Operation
Manual
Chapter 1
Introduction
1
Chapter
1
Introduction
PCR-IF-8C
Isothermal
Fluorescence
PCR
is
contain
temperature
control
block,
fordoptical
path
detection
system,
heating
lid
and
analysis
software.
It
is
use
d
cannisothermal
amplification
and
fluorescence
real-time
detection
of
samples,
a
s
widelyimake
quantitative
and
qualitative
analysis
of
the
amplification
result.
It
i,gused
in
rapid
detection
of
pathogenic
microorganisms(viruses,
bacteria,
fun
mycoplasma,
parasites
etc.),
food
hygiene
inspection,
environmental
monitoring,
sex
identification
of
animal
embryos
and
detection
of
genetically
modified
food,
etc.
Working
principle:
Temperature
control
block
of
the
instrument
heats
the
sample
seat
to
heat
the
tube
in
the
seat
to
the
needed
temperature.
The
heating
lid
function
can
effectively
inhibit
the
evaporation
of
reagent
and
keep
the
reaction
system
constant
without
adding
paraffin
oil.
The
optical
path
detection
system
can
collect
the
fluorescence
intensity
of
the
DNA
containing
dye
or
probe
in
the
test
tube
at
a
set
time
interval.
The
analysis
software
can
process
and
transform
the
collected
signals,
and
finally
output
the
experimental
results.
Principle
of
optical
path
detection
system:
1: Principle diagram of optical path detection system;
21: Plus lens;
22: Excitation filter; 23: Excitation light source
31: Collecting lens; 32: Emission filter; 33: Detector
Summary of Contents for PCR-IF-8C
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