Molecular Devices SpectraMax GEMINI EM, Operator'S Manual

Page: 63 / 64

Common Wavelengths for Fluorescence and Lumines-
SpectraMax GEMINI EM Operator’s Manual B-3
Values in this table are based on the literature. You may wish to scan
your fl uorochrome of interest in the SpectraMax GEMINI EM to
determine the optimal excitation and emission wavelengths for your
application.
Fluorescence
Luminescence
a
Emerald, Emerald II, Sapphire, Sapphire II, and Ruby are
trademarks of Tropix, Inc.
NOTE:
Fluorophore
Excitation
Wavelength
Emission
Wavelength
HPPA 320 405
4-MeU, NADH, NADPH 355 460
Biotinidinase 355 544
PKU 390 485
Green Fluorescent Protein 390 510
Attophos/Atto fl uor 444 555
FITC 485 538
Ethidium Homodmer (DNA) 530 620
TRITC, Ethidium Bromide 544 590
Texas Red 584 612
TAMRA 547 580
Tryptophan 280 340
La Jolla Blue 695 705
Probe Wavelength
Emerald and Emerald II
a
542
Sapphire and Sapphire II
a
461
Ruby
a
620