ...Software Introduction 22 Fig5 30 Settings Print 5 4 4 Updata Insert the U disk which has stored new version of software and click OK button to update the instrument Fig5 31 You need to restart the instrument to complete update Fig5 31 Settings Update ...
Page 28: ...s Instrument already calibrated before delivery User is not allowed to disassemble the instrument Any deficiency occurs please contact manufacturer Continuous emergency turning on off is not allowed Make sure apply the device with correct input voltage scope Storage and transportation Storage in the room temperature of 10 40 relative humidity less than 80 without corrosive gas and with good ventil...
Page 29: ...ive Check the power supply Check whether the plug is loose 2 Light source can not light up Power of light source failure Module poor contact Check the power Check whether the module is loose 3 Measurement not stable PCR tube isn t in place Check the PCR tube and press it in place 4 Measurement not accuracy Linear of standard curve is bad Instrument drifting Reset standard curve Calibrate standard ...
Page 30: ...Portable Fluorometer Operation Manual Chapter 8 Accessories 25 Chapter 8 Accessories No Item Unit Qty Remark 1 Power adapter EA 1 9V 1 5A 2 0 5ml PCR tube holder EA 1 3 User manual EA 1 4 Usb stick EA 1 ...
