14
4
Microscopy
Manipulation of Each Component
4.1
Coarse and Fine Focusing
Focusing is carried out with the coarse and fine focus knobs at the left
and right of the microscope stand.
The direction of vertical movement of the stage corresponds to the
turning direction of the focus knobs.
One rotation of the fine focus knob moves the stage 0.2mm. The
graduation on the fine focus knob is 2 microns.
Never attempt either of the following actions, since doing so will
damage the focusing mechanism:
Rotate the left and right knob while holding the other.
Turning the coarse and fine focus knobs further than their limit.
I. Coarse Focus Torque Adjustment
To increase the torque, turn the torque adjustment ring located
behind the left-hand coarse focus knob in the direction indicated by
the arrow. To reduce the torque, turn the ring in the direction
opposite to that indicated by the arrow.
II. Coarse Focus Height Stopper
The coarse focus height stopper marks the stage position at which
the specimen is in focus i.e. by restricting the movement of the
coarse focus knob.
With the specimen in focus, turn the coarse focus stopper knurled
screw clockwise until it reaches the stop.
When the coarse focus stopper is in position, the stage cannot be
raised from that position. However, the fine focus knob can move
the stage regardless of the limit but will only lower the stage.
Lower the stage by using the coarse focus knob.
