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8.USING OIL IMMERSION OBJECTIVES
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Oil immersion objectives are labelled with the additional engraving “Oil” and are to be immersed in
oil between the specimen and the front of the objective.
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The immersion oil supplied by Motic is synthetic, non-fluorescing and non-resining oil, with a
refractive index of 1.515.
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The small bottle of oil supplied with every immersion objective facilitates application of the oil to
the cover slip.
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Remove any air bubbles in the nozzle of the oil container before use.
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Immersion oil must be used sparingly. After the examination, the oil should be wiped off the
objective with a lens cleaning tissue and the residual film removed with soft cloth moistened with
petroleum benzene or absolute alcohol.
●
Locate the field of interest with a lower magnification objective. Swing the objective out of the light
path, and add one drop of immersion oil over the site of the specimen.
Swing in the oil immersion objective. There should be a small column of oil from the cover slip to
the objective lens. Use the fine focus to make the image sharp.
●
At operating with Immersion Oil, the space between Objective and Cover class must be free of
any
Air
bubbles
