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35

8.USING OIL IMMERSION OBJECTIVES 

 

 

Oil immersion objectives are labelled with the additional engraving “Oil” and are to be immersed in  

 

oil between the specimen and the front of the objective. 

 

The immersion oil supplied by Motic is synthetic, non-fluorescing and non-resining oil, with a  

 

refractive index of 1.515. 

 

The small bottle of oil supplied with every immersion objective facilitates application of the oil  to  

 

the cover slip. 

 

Remove any air bubbles in the nozzle of the oil container before use. 

 

Immersion oil must be used sparingly. After the examination, the oil should be wiped off the  

 

objective with a lens cleaning tissue and the residual film removed with soft cloth moistened with  

 

petroleum benzene or absolute alcohol. 

 

Locate the field of interest with a lower magnification objective. Swing the objective out of the  light  

 

path, and add one drop of immersion oil over the site of the specimen.  

 

Swing in the oil immersion objective. There should be a small column of oil from the cover slip to  

 

the objective lens. Use the fine focus to make the image sharp. 

 

At operating with Immersion Oil, the space between Objective and Cover class must be free of 

 any 

Air 

bubbles 

 

Summary of Contents for Panthera C

Page 1: ...is needed to operate Motic Panthera Series Microscopes at maximum performance and to ensure safety at all specified operations Please familiarize yourself with the use of this microscope and pay spec...

Page 2: ...familiarize yourselves with the Instruction Manual provided in English language Other Language versions are available as download on Motic web services under the Address http www motic com Panthera Pa...

Page 3: ...ope 9 1 7 Instrument warranty 9 2 Nomenclature 10 2 1 Panthera S 10 2 2 Panthera U C L HD 11 3 Setting up the instrument 14 3 1 Operating environment 14 4 Assembling the microscope 17 4 1 Verifying in...

Page 4: ...p adjustment 27 6 5 Interpupillary distance adjustment 28 6 6 Diopter adjustment 29 6 7 Adjustment of Brightfield Panthera C L 30 6 7 1 Full Kohler Illumination 30 6 8 Use of aperture diaphragm 31 6 9...

Page 5: ...onents 36 9 3 Disinfecting the Microscope 36 9 4 When not in use 36 9 5 Bulb replacement 37 9 5 1 Replacing the 6V 30W halogen module or the two different LED module 37 For Panthera C L HD 9 5 2 Elect...

Page 6: ...e instrument CAUTION Risk of Dangerous heat This symbol indicates a possible hazard to the user of the instrument CAUTION Risk of Electrical Danger Disconnect the plug in power unit from line power be...

Page 7: ...s in compliance with EN 61326 and EMC and Radio noise suppression is in compliance with EN 5501 1 The instruments listed in this document are disposed of in compliance with WEEE Directive 2002 96 EC L...

Page 8: ...surfaces 1 4 Instrument Disposal Please observe the following safety notes for the disposal of the microscope Defective Instruments accessories and consumables should be disposed in compliance with th...

Page 9: ...e only use rated Power cords as described in the Appendix of this Document Always disconnect the power cable before opening the instrument and changing the lamp or LED source Wait for the lamp to cool...

Page 10: ...th the instruction manual those may cause interference to radio communications Operation of this Instrument in a residential area is not permitted FCC WARNING Changes or modifications would void the I...

Page 11: ...10 2 NOMENCLATURE 2 1 Panthera S Binocular Version...

Page 12: ...11 2 2 Panthera U C L Binocular Version HD...

Page 13: ...er Illumination Objective Lenses Magnification N A W D mm Plan UC 2X 0 05 7 2 Plan UC 4X 0 1 30 5 Plan UC 10X 0 25 17 4 Plan UC 20X 0 45 0 8 Plan UC 40X 0 65 0 6 Plan UC 60X 0 8 0 35 Plan UC 100X 1 25...

Page 14: ...which two points can be differentiated Focal depth The area along the optical axis where a focused image appears to be sharp If the aperture iris diaphragm is stopped down the focal depth becomes bigg...

Page 15: ...up to 31 C decreasing linearly to 50 relative humidity at 40 C Supply voltage fluctuations Not to exceed 10 of the normal voltage Pollution degree 2 in according with IEC60664 Installation Overvoltage...

Page 16: ...Light Tracer digital light control Bino trino Plan UC optic Rackless Stage Panthera Classic Bino trino Plan UC optic Rackless Stage Panthera LifeScience Light Tracer digital light control ImagingOnDev...

Page 17: ...Link ImageRecall Similar to Panthera L without Eyepiece Please familiarize yourself with the instructions given in this Operation Manual In case of unresolved questions please contact Motic after sale...

Page 18: ...r to prevent electric shock always turn the power switch on the power supply off before connecting the power cord 4 2 Illumination 4 2 1 Halogen bulb The quartz halogen bulb provides a nearly complete...

Page 19: ...on stages are available for option Left hand operation stages is available for option It should be used with the short stage knob for avoiding to interfere the fine focus knob Fig 2 Stage drive Torque...

Page 20: ...r magnification objective into position Fig 3 4 6 Condenser Raise the stage by turning the coarse focus knob Completely lower the condenser carrier by turning the condenser focus knob Fig 4 1 Insert t...

Page 21: ...ube in place Fig 5 2 Fig 5 1 Fig 5 2 4 8 Eyepieces Use the same magnification eyepieces for both the eyes To secure the eyepiece in the eyepiece sleeve tighten the clamp screws Twist the eyepiece anti...

Page 22: ...in photomicrography ND4 T 25 ND16 T 6 25 Blue filter colour balance filter For routine microscopy and photomicrography Green interference 546nm For phase contrast and contrast adjustment with black an...

Page 23: ...ect APP store via Motic servicing Servers In case of any issue please visit the Website http www motic com Panthera app html Connect the same network with Panthera If Panthera is hotspot SSID format i...

Page 24: ...nnect to Screen Projector Plug your Mouse or Keyboard Take care your data with a USB Stick Connect to remote Location Online consultation and Discussion Digital Classroom Optional WIFI via USB Stick P...

Page 25: ...pe to a touch enabled HDMI Screen and conveniently use a keyboard to enter your reports Plug and Play Accessories 3 Button Wheel mouse for ease of use and roll to zoom USB Keyboard simply connect the...

Page 26: ...w to use QR Link 5 3 2 ImageRecall Panthera ImagingOnDevice stores relevant Data into the EXIF Header of each Image being made User can recall the same Illumination settings using the ImageRecall feat...

Page 27: ...ation on the fine focus knob is 2 microns Never attempt either of the following actions since doing so will damage the focusing mechanism Rotate the left and right knob while holding the other Turning...

Page 28: ...t but will only lower the stage Lower the stage by using the coarse focus knob Fig 10 6 4 Stage upper limit stop adjustment Fig 11 Upper Stage Limit is preset at the factory please only adjust if nece...

Page 29: ...nterpupillary distance bring a specimen into focus using the 10x objective Adjust the interpupillary distance so that both the right and left field of view become one This adjustment will enable the u...

Page 30: ...to the other eye and follow below steps Correct the focus for the second eye by using only the diopter adjustment ring Fig 13 do not use the coarse fine focusing knob Change to a higher magnification...

Page 31: ...to focus the 10x lens on the specimen Look through the second eyepiece 6 and if necessary adjust the diopter until the second eyepiece has sharp image Fully open the Aperture diaphragm using the knur...

Page 32: ...lightly larger than the field of view If the illuminated area is set much larger than the field of view extraneous light will enter the field of view This will create a flare in the image and lower th...

Page 33: ...ination has not been changed for 3s the actual value will be stored for this Objective magnification The light value stored is independently for each objective lens and will be automatically restored...

Page 34: ...turn on Once touch the intensity knob or nosepiece standby model turn off Intensity will be adjusted to the last memorized status LED Indicator shows breathing status to indicate its in standby 6 14...

Page 35: ...definition and contrast To ensure optimal illumination check the position and centring of the lamp and position of the condenser Select a blue filter for routine application An additional colour comp...

Page 36: ...er before use Immersion oil must be used sparingly After the examination the oil should be wiped off the objective with a lens cleaning tissue and the residual film removed with soft cloth moistened w...

Page 37: ...le liquid be careful handling around open flame and always observe the local security advise how to handle such substances 9 2 2Cleaning of painted or plastic components Do not use organic solvents th...

Page 38: ...two different LED module For Panthera C L HD Left up the cover of illumination module Fig 15 1 Loosen the fastening screw of the illumination module In doing so slightly press the screw against the s...

Page 39: ...ED module high color temperature 5500K 6000K LED module low color temperature 3000K 3500K C Panthera C Camera USB Output 5V 0 5A Input AC100 240V 50 60Hz 1 2A Bulb 3W LED or 6V 30W Halogen LED module...

Page 40: ...Field diaphragm closed too far Aperture diaphragm closed too far Improper condenser objective combination Revolving nosepiece not clicked into position Trinocular eyepiece tube optical path selector...

Page 41: ...focality of objectives Eyepiece diopter not adjusted No cohesion of binocular image Magnification or field of view of left and right eyepieces differ Interpupillary distance not adjusted Eyepiece diop...

Page 42: ...e 1 Certified Cord Specifications Voltage Rating 125V AC for 100 120V AC area or 250V AC for 220 240V AC area Current Rating 6A minimum Temperature Rating 60 C minimum Length 3 05 m maximum Fitting Co...

Page 43: ...EMMEQU HAR 10 30 50 British approvals Service for Electric Cables BASEC BASEC HAR 10 10 30 N V KEMA KEMA KEUR HAR 10 30 30 SEMKO AB Svenska Elektriska Matenelkontrollanstalter SEMKO HAR 10 10 50 sterr...

Page 44: ...he thickness of this small glass piece is now standardized at 0 17 mm for most applications Diaphragm Condenser A diaphragm which controls the effective size of the condenser aperture A synonym for th...

Page 45: ...ve Such a liquid is usually required by objectives of 3 mm focal length or less Resolving Power A measure of an optical system s ability to produce an image which separates two points or parallel line...

Page 46: ...arcelona Spain Tel 34 93 756 6286 Fax 34 93 756 6287 Website http www motic com E mail info motic com hk Motic China Group Ltd China Motic Building Torch Hi Tech Industrial Development Zone Xiamen P R...

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